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• Original Article •
In vivo detection of the reversal effects using rAd/p53 to human colon cancer in mice with diffusion-weighted magnetic resonance imaging
XIE Qi  WU Min-yi  YANG Yi-ming  ZHANG Ding-xuan  LEI Zheng-xian  ZHANG Jing  XU Jin  ZHONG Wei-de 

DOI:10.12015/issn.1674-8034.2016.01.011.


[Abstract] Objective: To investigate the potential of diffusion-weighted magnetic resonance imaging (DWI) to detect the reversal effects of rAd/p53 for 5-FU resistance of human colon cancer in vivo.Materials and Methods: One hundred nude mice with human colon cancer SW480/5-Fu (5-Fu resistance) were randomly assigned into four groups: control group, 5-FU group, rAd/p53 group, rAd/p53+5-FU. At 24 h, 48 h, 72 h, 120 h and 168 h after treatment, 5 mice were selected randomly from each group and given DWI examinations. Then these mouse models were sacrificed with an overdose of anesthetic. Their tumors were removed and detected apoptosis (TUNNEL).Results: The ADC values at the edge area of the tumor in rAd/p53 and rAd/p53+5-FU groups were significantly higher than that of the control groups at the observed time points (P<0.05). The area ratios of tumor cell apoptosis in the rAd/p53+5-FU and rAd/p53 group were larger than that of control groups at the observed time point. The area ratios of tumor cell apoptosis and the ADC values in the rAd/p53+5-FU group were larger than that of rAd/p53 group at the observed time point. The ADC values of tumors were positively correlated with the area ratios of tumor cell apoptosis.Conclusions: The ADC value can evaluate the reversal effects of drug resistance with rAd/p53 in early stage of therapy in vivo.
[Keywords] Genes, p53；Colonic neoplasms；Fluorouracil；Diffusion magnetic resonance imaging；Animal experimentation

XIE Qi* Medical Imaging Department, Nan Sha Center Hospital, Guangzhou Municipal First People’s Hospital, Guangzhou Medical University, Guangzhou 511457, China

WU Min-yi Medical Imaging Department, Nan Sha Center Hospital, Guangzhou Municipal First People’s Hospital, Guangzhou Medical University, Guangzhou 511457, China

YANG Yi-ming Medical Imaging Department, Nan Sha Center Hospital, Guangzhou Municipal First People’s Hospital, Guangzhou Medical University, Guangzhou 511457, China

ZHANG Ding-xuan Medical Imaging Department, Nan Sha Center Hospital, Guangzhou Municipal First People’s Hospital, Guangzhou Medical University, Guangzhou 511457, China

LEI Zheng-xian Medical Imaging Department, Nan Sha Center Hospital, Guangzhou Municipal First People’s Hospital, Guangzhou Medical University, Guangzhou 511457, China

ZHANG Jing Department of Pathology, Sun yat-Sen University Cancer center, Guangzhou 510080, China

XU Jin Department of Pathology, Guangzhou Municipal First People's Hospital, Guangzhou Medical University, Guangzhou 510180, China

ZHONG Wei-de Clinical molecular medicine and molecular diagnostics laboratories, Guangzhou Municipal First People's Hospital, Guangzhou Medical University, Guangzhou 510180, China

*Correspondence to: Xie Q, E-mail: xieqi8@yeah.net

Conflicts of interest   None.

ACKNOWLEDGMENTS  Natural Science Foundation of Guangdong Province No. 2015A030313732
Received  2015-10-01
Accepted  2015-11-07
DOI: 10.12015/issn.1674-8034.2016.01.011
DOI:10.12015/issn.1674-8034.2016.01.011.

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