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• Original Article •
Quantitative T2 mapping to monitor the process of lumbar intervertebral disc degeneration in a rabbit model
XIE Guang-you  YANG Hai-tao  LÜ Fu-rong  LIU Chang-jie  YANG Mingfang  WANG Rong-pin 

DOI:10.12015/issn.1674-8034.2016.03.010.


[Abstract] Objective: To improve the recognition of quantitative T2 mapping to dynamically monitor the process of lumbar intervertebral disc degeneration in a rabbit model.Materials and Methods: The nucleus pulposus of L2—3, L3—4 and L4—5 discs of 12 rabbits were aspirated by using a 18G needle and the normal L1—2, L5—6 discs of each rabbit and the discs between L2—5 vertebral body of 6 rabbits acted as the normal intra and inter control groups, respectively. The rabbits were performed with sagittal MR T2WI and T2 mapping sequences scanning at the pre-and-post-operative 4 th, 8 th, 12 th week, respectively. The signal intensity of each disc was evaluated and T2 relaxation time was measured carefully. The discs between L1-6 were dissected and then performed with HE and Masson trichrome staining to evaluate the form of discs, content change of nucleus pulposus cells and matrix between normal and experimental groups.Results: The nucleus pulposus could be punctured successfully with a 18 G needle in the root of transverse process via spinal bypass. The normal discs manifested with uniform high signal in T2WI. The signal intensity decreased sharply at the 8 th week and became completely low signal intensity at the 12 th week post-operation. The significant difference of T2 relaxation time found between normal control groups and experimental groups after 4, 8 and 12 weeks was analysed by one-way ANOVA, and the result was F=38.82 (P<0.05). The T2 relaxation time at the 8 th week post-operation was significantly different (P<0.05). The results of HE and Masson staining showed that the cells and matrix decreased gradually, moreover, the boundary between annulus fibrosus and nucleus pulposus became obscure. The nucleus pulposus displayed the process of fibrosis and was almost replaced by fibrous tissue at 8 th week. The discs was fibrocartilage and local cartilage can be observed at the 12 th week.Conclusions: T2 mapping can be used to dynamically monitor the process of lumbar intervertebral disc degeneration.
[Keywords] Intervertebral disk degeneration；Disease models, animal；Magnetic resonance imaging；Animal experimentation

XIE Guang-you Department of Radiology, Guizhou Provincial People’s Hospital, Guizhou Province, Guiyang 550002, China

YANG Hai-tao* Department of Radiology, the First Affiliated Hospital of Chongqing Medical University, Chongqing 400016, China

LÜ Fu-rong* Department of Radiology, the First Affiliated Hospital of Chongqing Medical University, Chongqing 400016, China

LIU Chang-jie Department of Radiology, Guizhou Provincial People’s Hospital, Guizhou Province, Guiyang 550002, China

YANG Mingfang Department of Radiology, Guizhou Provincial People’s Hospital, Guizhou Province, Guiyang 550002, China

WANG Rong-pin Department of Radiology, Guizhou Provincial People’s Hospital, Guizhou Province, Guiyang 550002, China

*Correspondence to: Yang HT, E-mail: frankyang119@126.com. LÜ FR, E-mail: lfr918@sina.com

Conflicts of interest   None.

ACKNOWLEDGMENTS  The paper was supported by the Natural Science Funding of Chongqing No. cstc2011jjA10082 National Clinical Key Subject Construction Project No. 2013-544
Received  2015-12-23
Accepted  2016-01-30
DOI: 10.12015/issn.1674-8034.2016.03.010
DOI:10.12015/issn.1674-8034.2016.03.010.

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