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• Original Article •
MRI tracking study of SPIO labeled bone marrow stromal cells transplantation for treatment of lacunar stroke
HUANG Xiao-lei  ZHOU Guo-xing  WANG Bo-cheng  WU Jin-liang  XUE Yang  CAI Ling-ling  XING Jin  ZONG Gen-lin  ZHAN Qing  ZHAO Jiang-min 

DOI:10.12015/issn.1674-8034.2016.04.012.


[Abstract] Objective: To explore the feasibility of MRI technology in tracing bone marrow mesenchymal stem cells labeled with SPIO, and observe migration and therapeutic condition of BMSCs in focal cerebral infarction model.Materials and Methods: Investigate label efficiency and proliferation activity of three different concentrations of SPIO (25 μg/ml, 50 μg/ml, 75 μg/ml) by Prussian blue staining and CCK8 method. A total of 28 male SD rats were randomly divided into normal control group (CON, n=4), sham operation group(SHAM, n=4), focal cerebral infarction group treated with SPIO only (MCAO+SPIO, n= 10), and focal cerebral infarction treated with optmal SPIO-BMSCs (MCAO+SPIO-BMSC, n=10), MRI scan was underwent on 1 day, 9 days, 20 days, 30 days, 43 days after cell transplantation. Distribution of cell migration were observed by Prussian blue staining.Results: When label concentration was 50 μg/ml, Prussian blue staining efficiency was 100%. Compared with control group and 75 μg/ml group, 50 μg/ml group was significantly different (P<0.05). In MCAO+SPIO and MCAO+SPIO-BMSC groups, transplanted cells on T2WI and SWI sequences obviously show signal reducing area. Compared with T2WI sequences, SWI sequences show a longer, wider range of low signal. Compared with MCAO+SPIO group, MCAO+SPIO-BMSC group can observe that bone marrow mesenchymal stem cells migrate into infarction areas. Prussian blue staining showed that positive cells gathered around the infarction area.Conclusion: MRI techniques can effectively trace mesenchymal stem cells labeled with 50 μg /ml SPIO, monitor migration and therapeutic condition of BMSCs in focal cerebral infarction model, and evaluate the imaging improvement of focal cerebral infarction.
[Keywords] Superparamagnetic iron oxides；Bone marrow mesenchymal stem cells；Stroke；Magnetic resonance imaging

HUANG Xiao-lei The Nine People's Hospital Affiliated to Shanghai Jiao Tong University School of Medicine, Shanghai 201999, China

ZHOU Guo-xing Department of Medical Imaging, Oriental Hospital of Shanghai City, Shanghai 200120, China

WANG Bo-cheng The Nine People's Hospital Affiliated to Shanghai Jiao Tong University School of Medicine, Shanghai 201999, China

WU Jin-liang The Nine People's Hospital Affiliated to Shanghai Jiao Tong University School of Medicine, Shanghai 201999, China

XUE Yang The Nine People's Hospital Affiliated to Shanghai Jiao Tong University School of Medicine, Shanghai 201999, China

CAI Ling-ling The Nine People's Hospital Affiliated to Shanghai Jiao Tong University School of Medicine, Shanghai 201999, China

XING Jin The Nine People's Hospital Affiliated to Shanghai Jiao Tong University School of Medicine, Shanghai 201999, China

ZONG Gen-lin Department of Medical Imaging, Oriental Hospital of Shanghai City, Shanghai 200120, China

ZHAN Qing* Shanghai Seventh People’s Hospital, Shanghai 200137, China

ZHAO Jiang-min* The Nine People's Hospital Affiliated to Shanghai Jiao Tong University School of Medicine, Shanghai 201999, China

*Correspondence to: Zhan Q, E-mail: zhanqing@tongji.edu.cn; Zhao JM, E-mail: johnmzhao@sjtu.edu.cn

Conflicts of interest   None.

ACKNOWLEDGMENTS  This work was part of key projects of Shanghai Municipal Science and technology innovation action plan No. 10411953400 Project of Medicine School of Shanghai Jiaotong University No. 12XJ30061 Project of Shanghai health and Family Planning Commission No. 20124194 Scientific research project of Science and Technology Committee of Baoshan District Shanghai No. 14-E-4
Received  2016-01-04
Accepted  2016-03-11
DOI: 10.12015/issn.1674-8034.2016.04.012
DOI:10.12015/issn.1674-8034.2016.04.012.

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