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• Original Article •
Study of aquaporin magnetic resonance molecular imaging in transient cerebral ischemia rat model
XING Pei-Qiu  CHEN Qiu-yan  WU Fu-lin  PENG Xiao-lan  JIANG Min  CHEN Ting-ting  WEI Ding-tai 

DOI:10.12015/issn.1674-8034.2017.01.012.


[Abstract] Objective: Sought to discern the changes in aquaporin magnetic resonance molecular imaging (AQP MRI) parameter after experimental stroke.Materials and Methods: Thirty-one adult SD male rats were randomly divided into two groups: ischemia group which underwent 1 h right middle cerebral artery occlusion (MCAo) and sham group. T2 FLAIR , conventional DWI and AQP MRI examination was executed 48 h after reperfusion. And AQP4 expression was detected by Western blot and immunofluorescence.Results: The percent change of T2 values of lesions in ischemia group was increased up to 56.655±7.359, which was much higher than that of sham group (vs 2.334±2.203, P <0.01), whereas the homologous ADC values were remarkably decreased (-24.491±1.924 vs-0.960±3.824, P<0.01). Result of AQP MRI showed that the percent change of AQP ADC of ischemia group was significantly decreased than that of sham group(-25.218±8.839 vs 0.209±1.279, P<0.01). Western blot result showed that the AQP4 expression in affected side of ischemia group was higher than that of sham group. Contrastive analysis showed that areas with high AQP ADC values presented low positive staining of AQP4 in immunofluorescence, and areas with low AQP ADC values presented high positive staining.Conclusions: AQP MRI could display the distribution of aquaporin in vivo, which would be of great importance to assess progression of cerebral ischemia.
[Keywords] Aquaporin magnetic resonance molecular imaging；Brain ischemia；Aquaporin；Brain infarction

XING Pei-Qiu Department of Radiology, Fujian Medical University Ningde Hospital, Ningde 352100, China

CHEN Qiu-yan Department of Radiology, Fujian Medical University Ningde Hospital, Ningde 352100, China

WU Fu-lin Department of Radiology, Fujian Medical University Ningde Hospital, Ningde 352100, China

PENG Xiao-lan Department of Radiology, Fujian Medical University Ningde Hospital, Ningde 352100, China

JIANG Min Department of Radiology, Fujian Medical University Ningde Hospital, Ningde 352100, China

CHEN Ting-ting Department of Radiology, Fujian Medical University Ningde Hospital, Ningde 352100, China

WEI Ding-tai* Department of Radiology, Fujian Medical University Ningde Hospital, Ningde 352100, China

*Correspondence to: Wei DT, E-mail: wdtai83@163.com

Conflicts of interest   None.

ACKNOWLEDGMENTS  This study was supported by National Natural Science Foundation of China NO. 81571838 Natural Science Foundation of Fujian Province NO. 2014J01398 Medical Elite Cultivation Program of Fujian, P.R.C NO. 2014-ZQN-JC40 Fujian medical university non-subordinate affiliated hospital scientific research development special foundation NO. FZS13021Y Fujian province health department youth scientific research subject NO. 2013-2-158
Received  2016-06-24
Accepted  2016-11-26
DOI: 10.12015/issn.1674-8034.2017.01.012
DOI:10.12015/issn.1674-8034.2017.01.012.

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