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• Clinical Article •
Evaluation of brain injury in neonates by magnetization transfer imaging combined amide proton transfer imaging: a preliminary study
ZHENG Yang  WANG Xiao-ming 

DOI:10.12015/issn.1674-8034.2017.03.006.


[Abstract] Objective: To evaluate neonatal brain injury at the internal environmental level with the application of amide proton transfer (APT) imaging and magnetization transfer (MT) imaging by measuring the APT and MTR values of the brain.Materials and Methods: A total of 38 neonatal patients who underwent MRI examination were enrolled in the study. Among them, there were 25 newborns with no abnormalities and 13 cases with brain injury who underwent conventional MRI (T1WI, T2WI, DWI) examination. After obtaining informed consent and permission of clinicians, routine MRI was followed by additional APT-MT scan. APT-MT imaging is single slice scanning, performed at the basal ganglia level in all neonates, and in the case group, with increased localization at the level of lesion, and with the contralateral relatively normal area as self-control. The APT/MTR values of bilateral frontal subcortical white matter, basal ganglia and occipital subcortical white matter were measured for all neonates, as well as the APT/MTR values of the lesion and contralateral areas. Several statistical methods were used for statistical analysis.Results: In the control group, bilateral frontal subcortical white matter, basal ganglia and occipital subcortical white matter had no significant difference in APT/MTR values (P>0.05). Between the different parts of the brain, the differences among the APT/MTR of the frontal lobes, basal ganglia, and occipital lobes were significant, P<0.05. In addition, the APT/MTR values of the above brain regions were found to have a positive correlation with gestational age. In the case group, there were significant differences in APT values between the lesion side and contralateral area, being significantly lower in lesion side than the contralateral side (P<0.05).Conclusions: From changes in the pH level in the neonatal brain, APT-MT imaging can help to understand neonatal brain injury.
[Keywords] Magnetic resonance imaging；Neonatal；Brain；Amide proton transfer；Magnetization transfer imaging

ZHENG Yang Department of Radiology, Shengjing Hospital of China Medical University, Shenyang 110004, China

WANG Xiao-ming* Department of Radiology, Shengjing Hospital of China Medical University, Shenyang 110004, China

*Correspondence to: Wang XM, E-mail: wangxm024@163.com

Conflicts of interest   None.

ACKNOWLEDGMENTS  This study was supported by National Natural Science Foundation of China NO. 30570541, 30770632, 81271631
Received  2016-12-19
Accepted  2017-01-10
DOI: 10.12015/issn.1674-8034.2017.03.006
DOI:10.12015/issn.1674-8034.2017.03.006.

[1]
Liu Y, Jissendi-Tchofo P, Metens T. MR imaging, diffusion imaging, and proton MR spectroscopy at 3T in full-term neonates with hypoxic-ischemic encephalopathy. Chin J Magn Reson Imaging, 2011, 2(1): 13-18.
[2]
Malik GK, Pandey M, Kumar R, et al. MR imaging and in vivo proton spectroscopy of the brain in neonates with hypoxic ischemic encephalopathy. Eur J Radiol, 2002, 43(1): 6-13.
[3]
Khong PL, Tse C, Wong IY, et al. Diffusion-weighted imaging and proton magnetic resonance spectroscopy in perinatal hypoxic-ischemic encephalopathy: association with neuromotor outcome at 18 months of age. J Child Neurol, 2004, 19(11): 872-881.
[4]
Wang HW, Wang XM, Guo QY. The correlation between DTI parameters and levels of AQP-4 in the early phases of cerebral edema after hypoxic-ischemic/reperfusion injury in piglets. Pediatr Radiol, 2012, 42(8): 992-999.
[5]
Distefano G, Praticò AD. Actualities on molecular pathogenesis and repairing processes of cerebral damage in perinatal hypoxic-ischemic encephalopathy. Ital J Pediatr, 2010, 36: 63.
[6]
Uria-Avellanal C, Robertson NJ. Na+/H+ exchangers and intracellular pH in perinatal brain injury. Transl Stroke Res, 2014, 5(1): 79-98.
[7]
Hamakawa H, Murashita J, Yamada N, et al. Reduced intracellular pH in the basal ganglia and whole brain measured by 31P-MRS in bipolar disorder. Psychiatry Clin Neurosci, 2004, 58(1): 82-88.
[8]
Casey JR, Grinstein S, Orlowski J. Sensors and regulators of intracellular pH. Nat Rev Mol Cell Biol, 2010, 11(1): 50-61.
[9]
Schmidt H, Schwenzer NF, Gatidis S, et al. Systematic Evaluation of Amide Proton Chemical Exchange Saturation Transfer at 3 T: Effects of Protein Concentration, pH, and Acquisition Parameters. Invest Radiol, 2016, 51(10): 635-646.
[10]
Zhou J, Payen J, Wilson DA, et al. Using the amide proton signals of intracellular proteins and peptides to detect pH effects in MRI. Nat Med, 2003, 9(8): 1085-1090.
[11]
Yan K, Fu Z, Yang C, et al. Assessing amide proton transfer (APT) MRI contrast origins in 9 L gliosarcoma in the rat brain using proteomic analysis. Mol Imaging Biol, 2015, 17(4): 479-487.
[12]
Zhou J, Wilson DA, Sun PZ, et al. Quantitative description of proton exchange processes between water and endogenous and exogenous agents for WEX, CEST, and APT experiments. Magn Reson Med, 2004, 51(5): 945-952.
[13]
Jokivarsi KT, Grohn HI, Grohn OH, et al. Proton transfer ratio, lactate, and intracellular pH in acute cerebral ischemia. Magn Reson Med, 2007, 57(4): 647-653.
[14]
Togao O, Kessinger CW, Huang G, et al. Characterization of lung cancer by amide proton transfer (APT) imaging: an in-vivo study in an orthotopic mouse model. PLoS One, 2013, 8(10): e77019.
[15]
Dula Adrienne N, Arlinghaus Lori R, Dortch Richard D, et al. Amide proton transfer imaging of the breast at 3 T: establishing reproducibility and possible feasibility assessing chemotherapy response. Magn Reson Med, 2013, 70(1): 216-224.
[16]
Klomp Dennis WJ, Dula Adrienne N, Arlinghaus Lori R, et al. Amide proton transfer imaging of the human breast at 7 T: development and reproducibility. NMR Biomed, 2013, 26(10):1271-1277.
[17]
Jia G, Abaza R, Williams JD. Amide proton transfer MR imaging of prostate cancer: a preliminary study. J Magn Reson Imaging, 2011, 33(3): 647-654.
[18]
Gerigk L, Schmitt B, Stieltjes B. 7 Tesla imaging of cerebral radiation necrosis after arteriovenous malformations treatment using amide proton transfer (APT) imaging. J Magn Reson Imaging, 2012, 35(5): 1207-1209.
[19]
Sun PZ, Zhou J, Sun W, et al. Detection of the ischemic penumbra using pH-weighted MRI. J Cereb Blood Flow Metab, 2007, 27(6): 1129-1136.
[20]
Henkelman RM, Stanisz GJ, Graham SJ. Magnetization transfer in MRI: a review. NMR Biomed, 2001, 14(2): 57-64.
[21]
Wen Z, Hu S, Huang F, et al. MR imaging of high-grade brain tumors using endogenous protein and peptide-based contrast. Neuroimage, 2010, 51(2): 616-622.
[22]
Robertson NJ, Cowan FM, Cox IJ, et al. Brain alkaline intracellular pH after neonatal encephalopathy. Ann Neurol, 2002, 52(6): 732-742.
[23]
Kucharczyk W, Macdonald PM, Stanisz GJ, et al. Relaxation and magnetization transfer of white matter lipidsat MR imaging: importance of cerebtosides and pH. Radiology, 1994, 192(2): 521-529.
[24]
Zhou J. Amide proton transfer imaging of the human brain. Methods Mol Biol, 2011, 711: 227-237.
[25]
Barkovich AJ, Kjos BO, Jackson DE Jr, et al. Normal maturation of the neonatal and infant brain: MR imaging at 1.5 T. Radiology, 1988, 166(1pt 1): 173-180.
[26]
Oishi K, Mori S, Donohue PK, et al. Multi-contrast human neonatal brain atlas: application to normal neonate development analysis. Neuroimage, 2011, 56(1): 8-20.
[27]
Wang HW, Wang XM, Guo QY. The correlation between DTI parameters and levels of AQP-4 in the early phases of cerebral edema after hypoxic-ischemic reperfusion injury in piglets. Pediatric Radiology, 2012, 42(8): 992-999.
[28]
Malik GK, Pandey M, Kumar R, et al. MR imaging and in vivo proton spectroscopy of the brain in neonates with hypoxic ischemic encephalopathy. Eur J Radiol, 2002, 43(1): 6-13.
[29]
Fern R, Möller T. Rapid ischemic cell death in immature oligodendrocytes: a fatal glutamate release feedback loop. J Neurosci, 2000, 20(1): 34-42.
[30]
Matute C, Alberdi E, Domercq M. The link between excitotoxic oligodendroglial death and demyelinating diseases. Trends Neurosci, 2001, 24(4): 224-230.
[31]
Zhou J, Lal B, Wilson DA, et al. Amide proton transfer (APT) contrast for imaging of brain tumors. Magn Reson Med, 2003, 50(6): 1120-1126.
[32]
Sun PZ, Zhou J, Huang Judy, at el. Simplified quantitative description of amide proton transfer (APT) imaging during acute ischemia. Magn Reson Med, 2007, 57(2): 405-410.
[33]
Sun PZ, Wang E, Cheung JS. Imaging acute ischemic tissue acidosis with pH-sensitive endogenous amide proton transfer (APT) MRI: correction of tissue relaxation and concomitant RF irradiation effects toward mapping quantitative cerebral tissue PH. Neuroimage, 2012, 60(1): 1-6.
[34]
Yilmaz U. Diffusion-weighted imaging in acute stroke. Radiologe, 2015, 5(9): 771-774.
[35]
Liu D, Zhou J, Xue R, et al. Quantitative characterization of nuclear overhauser enhancement and amide proton transfer effects in the human brain at 7 tesla. Magn Reson Med, 2013, 70(4): 1070-1081
[36]
Zhou J, Payen J, van Zijl PC. The interaction between magnetization transfer and blood-oxygen level-dependent effects. Magn Reson Med, 2005, 53(2): 356-366.
[37]
Scheidegger R, Vinogradov E, Alsop DC. Amide proton transfer imaging with improved robustness to magnetic field inhomogeneity and magnetization transfer asymmetry using saturation with frequency alternating RF irradiation. Magn Reson Med, 2011, 66(5): 1275-1285.

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