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• Original Article •
MitoQ protects rodent kidneys from ischemia-reperfusion injury: observations with DWI
LIU Xiao-ge  WU Huan-huan  ZHANG Rui  SUN Hao-ran 

DOI:10.12015/issn.1674-8034.2017.07.009.


[Abstract] Objective: To investigate the effect of the mitochondria targeted antioxidant MitoQ in protecting from renal ischemia-reperfusion injury (IRI) in rats by longitudinal observation on apparent diffusion coefficient (ADC) values of the kidney measured with diffusion-weighted imaging (DWI).Materials and Methods: Renal IRI was induced by temporarily clamping the left renal artery for 45 minutes and then reperfusion was realized. Ten male Sprague-Dawley rats were randomly divided into two groups: MitoQ group (n=5, IRI+MitoQ) and control group (n=5, IRI+saline). DWI was performed just before the operation (day 0) and on day 2, 5, 7 and 14 after the operation. ADC value of the outer stripe of the outer medulla (OSOM) was measured on bilateral kidneys. Renal histopathology damage score was evaluated after the final MRI examination. Inter- and intra- group differences were assessed using the Least Significant Difference (LSD). Kruskal-Wallis H test and Mann-Whitney U test were used for histopathology damage score analysis.Results: The ADC values of the kidneys in two groups on day 0 had no statistical significance. The ADC values of the left OSOM dropped dramatically after IRI in both of the groups, and it was more obvious in control group. The ADC values of the OSOM on IRI kidneys were lower than their counterparts (P <0.01), and the ADC values of the OSOM in control group were even lower than that in MitoQ group Day 2, the left OSOM on MitoQ group and control group: (3.66±0.29)×10-4 mm2/s. (3.09±0.39)×10-4 mm2/s, P<0.05. Day 5, the left OSOM on MitoQ group and control group: (3.75±0.32)×10-4 mm2/s, (2.95±0.79)×10-4 mm2/s, P<0.05. Day 7, the left OSOM on MitoQ group and control group: (3.77±0.42)×10-4 mm2/s, (2.98±0.49)×10-4 mm2/s, P<0.05, Day 14, the left OSOM on MitoQ group and control group: (3.93±0.23)×10-4 mm2/s, (3.05±0.20)×10-4 mm2/s, P<0.05. Renal histopathology analysis showed that renal damage was the most predominant on the OSOM of IRI kidneys in control group, whose histopathology damage scores were significantly higher than those in MitoQ group (P<0.01).Conclusions: DWI could be a noninvasive method to evaluate the effect of MitoQ on reducing renal IRI in rats.
[Keywords] Kidney；Ischemia-reperfusion injury；Diffusion magnetic resonance imaging

LIU Xiao-ge Department of Radiology, Tianjin Medical University General Hospital, Tianjin 300052, China

WU Huan-huan Department of Radiology, Tianjin Medical University General Hospital, Tianjin 300052, China

ZHANG Rui Department of Radiology, Tianjin Medical University General Hospital, Tianjin 300052, China

SUN Hao-ran* Department of Radiology, Tianjin Medical University General Hospital, Tianjin 300052, China

*Correspondence to: Sun HR, E-mail: sunhaoran2006@hotmail.com

Conflicts of interest   None.

ACKNOWLEDGMENTS  This work was part of National Natural Science Foundation of China No. 81171316
Received  2017-03-07
Accepted  2017-06-07
DOI: 10.12015/issn.1674-8034.2017.07.009
DOI:10.12015/issn.1674-8034.2017.07.009.

[1]
Smith ZL. Current Status of Minimally Invasive Surgery for Renal Cell Carcinoma. Curr Urol Rep, 2016, 17(6): 43.
[2]
Schneider MJ, Dietrich O, Ingrisch M, et al. Intravoxel incoherent motion Magnetic Resonance Imaging in partially nephrectomized kidneys. Invest Radiol, 2016, 51(5): 323-330.
[3]
Thompson RH, Lane BR, Lohse CM, et al. Every minute counts when the renal hilum isclamped during partial nephrectomy. Eur Urol, 2010, 58(3): 340-345.
[4]
Kaouk JH, Malkoç E. Is robotic partial nephrectomy convenient for solitary kidney? Turk J Urol, 2016, 42(3): 127-129.
[5]
Hu L, Chen J, Yang X, et al. Assessing intrarenal nonperfusion and vascular leakage in acute kidney injury with multinuclear (1) H/(19) F MRI and perfluorocarbon nanoparticles. Magn Reson Med, 2014, 71(6): 2186-2196.
[6]
Ysebaert DK, De Greef KE, Vercauteren SR, et al. Identification and kinetics of leukocytes after severe ischaemia/reperfusion renal injury. Nephrol Dial Transplant, 2000, 15(10): 1562-1574.
[7]
Kalogeris T, Bao Y, Korthuis RJ. Mitochondrial reactive oxygen species: a double edged sword in ischemia/reperfusion vs preconditioning. Redox Biol, 2014, 2: 702-714.
[8]
Guan W, Wang Z, Liu Y, et al. Protective effects of tirofiban on ischemia/reperfusion-induced renal injury in vivo and in vitro. Eur J Pharmacol, 2015, 761: 144-152.
[9]
Oyewole AO, Birch-Machin MA. Mitochondria-targeted antioxidants. FASEB J, 2015, 29(12): 4766-4771.
[10]
Zhong H, Dai HY, Li LH, et al. Study of MRI enhancement and diffusion weighted imaging in the recurrence of nasopharyngeal carcinoma after radiotherapy. Chin J Magn Reson Imaging, 2016, 7(6): 433-437.钟华，代海洋，李丽红，等．MRI增强及DWI对鼻咽癌放疗后复发的诊断价值研究．磁共振成像，2016，7(6)：433-437．
[11]
Zhang YY, Zha YF, Li L, et al. Correlation between estimated glomerular fltration rate (eGFR) and apparent diffusion coeffcient (ADC) values of the kidneys. Chin J Magn Reson Imaging, 2016, 7(8): 567-571.张友元，查云飞，李亮，等．肾脏MR表观扩散系数与估算肾小球滤过率的关系．磁共振成像，2016，7(8)：567-571．
[12]
Jia RP, Xie JJ, Luo FY, et al. Ischemic preconditioning improves rat kidney allograft function after ischemia/reperfusion injury: the role of tumor necrosis factor-alpha. Transplant Proc, 2008, 40(10): 3316-3320.
[13]
Abreu Lde A, Kawano PR, Yamamoto H, et al. Comparative study between trimetazidine and ice slush hypothermia in protection against renal ischemia/reperfusion injury in a porcine model. Int Braz J Urol, 2011, 37(5): 649-656.
[14]
Eltzschig HK, Eckle T. Ischemia and reperfusion--from mechanism to translation. Nat Med, 2011, 17(11): 1391-1401.
[15]
Dare AJ, Bolton EA, Pettigrew GJ, et al. Protection against renal ischemia-reperfusion injury in vivo by the mitochondria targeted antioxidant MitoQ. Redox Biol, 2015, 5: 163-168.
[16]
Nielsen PM, Szocska Hansen ES, Nørlinger TS, et al. Renal ischemia and reperfusion assessment with three-dimensional hyperpolarized 13 C, 15 N2-urea. Magn Reson Med, 2016, 76(5): 1524-1530.
[17]
Zhang JL, Rusinek H, Chandarana H, et al. Functional MRI of the kidneys. J Magn Reson Imaging, 2013, 37(2): 282-293.
[18]
Sari-Sarraf F, Pomposiello S, Laurent D. Acute impairment of rat renal function by L-NAME as measured using dynamic MRI. MAGMA, 2008, 21(4): 291-297.
[19]
Chen B, Zhang Y, Song X, et al. Quantitative estimation of renal function with dynamic contrast-enhanced MRI using a modified two-compartment model. PLoS One, 2014, 9(8): e105087.
[20]
Thoeny HC, De Keyzer F. Diffusion-weighted MR imaging of native and transplanted kidneys. Radiology, 2011, 259(1): 25-38.
[21]
Togao O, Doi S, Kuro-o M, et al. Assessment of renal fibrosis with diffusion weighted MR imaging: study with murine model of unilateralureteral obstruction. Radiology, 2010, 255(3): 772-780.
[22]
Thoeny HC, De Keyzer F. Diffusion-weighted MR imaging of native and transplanted kidneys. Radiology, 2011, 259(1): 25-38.
[23]
Thoeny HC, Grenier N. Science to practice: can diffusion-weighted MR imaging findings be used as biomarkers to monitor the progression of renal fibrosis? Radiology, 2010, 255(3): 667-668.
[24]
Tao JY, Mi H, Mo ZN, et al. Protective effect of curcumin preconditioning and ischemic postconditioning against renal ischemia-reperfusion injury in rats. Chin J Tissue Engin Res, 2012, 16(53): 10015-10020.陶佳意，米华，莫曾南，等．姜黄素预处理及缺血后处理对大鼠肾脏缺血再灌注损伤的保护作用．中国组织工程研究，2012，16(53)：10015-10020．
[25]
Yang HX, Jiang ZX, Yu SN, et al. Assessment of glomerular filtration rate using diffusion weighted imaging: a comparative study of IVIM and mono-exponential models. Chin J Magn Reson Imaging, 2016, 7(9): 679-682.杨荷霞，蒋振兴，俞胜男，等．肾脏DWI的IVIM及单指数模型预测肾小球滤过率的比较研究．磁共振成像，2016，7(9)：679-682．
[26]
Wu HH, Jia HR, Zhang Y, et al. Monitoring the progression of renal fibrosis by T2-weighted signal intensity and diffusion weighted magnetic resonance imaging in cisplatin induced rat models. Chin Med J (Engl), 2015, 128(5): 626-631.

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