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• Original Article •
Preparation of folate-mediated superparamagnetic iron oxide-dopamine-folate and its in vitro MR imaging
TANG Na  ZHAO Guang-ming  LI Zheng-yu  ZHANG Gui-xiang  WANG Zhong-ling 

DOI:10.12015/issn.1674-8034.2017.08.009.


[Abstract] Objective: To observe the targeting function of folate-conjugated superparamagnetic iron oxide-dopamine (NP-DA-Dextra-FA) to Bel7402 cells by folate receptor (FR) and the possibility for monitoring tumor targeting with MRI.Materials and Methods: NP-DA-Dextra-FA and NP-DA-Dextran were hatched with Bel7402 cells for 2 h, separately. Prussian blue staining was used to survey intracellular irons, meanwhile MRI was used to research the signal intensity of cells. The competitive inhibition test was performed.Results: The experimental group showed the T2WI signal intensity of Bel7402 cells declined significantly with the rise of NP-DA-Dextra-FA’s iron concentration. A lot of intracellular irons were observed in Bel7402 cells. The control group showed the signal intensity of Bel7402 cells on T2WI decreased less clearly. Some intracellular irons were showed in Bel7402 cells. The competitive inhibition test showed the T2WI signal intensity increased with the folic acid concentration rising.Conclusion: In vitro, the NP-DA-Dextra-FA has obvious targeting ability to Bel7402 cells. The cell targeting processes could be supervised with MRI.
[Keywords] Carcinoma, hepatocellular；Folic acid；Targeting imaging；Magnetic resonance imaging；Molecular probes

TANG Na Department of Radiology, The First People's Hospital of Shanghai, Shanghai 200085, China

ZHAO Guang-ming Department of Radiology, The First People's Hospital of Shanghai, Shanghai 200085, China

LI Zheng-yu Department of Radiology, The First People's Hospital of Shanghai, Shanghai 200085, China

ZHANG Gui-xiang Department of Radiology, The First People's Hospital of Shanghai, Shanghai 200085, China

WANG Zhong-ling* Department of Radiology, The First People's Hospital of Shanghai, Shanghai 200085, China

*Correspondence to: Wang ZL, E-mail: zlwang138136@126.com

Conflicts of interest   None.

ACKNOWLEDGMENTS  This work was part of National Natural Science Foundation of China No. 81371623
Received  2017-02-04
Accepted  2017-06-12
DOI: 10.12015/issn.1674-8034.2017.08.009
DOI:10.12015/issn.1674-8034.2017.08.009.

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