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• Original Article •
Effects of different concentrations of magnetic probe on growth of adipose stem cells and T2*mapping imaging
XIE Guang-you  GENG Tao  YANG Hai-tao  LÜ Fu-rong  ZENG Xian-chun  LIU Chang-jie  YANG Ming-fang  WANG Rong-pin 

DOI:10.12015/issn.1674-8034.2017.09.007.


[Abstract] Objective: To study the safe concentration of superparamagnetic iron oxide particles with poly-l-lysine (SPIO-PLL) labelling rabbit adipose stem cells (ADSCs) and quantative T2*mapping imaging.Materials and Methods: The cells labelled with 25, 50, 75 μg/ml SPIO-PLL were detected by Prussian blue staining and transmission electron microscopy (TEM). The cell cycle and apoptosis were analysed by flow cytometry (FCM). 1×106 cells unlabelled and 1×106 cells (labelled 1 d), 1×106 (labelled 3 d) and 5×105 (labelled 1 d) with 25 μg/ml SPIO-PLL were performed with GRE T2*WI and T2*mapping sequences scanning, then the relaxation time of each tube were measured.Results: The results of Prussian blue staining showed the labelled rate was nearly 100%, and TEM displayed particles existed in every cytoplasm. The blocking rate of cell cycle and the rate of cell apoptosis of cells labelled with 25 μg/ml SPIO-PLL had no significant difference compared with the blank group (P>0.05), 25 μg/ml SPIO-PLL was the safe concentration. The signal intensity (SI) of 1×106 cells (labelled 1 d) was the lowest. The T2* relaxation time of each labelled groups had significant difference compared with the blank group (F=169.837, P<0.01).Conclusions: ADSCs can be labelled safely and efficiently by 25 μg/ml SPIO-PLL and cell population can be scanned by MR. T2*mapping can be used to quantatively monitor the relaxation time of labelled cells.
[Keywords] Adipose derived stem cells；Rabbits；Magnetic probe；Magnetic resonance imaging

XIE Guang-you Department of Radiology, Guizhou Provincial People's Hospital, Guiyang, 550002, China

GENG Tao Department of Oncology, Guizhou Provincial People’s Hospital, Guiyang 550002, China

YANG Hai-tao* Department of Radiology, The First Affiliated Hospital of Chongqing Medical University, Chongqing 400016, China

LÜ Fu-rong* Department of Radiology, The First Affiliated Hospital of Chongqing Medical University, Chongqing 400016, China

ZENG Xian-chun Department of Radiology, Guizhou Provincial People's Hospital, Guiyang, 550002, China

LIU Chang-jie Department of Radiology, Guizhou Provincial People's Hospital, Guiyang, 550002, China

YANG Ming-fang Department of Radiology, Guizhou Provincial People's Hospital, Guiyang, 550002, China

WANG Rong-pin Department of Radiology, Guizhou Provincial People's Hospital, Guiyang, 550002, China

*Correspondence to: Yang HT, E-mail: frankyang119@126.com. Lü FR, E-mail: lfr918@sina.com

Conflicts of interest   None.

ACKNOWLEDGMENTS  The paper was Supported by the Natural Science Funding of Chongqing No. cstc2011jjA10082 National Clinical Key Subject Construction Project No. 2013-544
Received  2017-05-07
Accepted  2017-08-10
DOI: 10.12015/issn.1674-8034.2017.09.007
DOI:10.12015/issn.1674-8034.2017.09.007.

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