Share：
Share this content in WeChat
X
[Chinese][PDF]103775
• Original Article •
A longitudinal study of changes of brain metabolites in vascular cognitive impairment rats using 3.0 T 1H-MRS
HUANG Ming-ming  CAO Xiao-wan  SHEN Gui-quan  LI Xiao-bao  YU Hui 

DOI:10.12015/issn.1674-8034.2017.09.009.


[Abstract] Objective: To explore the application of 3.0 T 1H-MRS in assessment of longitudinal changes of brain metabolites in vascular cognitive impairment (VCI) rats and its potential value in early diagnosis of VCI disease.Materials and Methods: Philips 3.0 T MR scanner combined with animal coil were applied to obtain 1H-MRS data of brain (mainly including the hippocampus) in VCI rats at different time points (2 weeks, 1 month, 3 months and 5 months), Morris Water maze test and pathologic examination (HE and Nissle staining) were also performed to evaluate the cognitive impairment and pathological changes.Results: Compared with controls, at 2 weeks, significantly cognitive impairment and deduction of Glu/tCr ratio (P<0.05)were found. And reduction of NAA/tCr (P<0.05) and increasing of mI/tCr (P<0.05) were detected at 1 month, at 3 months, the increase of mI/tCr were kept, and also GPC/tCr (P<0.05) and tCho/tCr (P<0.05) were raised, at 5 months, all metabolites recovered to normal level besides mI/tCr. But, HE and Nissle stainings showed that cell structural and morphological changes can also exist in the hippocampus.Conclusions: The abnormal metabolites provided by 3.0 T 1H-MRS maybe can reflect the pathological changes in progression of VCI, objectively. Results from this study suggested that reduction of Glu/tCr at the onset and sustained rise of mI/tCr in the progress of VCI, can be helpful for diagnosis of VCI at its early stage.
[Keywords] Cognition disorders；Blood vessels；Hippocampus；Proton magnetic resonance spectroscopy

HUANG Ming-ming Department of Radiology, Affiliated Hospital of Guizhou Medical University, Guiyang 550004, China

CAO Xiao-wan Department of Radiology, Affiliated Hospital of Guizhou Medical University, Guiyang 550004, China

SHEN Gui-quan Department of Radiology, Affiliated Hospital of Guizhou Medical University, Guiyang 550004, China

LI Xiao-bao Department of Radiology, Affiliated Hospital of Guizhou Medical University, Guiyang 550004, China

YU Hui* Department of Radiology, Affiliated Hospital of Guizhou Medical University, Guiyang 550004, China

*Correspondence to: Yu H, E-mail: 331693861@qq.com

Conflicts of interest   None.

ACKNOWLEDGMENTS  This work is supported by grants from the Natural Science Foundation of China No. 8156070059 Science and Technology Department of Guizhou Province No. Qian-ke-he LG 2012-024, TN2014-51 Higher Education Research Center of Guizhou Province No. Qian-jiao-he KY 2016-012
Received  2017-04-14
Accepted  2017-07-20
DOI: 10.12015/issn.1674-8034.2017.09.009
DOI:10.12015/issn.1674-8034.2017.09.009.

[1]
Hachinski VC, Lassen NA, Marshall J. Multi-infarct dementia. A cause of mental deterioration in the elderly. Lancet (London, England), 1974, 2(7874): 207-210.
[2]
Hachinski V. Preventable senility: a call for action against the vascular dementias. Lancet (London, England), 1992, 340(8820):645-648.
[3]
Wardlaw JM, Smith EE, Biessels GJ, et al. Neuroimaging standards for research into small vessel disease and its contribution to ageing and neurodegeneration. Lancet Neurol, 2013, 12(8): 822-838.
[4]
Pulsinelli WA, Brierley JB. A new model of bilateral hemispheric ischemia in the unanesthetized rat. Stroke, 1979, 10(3): 267-272.
[5]
Ramin SL, Tognola WA, Spotti AR. Proton magnetic resonance spectroscopy: clinical applications in patients with brain lesions. Sao Paulo Med J, 2003, 121(6): 254-259.
[6]
Mayer D, Zahr NM, Sullivan EV, et.al. In vivo metabolite differences between the basal ganglia and cerebellum of the rat brain detected with proton MRS at 3 T. Psychiatry Res, 2007, 154(3): 267-273.
[7]
Aradi M, Steier R, Bukovics P, et al. Quantitative proton mri and mrs of the rat brain with a 3 T clinical mr scanner. J Neuroradiol, 2011, 38(2): 90-97.
[8]
Bleich S, Romer K, Wiltfang J, et al. Glutamate and the glutamate receptor system: a target for drug action. Int J Geriatr Psychiatry, 2003, 18(Suppl 1): S33-40.
[9]
Rupsingh R, Borrie M, Smith M, et al. Reduced hippocampal glutamate in Alzheimer disease. Neurobiol Aging, 2011, 32(5):802-810.
[10]
Westhoff TH, Schubert F, Wirth C, et al. The impact of blood pressure on hippocampal glutamate and mnestic function. J Hum Hypertens, 2011, 25(4): 256-261.
[11]
Shiino A, Watanabe T, Shirakashi Y, et al. The profile of hippocampal metabolites differs between Alzheimer's disease and subcortical ischemic vascular dementia, as measured by proton magnetic resonance spectroscopy. J Cereb Blood Flow Metab, 2012, 32(5):805-815.
[12]
Sun D, Zhang J, Fan Y, et al. Abnormal levels of brain metabolites may mediate cognitive impairment in stroke-free patients with cerebrovascular risk factors. Age Ageing, 2014, 43(5): 681-686.
[13]
Zhang H, Huang M, Gao L, et al. Region-specific cerebral metabolic alterations in streptozotocin-induced type 1 diabetic rats: an in vivo proton magnetic resonance spectroscopy study. J Cereb Blood Flow Metab, 2015, 35(11): 1738-1745.
[14]
Fisher SK, Novak JE, Agranoff B W. Inositol and higher inositol phosphates in neural tissues: homeostasis, metabolism and functional significance. J Neurochem, 2002, 82(4): 736-754.
[15]
Yu AC, Wong HK, Yung HW, et al. Ischemia-induced apoptosis in primary cultures of astrocytes. Glia, 2001, 35(2): 121-130.
[16]
张波，闻彩云，王玲,等.皮层下缺血患者认知功能评估及功能磁共振研究.中华内科杂志, 2011, 50(5): 411-415.
[17]
Chen SQ, Cai Q, Shen YY, et al. Hydrogen proton magnetic resonance spectroscopy in multidomain amnestic mild cognitive impairment and vascular cognitive impairment without dementia. Am J Alzheimers Dis Other Demen, 2016, 31(5): 422-429.
[18]
楼海燕，漆剑频，张敏鸣,等.血管性认知功能障碍海马的磁共振波谱研究.中华医学杂志, 2006, 86(34): 2428-2430.
[19]
Herminghaus S, Frolich L, Gorriz C, et al. Brain metabolism in Alzheimer disease and vascular dementia assessed by in vivo proton magnetic resonance spectroscopy. Psychiatry Res, 2003, 123(3): 183-190.
[20]
Meng N, Shi S, Su Y. Proton magnetic resonance spectroscopy as a diagnostic biomarker in mild cognitive impairment following stroke in acute phase. 2016, Neuroreport, 27(8): 559-563.
[21]
Mackay S, Meyerhoff DJ, Constans JM, et al. Regional gray and white matter metabolite differences in subjects with AD, with subcortical ischemic vascular dementia, and elderly controls with 1H magnetic resonance spectroscopic imaging. Arch Neurol, 1996,53(2): 167-174.
[22]
Suriyajakryuththana W, Tuntiyatorn L, Teepprasarn N, et al. Proton magnetic resonance spectroscopy in mild cognitive impairment and Alzheimer's disease: a preliminary study. J Med Assoc Thai, 2014,97(4): 407-414.

PREV MR diffusion kurtosis imaging versus standard diffusion imaging: changes with radiation in uterine cervical carcinoma xenografts
NEXT Anaphylactic shock caused by Ga-dobenate dimeglumine was in timely treated successfully: a case report
  


LINK


Tel & Fax: +8610-67113815    E-mail: editor@cjmri.cn