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• Clinical Article •
The effect of leucine on feeding center and reward system in T2DM patients and healthy control subjects: a resting-state fMRI study
LI Mei-jiao  LIU Hua-sheng  GU Qian-biao  DENG Ling-ling  DUAN Jun-hong  LIANG Qi  RONG Peng-fei  WANG Wei 

DOI:10.12015/issn.1674-8034.2018.07.002.


[Abstract] Objective: To study the functional connectivity of the reward system and feeding center after oral leucine in type 2 diabetes mellitus by resting-state fMRI, and analyze the relationships between the changes and related metabolic indicators of T2DM during this test.Materials and Methods: This study included several T2DM patients (T2DM, n=9) whose age, sex, education match up with healthy control subjects (HC, n=8). Resting-state fMRI data were collected by blood oxygen level dependent sequence (BOLD). Brain regions involving in feeding center and reward system were selected as seeds, and functional connection values between these seeds and other voxels in the brain were calculated after oral leucine, the resulted values of both groups were compared using two-sample t-test to locate the regions with significant change. Then correlation analysis was conducted between clinical indexes and values of functional connection extracted from significant difference between groups.Results: After oral leucine, HC group people showed significantly increased functional connectivity between left orbitofrontal cortex and right cerebellum posterior lobe (P<0.05). The functional connection values between the prefrontal cortex, the anterior cingulum cortex, orbitofrontal cortex, limbic lobe and left putamen increased significantly (P<0.05), however there were no significant differences in T2DM group (P>0.05). Moreover, negative correlations were found between the changes of functional connection values of brain function and fasting serum insulin, HOMA2-IR in T2DM group (P< 0.05). Positively correlations were found between the changes of functional connection values of brain function and plasma leucine level in HC group (P<0.05).Conclusions: T2DM damage the functional connectivity of reward-associated brain regions, and the functional connectivity between left OFC and cerebellar tonsils was correlates with the degree of HOMA2-IR and fasting serum insulin. Moreover, this study indicated that central insulin resistance may damage the leucine sensing pathway.
[Keywords] Diabetes mellitus, Type 2；Leucine；Magnetic resonance imaging；Reward；Feeding center

LI Mei-jiao Department of Radiology, the Third Xiangya Hospital of Central South University, Changsha 410013, China

LIU Hua-sheng Department of Radiology, the Third Xiangya Hospital of Central South University, Changsha 410013, China

GU Qian-biao Department of Radiology, the Third Xiangya Hospital of Central South University, Changsha 410013, China

DENG Ling-ling Department of Radiology, the Third Xiangya Hospital of Central South University, Changsha 410013, China

DUAN Jun-hong Department of Radiology, the Third Xiangya Hospital of Central South University, Changsha 410013, China

LIANG Qi Department of Radiology, the Third Xiangya Hospital of Central South University, Changsha 410013, China

RONG Peng-fei Department of Radiology, the Third Xiangya Hospital of Central South University, Changsha 410013, China

WANG Wei* Department of Radiology, the Third Xiangya Hospital of Central South University, Changsha 410013, China

*Correspondence to: Wang W, E-mail: cjr.wangwei@vip.163.com

Conflicts of interest   None.

ACKNOWLEDGMENTS  This work was part of project of National Key Clinical Specialty No. 2013-544 National Natural Science Foundation of China No. 81471715
Received  2018-03-07
Accepted  2018-05-15
DOI: 10.12015/issn.1674-8034.2018.07.002
DOI:10.12015/issn.1674-8034.2018.07.002.

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