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• Original Article •
The study of susceptibility weighted imaging of amyloid plaques and iron deposits in the brain of APP/PS1 transgenic AD mice
LIU Hao-di  ZHENG Yang  WANG Xiao-ming 

DOI:10.12015/issn.1674-8034.2018.08.010.


[Abstract] Objective: The aim is to apply the three dimensional enhanced magnetic sensitive imaging (3D-enhanced susceptibility weighted angiography, ESWAN) technology to detect APPswe/PSEN1de9 gene mutant mice (purchased from the United States Jackson that referred to as APP/PS1 transgenic mice) average phase values from different parts of the brain (mean phase value, MPV), and to explore its and ferritin and correlation of the distribution of amyloid plaques.Materials and Methods: APP/PS1 mutant mice and wild-type control mice underwent T1WI, T2WI and ESWAN sequence scanning to measure MPV in the cerebral cortex, hippocampus and thalamus of mice. Immunohistochemical staining of iron protein and Aβ protein in rat brain tissues was carried out, and the percentage of the number and area of stained plaques in the area of interest were counted respectively. Pearson correlation was used to analyze the MPV, patch number and patch area percentage in the ROI region.Results: The MPV values in the cortex area (t=-2.201, P=0.045) and hippocampal area (t=-2.524, P=0.024) of both APP/PS1 dual-transgenic mice and control mice were statistically different. There was no statistically significant difference in MPV between APP/PS1 dual transgenic mice and control mice in thalamus region (t=-2.094, P=0.055). The number and range of iron protein plaques and MPV in each group of APP/PS1 dual-transgenic mice were significantly correlated (P<0.05). The amount and range of amyloid plaques and ferritin were correlated (P<0.05).Conclusions: The mean phase value in the brain of transgenic mice with APP/PS1 was significantly correlated with iron deposition in the brain. The iron deposition in the cerebral cortex, hippocampus and thalamus continued in the APP/PS1 dual transgenic mice, and was associated with the formation of amyloid plaques.
[Keywords] 3D-enhanced susceptibility weighted angiography；Mean phase value；Mice, transgenic；Amyloid plaques；Ferritin；Magnetic resonance imaging

LIU Hao-di Department of Radiology, Shengjing Hospital of China Medical University, Shenyang 110004, China

ZHENG Yang Department of Radiology, Shengjing Hospital of China Medical University, Shenyang 110004, China

WANG Xiao-ming* Department of Radiology, Shengjing Hospital of China Medical University, Shenyang 110004, China

*Correspondence to: Wang XM, E-mail: wangxm024@163.com

Conflicts of interest   None.

ACKNOWLEDGMENTS  This work was part of National Natural Science Foundation of China No. 81471720 Subsidized Project of Liaoning Provincial Education Department No. LZ2014039 and Outstanding Scientific Fund of Shengjing Hospital No. 201402
Received  2018-03-13
Accepted  2018-06-08
DOI: 10.12015/issn.1674-8034.2018.08.010
DOI:10.12015/issn.1674-8034.2018.08.010.

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