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• Original Article •
Detection of the drug resistance of human colon cancer in mice with proton magnetic resonance spectroscopy
XIE Qi  CHEN Huixian  DU Lei  LIAO Yanhui  TAN Zhilin  YANG Yiming  WU Minyi  PAN Qifeng  WU Jinbin  ZHANG Dingxuan 

Cite this article as: Xie Q, Chen HX, Du L, et al. Detection of the drug resistance of human colon cancer in mice with proton magnetic resonance spectroscopy. Chin J Magn Reson Imaging, 2019, 10(6): 435-439. DOI:10.12015/issn.1674-8034.2019.06.008.


[Abstract] Objective: To explore the possible molecular markers for evaluating the resistance of colon cancer with proton magnetic resonance spectroscopy (1H-MRS) in vivo.Materials and Methods: 8 nude mice with drug- response human colon cancer SW480 and 8 nude mice with drug-resistant human colon cancer SW480/5-FU were given 1H-MRS examinations when the maximum diameters of tumor were over 1.5 centimeters. Then these mice models were sacrificed with an overdose of anesthetic. Their tumors tissue were resected and detected the cell morphology and necrosis (HE stain) and protein expression of P53, P-gp, MPR1, PKC, γ-GCSh, γ-GCSl, GSHS, GST (Western blot).Results: The area of Cho, Lac, Glx and Ins/Cr of tumors in SW480/5-FU group were significantly higher than that in SW480 group (P<0.05). In HE section of tumor, there were small area necrosis in drug-resistant group and drug-response group, and no significant differences between the two groups. The intercellular space of tumor in drug-response group were larger than that of drug-resistant group. The expression of protein P-gp, MPR1, PKC, γ-GCSh, γ-GCSl, GSHS, GST in drug-resistant group were higher than that of drug-response group, and the expression of protein were statistically significant (P<0.05).Conclusions: The area of Cho, Lac, Glx1, Glx2, and Ins/Cr can be biomarkers to differentiate SW480/5-FU xenografts from SW480 xenografts in vivo.
[Keywords] magnetic resonance spectroscopy；magnetic resonance imaging；colonic neoplasms；xenograft model in nude mice；multiple drug resistance；animal experimentation

XIE Qi* Department of Imaging, Nansha Hospital, Guangzhou First People’s Hospital, Guangzhou Medical University, Guangzhou 510180, China; Department of Imaging, Nansha Hospital, Guangzhou First People’s Hospital, the Second Affiliated Hospital of South China University of Technology, Guangzhou 510180, China

CHEN Huixian Department of Imaging, Nansha Hospital, Guangzhou First People’s Hospital, Guangzhou Medical University, Guangzhou 510180, China

DU Lei Department of Imaging, Nansha Hospital, Guangzhou First People’s Hospital, Guangzhou Medical University, Guangzhou 510180, China

LIAO Yanhui Department of Imaging, Nansha Hospital, Guangzhou First People’s Hospital, Guangzhou Medical University, Guangzhou 510180, China

TAN Zhilin Department of Imaging, Nansha Hospital, Guangzhou First People’s Hospital, the Second Affiliated Hospital of South China University of Technology, Guangzhou 510180, China

YANG Yiming Guangdong Province Traditional Chinese Medical Hospital, Guangzhou 510030, China

WU Minyi Guangzhou Panyu Central Hospital, Guangzhou 511400, China

PAN Qifeng Department of Imaging, Nansha Hospital, Guangzhou First People’s Hospital, Guangzhou Medical University, Guangzhou 510180, China; Department of Imaging, Nansha Hospital, Guangzhou First People’s Hospital, the Second Affiliated Hospital of South China University of Technology, Guangzhou 510180, China

WU Jinbin Department of Imaging, Nansha Hospital, Guangzhou First People’s Hospital, Guangzhou Medical University, Guangzhou 510180, China; Department of Imaging, Nansha Hospital, Guangzhou First People’s Hospital, the Second Affiliated Hospital of South China University of Technology, Guangzhou 510180, China

ZHANG Dingxuan Department of Imaging, Nansha Hospital, Guangzhou First People’s Hospital, Guangzhou Medical University, Guangzhou 510180, China; Department of Imaging, Nansha Hospital, Guangzhou First People’s Hospital, the Second Affiliated Hospital of South China University of Technology, Guangzhou 510180, China

*Correspondence to: Xie Q, E-mail: xieqi8@21cn.com

Conflicts of interest   None.

ACKNOWLEDGMENTS  This work was part of Natural Science Foundation of Guangdong Province, Applied Basic Research Fund Project No.2015A030313732
Received  2019-01-12
Accepted  2019-05-17
DOI: 10.12015/issn.1674-8034.2019.06.008
Cite this article as: Xie Q, Chen HX, Du L, et al. Detection of the drug resistance of human colon cancer in mice with proton magnetic resonance spectroscopy. Chin J Magn Reson Imaging, 2019, 10(6): 435-439. DOI:10.12015/issn.1674-8034.2019.06.008.

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