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• Original Article •
Detection of the drug resistance of human colon cancer in mice with proton magnetic resonance spectroscopy
XIE Qi  CHEN Huixian  DU Lei  LIAO Yanhui  TAN Zhilin  YANG Yiming  WU Minyi  PAN Qifeng  WU Jinbin  ZHANG Dingxuan 

Cite this article as: Xie Q, Chen HX, Du L, et al. Detection of the drug resistance of human colon cancer in mice with proton magnetic resonance spectroscopy. Chin J Magn Reson Imaging, 2019, 10(6): 435-439. DOI:10.12015/issn.1674-8034.2019.06.008.


[Abstract] Objective: To explore the possible molecular markers for evaluating the resistance of colon cancer with proton magnetic resonance spectroscopy (1H-MRS) in vivo.Materials and Methods: 8 nude mice with drug- response human colon cancer SW480 and 8 nude mice with drug-resistant human colon cancer SW480/5-FU were given 1H-MRS examinations when the maximum diameters of tumor were over 1.5 centimeters. Then these mice models were sacrificed with an overdose of anesthetic. Their tumors tissue were resected and detected the cell morphology and necrosis (HE stain) and protein expression of P53, P-gp, MPR1, PKC, γ-GCSh, γ-GCSl, GSHS, GST (Western blot).Results: The area of Cho, Lac, Glx and Ins/Cr of tumors in SW480/5-FU group were significantly higher than that in SW480 group (P<0.05). In HE section of tumor, there were small area necrosis in drug-resistant group and drug-response group, and no significant differences between the two groups. The intercellular space of tumor in drug-response group were larger than that of drug-resistant group. The expression of protein P-gp, MPR1, PKC, γ-GCSh, γ-GCSl, GSHS, GST in drug-resistant group were higher than that of drug-response group, and the expression of protein were statistically significant (P<0.05).Conclusions: The area of Cho, Lac, Glx1, Glx2, and Ins/Cr can be biomarkers to differentiate SW480/5-FU xenografts from SW480 xenografts in vivo.
[Keywords] magnetic resonance spectroscopy；magnetic resonance imaging；colonic neoplasms；xenograft model in nude mice；multiple drug resistance；animal experimentation

XIE Qi* Department of Imaging, Nansha Hospital, Guangzhou First People’s Hospital, Guangzhou Medical University, Guangzhou 510180, China; Department of Imaging, Nansha Hospital, Guangzhou First People’s Hospital, the Second Affiliated Hospital of South China University of Technology, Guangzhou 510180, China

CHEN Huixian Department of Imaging, Nansha Hospital, Guangzhou First People’s Hospital, Guangzhou Medical University, Guangzhou 510180, China

DU Lei Department of Imaging, Nansha Hospital, Guangzhou First People’s Hospital, Guangzhou Medical University, Guangzhou 510180, China

LIAO Yanhui Department of Imaging, Nansha Hospital, Guangzhou First People’s Hospital, Guangzhou Medical University, Guangzhou 510180, China

TAN Zhilin Department of Imaging, Nansha Hospital, Guangzhou First People’s Hospital, the Second Affiliated Hospital of South China University of Technology, Guangzhou 510180, China

YANG Yiming Guangdong Province Traditional Chinese Medical Hospital, Guangzhou 510030, China

WU Minyi Guangzhou Panyu Central Hospital, Guangzhou 511400, China

PAN Qifeng Department of Imaging, Nansha Hospital, Guangzhou First People’s Hospital, Guangzhou Medical University, Guangzhou 510180, China; Department of Imaging, Nansha Hospital, Guangzhou First People’s Hospital, the Second Affiliated Hospital of South China University of Technology, Guangzhou 510180, China

WU Jinbin Department of Imaging, Nansha Hospital, Guangzhou First People’s Hospital, Guangzhou Medical University, Guangzhou 510180, China; Department of Imaging, Nansha Hospital, Guangzhou First People’s Hospital, the Second Affiliated Hospital of South China University of Technology, Guangzhou 510180, China

ZHANG Dingxuan Department of Imaging, Nansha Hospital, Guangzhou First People’s Hospital, Guangzhou Medical University, Guangzhou 510180, China; Department of Imaging, Nansha Hospital, Guangzhou First People’s Hospital, the Second Affiliated Hospital of South China University of Technology, Guangzhou 510180, China

*Correspondence to: Xie Q, E-mail: xieqi8@21cn.com

Conflicts of interest   None.

ACKNOWLEDGMENTS  This work was part of Natural Science Foundation of Guangdong Province, Applied Basic Research Fund Project No.2015A030313732
Received  2019-01-12
Accepted  2019-05-17
DOI: 10.12015/issn.1674-8034.2019.06.008
Cite this article as: Xie Q, Chen HX, Du L, et al. Detection of the drug resistance of human colon cancer in mice with proton magnetic resonance spectroscopy. Chin J Magn Reson Imaging, 2019, 10(6): 435-439. DOI:10.12015/issn.1674-8034.2019.06.008.

[1]
杨逸铭，谢琦. 体素内不相干运动扩散加权成像与氢质子磁共振频谱活体检测结肠癌耐药性及其生物标记物. 广州: 广州医科大学, 2017.
[2]
Mansoori B, Mohammadi A, Davudian S, et al.The different mechanisms of cancer drug resistance: A brief review. Adv Pharm Bull, 2017, 7(3): 339-348.
[3]
Junttila MR, de Sauvage FJ. Influence of tumour micro-environment heterogeneity on therapeutic response. Nature, 2013, 501(7467): 346-354.
[4]
Wu Q, Yang Z, Nie Y, et al. Multi-drug resistance in cancer chemotherapeutics: mechanisms and lab approaches. Cancer Lett, 2014, 347(2): 159-166.
[5]
王杰钦，游辅宇，彭青, 等. 肿瘤药敏试验的最新研究进展. 肿瘤, 2018, 38(9): 894-900.
[6]
Shah N, Sattar A, Benanti M, et al. Magnetic resonance spectroscopy as an imaging tool for cancer: a review of the literature. J Am Osteopath Assoc, 2006, 106(1): 23-27.
[7]
Imamura K. Proton MR spectroscopy of the brain with a focus on chemical issues. Magn Reson Med Sci, 2003, 2(3): 117-132.
[8]
Garcia-Figueiras R, Baleato-Gonzalez S, Padhani AR, et al. Proton magnetic resonance spectroscopy in oncology: the fingerprints of cancer?. Diagn Inter Radiol, 2016, 22(1): 75-89.
[9]
Sjobakk TE, Johansen R, Bathen TF, et al. Metabolic profiling of human brain metastases using in vivo proton MR spectroscopy at 3T. BMC Cancer, 2007, 7: 141.
[10]
Thews O, Gassner B, Kelleher DK, et al. Impact of extracellular acidity on the activity of P-glycoprotein and the cytotoxicity of chemotherapeutic drugs. Neoplasia, 2006, 8(2): 143-152.
[11]
易美芝，延根，张桂珊, 等. 用7.0 T 1H MR波谱对脑胶质瘤不同部位脑代谢特征的实验研究. 磁共振成像, 2014, 5(3): 206-210.
[12]
Uhm JH, Dooley NP, Villemure JG, et al. Glioma invasion in vitro: regulation by matrix metalloprotease-2 and protein kinase C. Clin Exp Metastasis, 1996, 14(5): 421-433.
[13]
李茹恬，钱晓萍，刘宝瑞. 恶性肿瘤pH依赖性生理性耐药及其对策.肿瘤, 2012, 32(5): 384-388.
[14]
Rae CD, Williams SR. Glutathione in the human brain: Review of its roles and measurement by magnetic resonance spectroscopy. Anal Biochem, 2017, 529: 127-143.
[15]
Traverso N, Ricciarelli R, Nitti M, et al. Role of glutathione in cancer progression and chemoresistance. Oxid Med Cell Longev, 2013, 2013: 972913.

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