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• Original Article •
Quantitative DCE-MRI and QCT were used to evaluate the bone marrow microvascular permeability and trabecular changes in diabetic rabbit models induced by alloxan
CHEN Pianpian  ZHA Yunfei 

Cite this article as: Chen PP, Zha YF. Quantitative DCE-MRI and QCT were used to evaluate the bone marrow microvascular permeability and trabecular changes in diabetic rabbit models induced by alloxan. Chin J Magn Reson Imaging, 2019, 10(7): 540-545. DOI:10.12015/issn.1674-8034.2019.07.012.


[Abstract] Objective: To quantitatively evaluate the bone marrow microvascular permeability and trabecular changes induced by alloxan in diabetic rabbit model via dynamic contrast enhanced MRI (DCE-MRI) and quantitative CT (QCT).Materials and Methods: Eighteen rabbits were randomly divided into the diabetes group (n=14) and the control group (n=10). Fse-T1WI, Fse-T1WI, DCE-MRI examination and CT imaging were performed at each time point (0, 4, 8, 12 and 16 weeks) after the model had been established successfully. DCE-MRI quantitative perfusion parameters of lumbar bone marrow were obtained by fitting the pharmacokinetic model, including volume transfer constant (Ktrans), efflux rate constant (Kep) and extracellular extravascular volume fraction (Ve). QCT software was applied to measure Lumbar vertebral density (BMD). Lumbar vertebral specimens were collected at week 16 for HE staining to calculate the morphometric parameters of bone trabeculae, including the number of bone trabeculae (Tb.N) and the area of bone trabeculae (Tb.Ar).Results: Osmotic parameters Kep and Ve at different time points indicated statistically significant differences in the control group (P<0.001), which was not significant different in the diabetes group (P>0.05). In the control group, the Kep of lumbar spine showed a descending trend from week 4 to week 12, while Ve displayed an rising trend from week 4 to week 12 and declined from week 16. There was no significant difference in BMD between the control group and the diabetes group at different time points (P>0.05).The results of HE staining exhibited that the number and area of bone trabeculae were decreased in the diabetic group at week 16. The trabecular bone area and trabecular bone count was lower than that of the control group (t=12.472, t=4.961, P<0.001). Pearson correlation analysis results showed that there was no correlation between Tb.N and Ktrans, Kep and Ve (r values: 0.135, 0.093 and -0.118), as well as Tb.Ar and Ktrans, Kep and Ve (r values: 0.233, -0.008 and -0.0.095, P>0.05). No significant correlation was observed between BMD with Ktrans, Kep and Ve (r values:0.497, 0.513 and-0.310, P>0.05).Conclusions: The changes of bone marrow microvascular permeability parameters of the lumbar spine in diabetes mellitus rabbits induced by alloxan in the early stage of disease were not correlated with bone mineral density and trabecular morphometrics parameters, and the differences of bone marrow BMD measured by QCT were later than those of bone trabecular morphometrics.
[Keywords] bone marrow；microvessels；diabetes mellitus；lumbar vertebrae；magnetic resonance imaging；tomography, x-ray；animals, laboratory

CHEN Pianpian Department of Radiology, Renmin Hospital of Wuhan University, Wuhan,430060, China

ZHA Yunfei* Department of Radiology, Renmin Hospital of Wuhan University, Wuhan,430060, China; Hubei Key Laboratory of Medical Information Analysis and Tumor Diagnosis & Treatment, wuhan 430060, China

*Correspondence to: Zha YF, Email: zhayunfei999@126.com

Conflicts of interest   None.

ACKNOWLEDGMENTS  The National Natural Science Foundation of China No. 81871332 Hubei Key Laboratory of Medical Information Analysis and Tumor Diagnosis&Treatment No. PJS140011708
Received  2019-01-28
Accepted  2019-05-22
DOI: 10.12015/issn.1674-8034.2019.07.012
Cite this article as: Chen PP, Zha YF. Quantitative DCE-MRI and QCT were used to evaluate the bone marrow microvascular permeability and trabecular changes in diabetic rabbit models induced by alloxan. Chin J Magn Reson Imaging, 2019, 10(7): 540-545. DOI:10.12015/issn.1674-8034.2019.07.012.

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