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• Clinical Article •
Visualization research on ischemic stroke using aquaporins magnetic resonance molecular imaging
PENG Xiaolan  WENG Ye  HUANG Lidong  YU Bo  CHEN Qiuyan  WEI Dingtai 

Cite this article as: Peng XL, Weng Y, Huang LD, et al. Visualization research on ischemic stroke using aquaporins magnetic resonance molecular imaging. Chin J Magn Reson Imaging, 2019, 10(10): 762-767. DOI:10.12015/issn.1674-8034.2019.10.009.


[Abstract] Objective: To investigate the value of visual imaging in ischemic stroke using multiple b-value diffusion weighted magnetic resonance imaging (MR-DWI), that is aquaporins (AQPs) magnetic resonance molecular imaging.Materials and Methods: A total of 25 Sprague- Dawley rats were underwent transient cerebral ischemic for one hour by middle cerebral artery occlusion (MCAO). Then, the rats were quinquesected randomly into 5 groups: 1, 3, 6, 24, 48 h. Magnetic resonance imaging were performed for all of the 5 groups, containing T2-FLAIR, DWI and AQP-MRI (18b value DWI) scanning series. Relative areas of the lesions were measured for all of the imagings. The areas of AQP-MRI/T2-FLAIR mismatch were compared with that of DWI/T2-FLAIR mismatch. The correlations between MR imagings and TTC staining were analyzed.Results: The location and the range of the lesions, and severity of infarctions were clearly displayed by AQP-MRI. AQP-MRI/T2-FLAIR mismatch showed ischemic penumbra within 24 hours. The result showed that the area of AQP-MRI/T2-FLAIR mismatch was larger than that of DWI/T2-FLAIR mismatch within 24 hours, there was significant difference between them (P<0.01). The aquaporin-apparent diffusivity coefficient (AQP-ADC) values of peripheral to the penumbra in the ipsilateral areas were much higher than that of in the contralateral areas (P<0.05). The microscopic differences between the basal ganglia and ipsilateral distal cortex could be displayed visually in AQP-MR pseudo-color images (P <0.01). There were no significant differences between TTC staining and AQP-MRI, DWI, T2-FLAIR results (P>0.05), and there were significant correlations between them (r=0.903,P=0.004; r=0.935, P=0.02; r=0.872, P=0.054).Conclusions: As a new molecular imaging technology, AQP-MRI dynamic visualized ischemic stroke with multi-layer, multi-angle and more accurately. AQP-MRI had a wide range of potential applications in the diagnosis of ischemic stroke.
[Keywords] stroke；magnetic resonance imaging；ischemic penumbra；aquaporins；visual imaging

PENG Xiaolan Department of Radiology, the Affliated Ningde Municipal Hospital of Fujian Medical University, Ningde 352100, China

WENG Ye Department of Radiology, the Affliated Ningde Municipal Hospital of Fujian Medical University, Ningde 352100, China

HUANG Lidong Department of Radiology, the Affliated Ningde Municipal Hospital of Fujian Medical University, Ningde 352100, China

YU Bo Department of Radiology, the Affliated Ningde Municipal Hospital of Fujian Medical University, Ningde 352100, China

CHEN Qiuyan Department of Radiology, the Affliated Ningde Municipal Hospital of Fujian Medical University, Ningde 352100, China

WEI Dingtai * Department of Radiology, the Affliated Ningde Municipal Hospital of Fujian Medical University, Ningde 352100, China

*Correspondence to: Wei DT, E-mail: wdtai83@163.com

Conflicts of interest   None.

ACKNOWLEDGMENTS  This work was part of National Natural Science Foundation of China No. 81571838 Science and Technology Project of Fujian natural Science Foundation No. 2017J01167, 2018J01227 Fujian Medical University Sailing Fund Project No. 2016QH099 Fujian Province Medical Innovation Project No. 2018-CXB-21
Received  2019-02-18
DOI: 10.12015/issn.1674-8034.2019.10.009
Cite this article as: Peng XL, Weng Y, Huang LD, et al. Visualization research on ischemic stroke using aquaporins magnetic resonance molecular imaging. Chin J Magn Reson Imaging, 2019, 10(10): 762-767. DOI:10.12015/issn.1674-8034.2019.10.009.

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