Share：
Share this content in WeChat
X
[Chinese][PDF]132678
• Clinical Article •
Visualization research on ischemic stroke using aquaporins magnetic resonance molecular imaging
PENG Xiaolan  WENG Ye  HUANG Lidong  YU Bo  CHEN Qiuyan  WEI Dingtai 

Cite this article as: Peng XL, Weng Y, Huang LD, et al. Visualization research on ischemic stroke using aquaporins magnetic resonance molecular imaging. Chin J Magn Reson Imaging, 2019, 10(10): 762-767. DOI:10.12015/issn.1674-8034.2019.10.009.


[Abstract] Objective: To investigate the value of visual imaging in ischemic stroke using multiple b-value diffusion weighted magnetic resonance imaging (MR-DWI), that is aquaporins (AQPs) magnetic resonance molecular imaging.Materials and Methods: A total of 25 Sprague- Dawley rats were underwent transient cerebral ischemic for one hour by middle cerebral artery occlusion (MCAO). Then, the rats were quinquesected randomly into 5 groups: 1, 3, 6, 24, 48 h. Magnetic resonance imaging were performed for all of the 5 groups, containing T2-FLAIR, DWI and AQP-MRI (18b value DWI) scanning series. Relative areas of the lesions were measured for all of the imagings. The areas of AQP-MRI/T2-FLAIR mismatch were compared with that of DWI/T2-FLAIR mismatch. The correlations between MR imagings and TTC staining were analyzed.Results: The location and the range of the lesions, and severity of infarctions were clearly displayed by AQP-MRI. AQP-MRI/T2-FLAIR mismatch showed ischemic penumbra within 24 hours. The result showed that the area of AQP-MRI/T2-FLAIR mismatch was larger than that of DWI/T2-FLAIR mismatch within 24 hours, there was significant difference between them (P<0.01). The aquaporin-apparent diffusivity coefficient (AQP-ADC) values of peripheral to the penumbra in the ipsilateral areas were much higher than that of in the contralateral areas (P<0.05). The microscopic differences between the basal ganglia and ipsilateral distal cortex could be displayed visually in AQP-MR pseudo-color images (P <0.01). There were no significant differences between TTC staining and AQP-MRI, DWI, T2-FLAIR results (P>0.05), and there were significant correlations between them (r=0.903,P=0.004; r=0.935, P=0.02; r=0.872, P=0.054).Conclusions: As a new molecular imaging technology, AQP-MRI dynamic visualized ischemic stroke with multi-layer, multi-angle and more accurately. AQP-MRI had a wide range of potential applications in the diagnosis of ischemic stroke.
[Keywords] stroke；magnetic resonance imaging；ischemic penumbra；aquaporins；visual imaging

PENG Xiaolan Department of Radiology, the Affliated Ningde Municipal Hospital of Fujian Medical University, Ningde 352100, China

WENG Ye Department of Radiology, the Affliated Ningde Municipal Hospital of Fujian Medical University, Ningde 352100, China

HUANG Lidong Department of Radiology, the Affliated Ningde Municipal Hospital of Fujian Medical University, Ningde 352100, China

YU Bo Department of Radiology, the Affliated Ningde Municipal Hospital of Fujian Medical University, Ningde 352100, China

CHEN Qiuyan Department of Radiology, the Affliated Ningde Municipal Hospital of Fujian Medical University, Ningde 352100, China

WEI Dingtai * Department of Radiology, the Affliated Ningde Municipal Hospital of Fujian Medical University, Ningde 352100, China

*Correspondence to: Wei DT, E-mail: wdtai83@163.com

Conflicts of interest   None.

ACKNOWLEDGMENTS  This work was part of National Natural Science Foundation of China No. 81571838 Science and Technology Project of Fujian natural Science Foundation No. 2017J01167, 2018J01227 Fujian Medical University Sailing Fund Project No. 2016QH099 Fujian Province Medical Innovation Project No. 2018-CXB-21
Received  2019-02-18
DOI: 10.12015/issn.1674-8034.2019.10.009
Cite this article as: Peng XL, Weng Y, Huang LD, et al. Visualization research on ischemic stroke using aquaporins magnetic resonance molecular imaging. Chin J Magn Reson Imaging, 2019, 10(10): 762-767. DOI:10.12015/issn.1674-8034.2019.10.009.

[1]
Agre P, Preston GM, Smith BL, et al. Aquaporin CHIP: the archetypal molecular water channel. Am J Physiol, 1993, 265(Pt 2): F463-F476.
[2]
林艳红，孙夕林，程雁，等．水通道蛋白分子成像研究．放射学实践, 2015, 30(6): 622-632.
[3]
Mukherjee A, Wu D, Davis HC, et al. Non-invasive imaging using reporter genes altering cellular water permeability. Nat Commun, 2016, 7: 13891.
[4]
李加慧，李秋菊，于兵，等. DWI-MRI多b值水通道蛋白分子成像机理和方法学研究．中国临床医学影像杂志, 2014, 25(3): 186-189.
[5]
李秋菊，李加慧，赵周社，等. DWI多b值水通道蛋白分子成像在肝纤维化早期诊断的价值．中国临床医学影像杂志, 2014, 25(10): 719-723.
[6]
陈秋雁，吴富淋，彭晓澜，等．水通道蛋白磁共振分子成像与水通道蛋白4表达的相关性研究．中国临床医学影像杂志, 2016, 27(12): 837-841.
[7]
邢培秋，陈秋雁，吴富淋，等．短暂性脑缺血大鼠模型的水通道蛋白磁共振分子影像研究．磁共振成像, 2017, 8(1): 51-56.
[8]
Feigin VL, Forouzanfar MH, Krishnamurthi R, et al. Global and regional burden of stroke during 1990-2010: findings from the global burden of disease study 2010. Lancet, 2014, 383(9913): 245-254.
[9]
Davis S, Donnan GA. Time is penumbra: imaging, selection and outcome. The johann jacob wepfer award 2014. Cerebrovasc Dis, 2014, 38(1): 59-72.
[10]
任国利，袁涛，全冠民．脑缺血半暗带酰胺质子转移成像研究进展．中国医学影像学杂志, 2017, 25(1): 48-51.
[11]
Thirugnanachandran T, Ma H, Singhal S, et al. Refining the ischemic penumbra with topography. Int J Stroke, 2018, 13(3): 277-284.
[12]
Phan TG, Donnan GA, Srikanth V, et al. Heterogeneity in infarct patterns and clinical outcomes following internal carotid artery occlusion. Arch Neurol, 2009, 66(12): 1523-1528.
[13]
Touzani O, Young AR, Derlon JM, et al. Sequential studies of severely hypometabolic tissue volumes after permanent middle cerebral artery occlusion. A positron emission tomographic investigation in anesthetized baboons. Stroke, 1995, 26(11): 2112-2119.
[14]
Donnan GA, Davis SM. Neuroimaging, the ischaemic penumbra, and selection of patients for acute stroke therapy. Lancet Neurol, 2002, 1(7): 417-425.
[15]
索学玲. DWI技术在中枢神经系统中的应用及研究进展．放射学实践, 2018, 33(2): 210-214.
[16]
Cheripelli BK, Huang X, McVerry F, et al. What is the relationship among penumbra volume, collaterals, and time since onset in the first 6 h after acute ischemic stroke?. Int J Stroke, 2016, 11(3): 338-346.
[17]
Kharlamov A, LaVerde GC, Nemoto EM, et al. MAP2 immunostaining in thick sections for early ischemic stroke infarct volume in non-human primate brain. J Neurosci Methods, 2009, 182(2): 205-210.
[18]
Grohn OH, Lukkarinen JA, Oja JM, et al. Noninvasive detection of cerebral hypoperfusion and reversible ischemia from reductions in the magnetic resonance imaging relaxation time, T2. J Cereb Blood Flow Metab, 1998, 18(8): 911-920.
[19]
Mack WJ, Komotar RJ, Mocco J, et al. Serial magnetic resonance imaging in experimental primate stroke: validation of MRI for pre-clinical cerebroprotective trials. Neurol Res, 2003, 25(8): 846-852.
[20]
Saver JL, Fonarow GC, Smith EE, et al. Time to treatment with intravenous tissue plasminogen activator and outcome from acute ischemic stroke. JAMA, 2013, 309(23): 2480-2488.

PREV Value of PDW-SPAIR in visual assessment of active lesions in patients with rheumatoid arthritis
NEXT Control study on multimode function magnetic resonance imaging of wistar rat epilepsy model and its relationship with histopathology
  


LINK


Tel & Fax: +8610-67113815    E-mail: editor@cjmri.cn