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• Original Article •
USPIO-DSC MRI study on acupuncture improving cerebral blood flow of pMCAO rats by regulating levels of inflammatory cytokines
YANG Shuohui  WU Yu  ZHAN Songhua  YANG Chenyao  KONG Yingnan  ZHANG Cheng  LU Fang 

Cite this article as: Yang SH, Wu Y, Zhan SH, et al. USPIO-DSC MRI study on acupuncture improving cerebral blood flow of pMCAO rats by regulating levels of inflammatory cytokines. Chin J Magn Reson Imaging, 2020, 11(7): 557-562. DOI:10.12015/issn.1674-8034.2020.07.016.


[Abstract] Objective: To evaluate the effectiveness of acupuncture on improving CBF and CBV within infarcted areas during acute-stage by regulating serum levels of inflammatory cytokines in permanent middle cerebral artery occlusion (pMCAO) model rats with ultrasmall superparamagnetic particles of iron oxides (USPIO) dynamic susceptibility contrast (DSC) MRI.Materials and Methods: Twenty-four SD rats models were established, including sham-operation group (group A, n=6), pMCAO group without acupuncture (group B, n=10), pMCAO group with electroacupuncture (group C, n=8). Each group was further divided into 2 subgroups according to 24 h and 48 h after modeling. Rats in the group C were treated with electroacupuncture on Baihui, Shuigou and right Zusanli acupoints. Every rat in each group was scanned with USPIO-DSC on a 1.5 T MR scanner. The values of the relative cerebral blood volume (rCBV) and the relative cerebral blood flow (rCBF) on regions of infarct and contralateral in mirror areas were measured. The ratios (infarct/contralateral) of rrCBV and rrCBF were calculated. These ratios on 24 h and 48 h were compared to evaluate whether there were any statistical differences among Group A, B and C. Spearman correlation coefficients were calculated between the ratios rrCBV and rrCBF of Group B plus C and values of IL-1β, IL-6, IL-10 and TNF-α, respectively.Results: The values of rrCBV and rrCBF were significantly higher in group A than those in group B and C on 24 h and 48 h (P<0.05). The values of rrCBV and rrCBF were significantly higher in group C than those in group B on 24 h and 48 h (P <0.05). On 24 h, the values of rrCBV and rrCBF of group B plus C were significantly negatively correlated with the values of IL-1β and IL-6, and positively correlated with the values of IL-10 (P<0.05). The values of rrCBV of group B plus C were significantly positively correlated with the values of TNF-α on 24 h (P<0.05). On 48 h, the values of rrCBV and rrCBF of group B plus C were significantly negatively correlated with the values of IL-1β, and positively correlated with the values of IL-10 (P<0.05).Conclusions: The blood supply of infarct areas during acute stage in pMCAO rats is improved on acupuncture by down-regulating the serum levels of IL-1β and IL-6 to reduce the inflammatory response and up-regulating the serum levels of IL-10 and TNF-α to increase the rrCBV and rrCBF in the infarct areas.
[Keywords] ultrasmall superparamagnetic particles of iron oxides；magnetic resonance imaging；inflammatory cytokines；acupuncture；infarct

YANG Shuohui Department of Radiology, Shuguang Hospital Affiliated to Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China

WU Yu Department of Radiology, Shuguang Hospital Affiliated to Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China

ZHAN Songhua Department of Radiology, Shuguang Hospital Affiliated to Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China

YANG Chenyao Department of Radiology, Shuguang Hospital Affiliated to Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China

KONG Yingnan Department of Radiology, Shuguang Hospital Affiliated to Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China

ZHANG Cheng Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China

LU Fang* Department of Radiology, Shuguang Hospital Affiliated to Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China

*Correspondence to: Lu F, E-mail: lufang800@aliyun.com

Conflicts of interest   None.

ACKNOWLEDGMENTS  This paper is supported by the Youth Program of National Natural Science Foundation of China No. 81403204 and supported by the General Program of National Natural Science Foundation of China No. 81573782
Received  2020-03-04
Accepted  2020-05-21
DOI: 10.12015/issn.1674-8034.2020.07.016
Cite this article as: Yang SH, Wu Y, Zhan SH, et al. USPIO-DSC MRI study on acupuncture improving cerebral blood flow of pMCAO rats by regulating levels of inflammatory cytokines. Chin J Magn Reson Imaging, 2020, 11(7): 557-562. DOI:10.12015/issn.1674-8034.2020.07.016.

[1]
Benjamin EJ, Virani SS, Callaway CW, et al. Heart disease and stroke statistics-2018 update: a report from the American Heart Association. Circulation, 2018, 137: e67-e492.
[2]
Vidale S, Consoli A, Arnaboldi M, et al. Postischemic inflammation in acute stroke. J Clin Neurol, 2017, 13(1): 1-9.
[3]
陆方，詹松华，成建明，等．针刺减轻永久性大鼠局灶性脑缺血亚急性期炎性反应的USPIO增强MRI研究初探．医学影像学杂志, 2017, 27(10): 1996-2000.
[4]
钱宇斐，樊小农，李雅洁，等．针刺不同穴位对脑缺血模型大鼠脑血流量的影响．中国针灸, 2009, 29(3): 213-216.
[5]
徐畅,邰东梅，马贤德，等．眼针和体针对24 h脑缺血再灌注损伤模型大鼠脑血流和脑源性神经营养因子表达的影响．中华中医药学刊, 2019, 37(9): 2178-2181.
[6]
杨丽红，杜元灏，李晶，等．电针对脑梗死大鼠脑血管内皮细胞Apelin-APJ系统的影响．针刺研究, 2017, 42(1): 9-13.
[7]
Du Y, Shi L, Li J, et al. Angiogenesis and improved cerebral blood flow in the ischemic boundary area were detected after electroacupuncture treatment to rats with ischemic stroke. Neurol Res, 2011, 33(1): 101-107.
[8]
Kim SG, Harel N, Jin T, et al. Cerebral blood volume MRI with intravascular superparamagentic iron oxide nanoparticles. NMR Biomed, 2013, 26(8): 949-962.
[9]
Longa EZ, Weinstein PR, Carlson S, et al. Reversible middle cerebral artery occlusion without craniectomy in rats. Stroke, 1989, 20(1): 84-91.
[10]
李忠仁．实验针灸学．北京：中国中医药出版社, 2007: 359-362.
[11]
骆明军，程玲，刘胜洪，等．电针对缺血再灌注大鼠脑内小胶质细胞活化的影响．中国针灸, 2004, 24(5): 351-353.
[12]
Varallyay CG, Muldoon LL, Gahramanov S, et al. Dynamic MRI using iron oxide nanoparticles to assess early vascular effects of antiangiogenic versus corticosteroid treatment in a glioma model. J Cereb Blood Flow Metab, 2009, 29(4): 853-860.
[13]
Qin C, Zhou LQ, Ma XT, et al. Dual functions of microglia in ischemic stroke. Neurosci Bull, 2019, 35(5): 921-933.
[14]
Mizuma A, Yenari MA. Anti-inflammatory targets for the treatment of reperfusion injury in stroke. Front Neurol, 2017, 8: 467.
[15]
Protti GG, Gagliardi RJ, Forte WC, et al. Interleukin-10 may protect against progressing injury during the acute phase of ischemic stroke. Arq Neuropsiquiatr, 2013, 71: 846-851.
[16]
Geng Y, Chen Y, Sun W, et al. Electroacupuncture ameliorates cerebral I/R-induced inflammation through DOR-BDNF/TrkB pathway. Evid Based Complement Alternat Med, 2020, 2020: 3495836.
[17]
Lan L, Tao J, Chen A, et al. Electroacupuncture exerts anti-inflammatory effects in cerebral ischemia-reperfusion injured rats via suppression of the TLR4/NF-κB pathway. Int J Mol Med, 2013, 31(1): 75-80.
[18]
Jiang J, Luo Y, Qin W, et al. Electroacupuncture suppresses the NF-κB signaling pathway by upregulating cylindromatosis to alleviate inflammatory injury in cerebral ischemia/reperfusion rats. Front Mol Neurosci, 2017, 10: 363.
[19]
王金海，张婷卓，李兴兰，等．头穴针刺对局灶性脑缺血大鼠海马旁回炎性反应相关细胞因子表达及脑梗死体积的影响．针刺研究, 2019, 44(6): 405-411.
[20]
Liu J, Wang Y, Akamatsu Y, et al. Vascular remodeling after ischemic stroke: mechanisms and therapeutic potentials. Prog Neurobiol, 2014, 115: 138-56.
[21]
Shi L, Cao HM, Li Y, et al. Electroacupuncture improves neurovascular unit reconstruction by promoting collateral circulation and angiogenesis. Neural Regen Res, 2017, 12(2): 2000-2006.
[22]
Han B, Lu Y, Zhao H, et al. Electroacupuncture modulated the inflammatory reaction in MCAO rats via inhibiting the TLR4/NF-κB signaling pathway in microglia. Int J Clin Exp Pathol, 2015, 8(9): 11199-205.
[23]
吕艳，杜元灏，徐彦龙，等．电针"水沟"穴对大脑中动脉梗死大鼠脑血管平滑肌蛋白激酶C的影响．针刺研究, 2015, 40(3): 219-223.
[24]
Sriram K, O’Callaghan JP. Divergent roles for tumor necrosis factor-alpha in the brain. J Neuroimmune Pharmacol, 2007, 2(2): 140-53.
[25]
赵培，朱金墙，梁玉彬，等．肿瘤坏死因子-α在脑缺血再灌注炎症损伤中的作用．中国老年学杂志, 2017, 37(14): 3628-3630.

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