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• Original Article •
In vitro study of SR-A targeted multimodal nanoparticles for the detection of atherosclerotic vulnerable plaques
YE Man  ZHONG Yixin  WANG Xingyue  GUO Dajing  ZHOU Jun 

Cite this article as: Ye M, Zhong YX, Wang XY, et al. In vitro study of SR-A targeted multimodal nanoparticles for the detection of atherosclerotic vulnerable plaques. Chin J Magn Reson Imaging, 2020, 11(10): 885-889. DOI:10.12015/issn.1674-8034.2020.10.010.


[Abstract] Objective: To prepare a dualmodal molecular probe targeting scavenger receptor A (SR-A) loaded with dextran sulfate (DS), and to investigate its magnetic resonance (MR)/enhanced photoacoustic (PA) imaging and targeting ability in vitro.Materials and Methods: Nanoparticles [poly (lactic-co-glycolic acid), PLGA]-Fe3O4-DS were synthesized using double emulsification method and electrostatic adsorption method. The physicochemical properties and the MR/PA imaging capability were observed in vitro. The targeting efficiency of molecular probes for activated macrophages were evaluated.Results: The prepared PLGA-Fe3O4-DS nanoparticles have a regular shape, uniform size, and good dispersibility. The average particle diameter is 263.93±21.38 nm, the Zeta potential is -20.63±2.61 mV, and the DS connection rate is 90.06%. Nanoparticles have a good negative enhancement effect on magnetic resonance imaging. With the increase of its concentration, the photoacoustic signal gradually increases. Compared with the non-targeted group (PLGA-Fe3O4), the PLGA-Fe3O4-DS nanoparticles accumulated in large numbers around the nucleus after co-incubation of nanoparticles with activated macrophages.Conclusions: The PLGA-Fe3O4-DS nanoparticles were successfully prepared, which have good MR/PA imaging capabilities and high affinity for SR-A, providing the potential of analyzing the pathophysiological mechanism of receptors in the development of atherosclerotic plaques at the molecular level.
[Keywords] dextran sulfate；scavenger receptor A；magnetic resonance imaging；photoacoustic imaging；atherosclerotic

YE Man Department of Radiology, Renmin Hospital of Wuhan University, Wuhan 430060, China; Department of Radiology, the Second Affiliated Hospital of Chongqing Medical University, Chongqing 400010, China

ZHONG Yixin Department of Radiology, the Second Affiliated Hospital of Chongqing Medical University, Chongqing 400010, China

WANG Xingyue Chongqing Key Laboratory of Ultrasound Molecular Imaging, Chongqing 400010, China

GUO Dajing Department of Radiology, the Second Affiliated Hospital of Chongqing Medical University, Chongqing 400010, China

ZHOU Jun* Department of Radiology, the Second Affiliated Hospital of Chongqing Medical University, Chongqing 400010, China

*Correspondence to: Zhou J, E-mail: 659791972@qq.com

Conflicts of interest   None.

ACKNOWLEDGMENTS  This work was part of National Natural Science Foundation of China No. 81701650 Postdoctoral Talent Support Program No. 2019M663891XB
Received  2020-03-12
Accepted  2020-05-08
DOI: 10.12015/issn.1674-8034.2020.10.010
Cite this article as: Ye M, Zhong YX, Wang XY, et al. In vitro study of SR-A targeted multimodal nanoparticles for the detection of atherosclerotic vulnerable plaques. Chin J Magn Reson Imaging, 2020, 11(10): 885-889. DOI:10.12015/issn.1674-8034.2020.10.010.

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