Limb remote ischemic preconditioning prolongs the existence window of graphic penumbra in cerebral ischemia rat model and its related mechanism: through inhibiting the expression of high mobility group protein 1

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• Original Article •

WU Fulin YU Bo WENG Ye PENG Xiaolan WEI Dingtai CHEN Qiuyan

Cite this article as: Wu FL, Yu B, Weng Y, et al. Limb remote ischemic preconditioning prolongs the existence window of graphic penumbra in cerebral ischemia rat model and its related mechanism: through inhibiting the expression of high mobility group protein 1. Chin J Magn Reson Imaging, 2020, 11(11): 1023-1028. DOI:10.12015/issn.1674-8034.2020.11.013.

Abstract

[Abstract] Objective: To investigate the effect of limb remote ischemic preconditioning (LRP) on the ischemic penumbra in a cerebral ischemia rat model, and to explore whether the neuroprotective effect of LRP might be related to the expression of high mobility group protein 1 (HMGB1).Materials and Methods: Seventy-two adult male Sprague-Dawley rats were randomized into 3 groups: cerebral ischemia group, LRP group and sham controls. All rats underwent MRI detection to acquire T2-FLAIR and diffusion-weighted imaging (DWI) data at different time points (1 h, 3 h, 6 h and 24 h). TTC staining and behavioral score were subsequently executed. Western Blot was used to detect the expression of HMGB1, and Real-time PCR was used to detect the transcription level of HMGB1.Results: LRP improved the behavioral outcomes (2.60±0.65 vs. 1.52±0.65, P<0.001) and reduced the infarct size (68.04±13.24 vs. 29.02±23.62, P<0.001). MRI results indicated that LRP could delay the lesion (P<0.001) and prolong the existence window of the penumbra (P<0.001).Western blot and PCR analysis showed that LRP down-regulated protein expression and transcription level of HMGB1 (P<0.05).Conclusions: LRP can prolong the existence of penumbra in ischemic stroke, which may prolong the treatment time window. The neuroprotective effect of LRP is related to the expression of HMGB1.

[Keywords] limb remote ischemic preconditioning；graphic penumbra；high mobility group protein 1；magnetic resonance imaging；diffusion weighted imaging

Contributor Information

WU Fulin Department of Radiology, Fujian Medical University Ningde Hospital, Ningde 352100, China

YU Bo Department of Radiology, Fujian Medical University Ningde Hospital, Ningde 352100, China; Functional and Molecular Imaging Laboratory for Cerebral Vascular Diseases, Fujian Medical University Ningde Hospital, Ningde 352100, China

WENG Ye Department of Radiology, Fujian Medical University Ningde Hospital, Ningde 352100, China

PENG Xiaolan Department of Radiology, Fujian Medical University Ningde Hospital, Ningde 352100, China

WEI Dingtai Department of Radiology, Fujian Medical University Ningde Hospital, Ningde 352100, China; Functional and Molecular Imaging Laboratory for Cerebral Vascular Diseases, Fujian Medical University Ningde Hospital, Ningde 352100, China

CHEN Qiuyan^* Department of Radiology, Fujian Medical University Ningde Hospital, Ningde 352100, China; Functional and Molecular Imaging Laboratory for Cerebral Vascular Diseases, Fujian Medical University Ningde Hospital, Ningde 352100, China

*Correspondence to: Chen QY, E-mail: lc7505723@163.com

Conflicts of interest None.

Publication Information

ACKNOWLEDGMENTS This study was supported by National Natural Science Foundation of China No. 81571838 Fujian Natural Science Foundation No. 2017J01167, 2018J01227 Fujian Medical Innovation Project No. 2018-CXB-21 Fujian Medical University Startup Fund for Scientific Research No. 2016QH099

Received 2019-12-17

Accepted 2020-09-18

DOI: 10.12015/issn.1674-8034.2020.11.013

Text

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