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• Original Article •
USPIO enhanced SWI MRI to evaluate the effects of cinobufacini on a nude orthotopic hepatocellular carcinoma tumor model
LU Fang  GUO Ran  LIN Jiang  YANG Shuohui  FU Caixia  LIU Mengxiao  YU Jianli 

Cite this article as: Lu F, Guo R, Lin J, et al. USPIO enhanced SWI MRI to evaluate the effects of cinobufacini on a nude orthotopic hepatocellular carcinoma tumor model[J]. Chin J Magn Reson Imaging, 2021, 12(12): 44-48. DOI:10.12015/issn.1674-8034.2021.12.009.


[Abstract] Objective To assess the validity of antiangiogenic effects of cinobufacini on nude orthotopic hepatocellular carcinoma (HCC) xenografts with ultrasmall superparamagnetic iron oxide enhanced susceptibility-weighted imaging (USPIO enhanced SWI). Materials and Methods: A total of 16 nude HCC models were made. They were divided into cinobufacini treated and saline control groups randomly with eight in each at twenty-one days after tumor inoculation. USPIO enhanced SWI were performed on a 3.0 T Siemens MR scanner after another twenty-one days with intervention. Intratumoral susceptibility signal intensity (ITSS) of tumor was scored. They were compared between two groups. The correlations between ITSS, micro-vessel density (MVD) and tumor volume were analyzed.Results There were 5 mice survived in the treated group and 7 in the saline control group. ITSS was significantly lower in the treated than in the saline control group (P＜0.05). In 12 mice, significantly good positive correlation was found between ITSS and MVD (r=0.647, P=0.022), and ITSS correlated moderately with tumor volume (r=0.645, P=0.023).Conclusions This USPIO enhanced SWI MRI study showed that cinobufosin can inhibit the formation of large blood vessels in tumors of nude HCC mice.
[Keywords] ultrasmall superparamagnetic iron oxide；susceptibility-weighted imaging；cinobufacini；hepatocellular carcinoma；magnetic resonance imaging

LU Fang1   GUO Ran2, 3   LIN Jiang2, 3   YANG Shuohui4*   FU Caixia5   LIU Mengxiao6   YU Jianli4  

1 Department of Radiology, Shuguang Hospital Affiliated to Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China

2 Department of Radiology, Zhongshan Hospital Affiliated to Fudan University, Shanghai 200031, China

3 Shanghai Institute of Medical Imaging, Shanghai 200031, China

4 Department of Radiology, Shanghai Municipal Hospital of Traditional Chinese Medicine, Shanghai University of Traditional Chinese Medicine, Shanghai 200071, China

5 Siemens Shenzhen Magnetic Resonance Ltd., Shenzhen 518057, China

6 MR scientific Marketing, Siemens Healthcare, Shanghai 201318, China

Yang SH, E-mail: caddie_yang1980@aliyun.com

Conflicts of interest   None.

ACKNOWLEDGMENTS Shanghai "Rising Stars of Medical Talent" Youth Development Program Youth Medical Talents—Medical Imaging Practitioner Program [No. SHWRS (2020)-087].
Received  2021-06-30
Accepted  2021-09-24
DOI: 10.12015/issn.1674-8034.2021.12.009
Cite this article as: Lu F, Guo R, Lin J, et al. USPIO enhanced SWI MRI to evaluate the effects of cinobufacini on a nude orthotopic hepatocellular carcinoma tumor model[J]. Chin J Magn Reson Imaging, 2021, 12(12): 44-48. DOI:10.12015/issn.1674-8034.2021.12.009.

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