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• Original Article •
Experimental study on the evaluation of acute mesenteric artery ischemia in the small intestine wall injury of rabbits by quantitative magnetic resonance T2-mapping technique
CHEN Mengsha  ZU Hanyu  ZHONG Xiaofei  LI Minmin  CAO Zhang  HAN Huiting  CAO He  JIANG Xingyue 

Cite this article as: Chen MS, Zu HY, Zhong XF, et al. Experimental study on the evaluation of acute mesenteric artery ischemia in the small intestine wall injury of rabbits by quantitative magnetic resonance T2-mapping technique[J]. Chin J Magn Reson Imaging, 2022, 13(9): 69-74, 80. DOI:10.12015/issn.1674-8034.2022.09.013.


[Abstract] Objective To explore the diagnostic value of T2-mapping in quantitatively monitoring and assessing dynamic small intestine wall injury after acute mesenteric artery ischemia (AMI) in a rabbit model by MRI.Materials and Methods Thirty-six New Zealand white rabbits were randomly divided into two groups. The experimental group performed surgical ligation of the arc-shaped vascular network of the 2nd-5th mesenteric artery and the blood supply vessels at both ends of the corresponding intestinal tract, followed by MR T2-mapping imaging at 6 time points (1 h, 2 h, 3 h, 4 h, 5 h, 6 h), 3 rabbits at each time point. After scanning, 3 rabbits were euthanized, and the intestinal pathological specimens supplied by the third branch of the mesenteric artery arc vascular network were taken out to evaluate the severity of ischemic damage to the small intestinal wall and the changes in pathological characteristics. The control group underwent sham operation without ligation. The differences between the quantitative values of the experimental group and the control group at each time point were performed by independent samples t test, and the intra-group comparison of T2 values at each time point between the two groups was performed by one-way analysis of variance. A Gaussian fitting model was used to fit the T2 value to the ischemic time point to show the evolution of the ischemic intestinal wall in the small intestine, which was verified by histopathology. The fitting equation between T2 value and ischemic time point is f(x)=145×exp{-[(x-3.475)/4.297]2}, and the fitting coefficient R2=0.79.Results The T2 values of the experimental group at 6 time points were higher than those of the control group, and the difference was statistically significant (P＜0.05). There were statistical differences between T23h and T21h, T22h, T25h and T26h in the experimental group (P＜0.05). With the progression of ischemia time, the T2 value first increased and then decreased. The pathological manifestations in the first 3 hours were mainly edema and infiltration of inflammatory cells. The T23h value reached a peak value of (151.50±2.90) ms at nearly 3 hours of ischemia on the image; at 4 h of ischemia, the intestinal wall injury reached the muscle layer, and the bleeding area greatly increased, the T24h value decreased to (142.50±4.30) ms, and the T2 value continued to decrease thereafter.Conclusions MRI T2-mapping technology helps to quantitatively evaluate the small intestinal wall damage in AMI, has certain advantages for early detection of the disease, and has a good clinical application prospect.
[Keywords] T2-mapping；quantitative techniques；magnetic resonance imaging；acute mesenteric ischemia；rabbit animal model；small bowel wall damage

CHEN Mengsha1   ZU Hanyu1   ZHONG Xiaofei1   LI Minmin1   CAO Zhang2   HAN Huiting1   CAO He1   JIANG Xingyue1*  

1 Department of Radiology, Affiliated Hospital of Binzhou Medical University, Binzhou 256603

2 Department of Pathology, Affiliated Hospital of Binzhou Medical University, Binzhou 256603

*Jiang XY, E-mail: xyjiang188@sina.com

Conflicts of interest   None.

ACKNOWLEDGMENTS Natural Science Foundation of Shandong Province (No. ZR2018LH015)
Received  2022-06-06
Accepted  2022-09-13
DOI: 10.12015/issn.1674-8034.2022.09.013
Cite this article as: Chen MS, Zu HY, Zhong XF, et al. Experimental study on the evaluation of acute mesenteric artery ischemia in the small intestine wall injury of rabbits by quantitative magnetic resonance T2-mapping technique[J]. Chin J Magn Reson Imaging, 2022, 13(9): 69-74, 80. DOI:10.12015/issn.1674-8034.2022.09.013.

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