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• Original Articles •
Quantitative analysis of early changes in the myelin structure of rats with ischemic stroke using MRI signal intensity ratio and diffusion tensor imaging
WENG Na  SHAO Cuijie  LI Guodong  BIN Li  HUANG Danqi  WANG Xu 

Cite this article as: Weng N, Shao CJ, Li GD, et al. Quantitative analysis of early changes in the myelin structure of rats with ischemic stroke using MRI signal intensity ratio and diffusion tensor imaging[J]. Chin J Magn Reson Imaging, 2022, 13(10): 150-156. DOI:10.12015/issn.1674-8034.2022.10.023.


[Abstract] Objective To explore the early diagnostic value of signal intensity ratio (SIR) and diffusion tensor imaging (DTI) in myelin structural changes in the white matter of ischemic stroke rats.Materials and Methods Eighteen Sprague Dawley rats were divided into three groups, Groups A, B, and C, with six rats in each group. Middle cerebral artery occlusion (MCAO) sham operation without ligation was performed in group A, the 24 hours after MCAO operation in group B, and the 72 hours after MCAO operation in group C. After obtaining T1WI, T2WI, and DTI scans under the same conditions, post-processing to get the SIR, fractional anisotropy (FA), apparent diffusion coefficient (ADC), radial diffusivity (D⊥) imaging. The corpus callosum and external capsule regions were outlined to calculate the SIR value, FA value, ADC value, and D⊥ value, and a one-way analysis of variance was performed among the three groups. At the end of scanning, the samples were obtained for luxol fast blue (LFB) -tar purple pathological staining, and the mean grayscale value (MGSV) was measured. One-way analysis of variance between the three groups was performed. Pairwise correlation analysis was performed between the statistically significant imaging data and the MGSV.Results Compared with group A, group B had decreased SIR (P=0.044), fractional anisotropy (FA; P=0.001), apparent diffusion coefficient (ADC; P=0.001) and radial diffusion coefficient (D⊥; P=0.001) values in the external capsule region. Group C had decreased SIR (P＜0.001) and FA (P=0.002) in the external capsule region. SIR (P=0.031) and FA (P=0.015) in the corpus callosum were decreased. LFB-tar purple poorly stained the areas of the myelin sheath that were damaged due to stroke, and the mean grayscale value (MGSV) decreased (P＜0.05). In the external capsule region, MGSV was negatively correlated with SIR (P＜0.0001), FA (P≤0.0001), ADC (P=0.0127), and D⊥ (P=0.0180). In the corpus callosum region, MGSV was negatively correlated with SIR (P=0.0018).Conclusions A combination of SIR and DTI can be used as a noninvasive imaging method to evaluate the different stages of myelin injury in ischemic stroke. This has been proven effective in rat models and provides a helpful imaging basis for clinical diagnosis and treatment.
[Keywords] ischemia stroke；demyelination；MCAO model；signal intensity ratio；magnetic resonance imaging；diffusion tensor imaging

WENG Na1   SHAO Cuijie2   LI Guodong1   BIN Li1   HUANG Danqi1   WANG Xu1*  

1 Department of Nuclear Medicine, Binzhou Medical University Hospital, Binzhou 256600, China

2 Medical Research Center, Binzhou Medical University Hospital, Binzhou 256600, China

Wang X, E-mail:wangxu1978@163.com

Conflicts of interest   None.

ACKNOWLEDGMENTS National Natural Science Foundation of China (No. 81771828).
Received  2022-07-05
Accepted  2022-09-30
DOI: 10.12015/issn.1674-8034.2022.10.023
Cite this article as: Weng N, Shao CJ, Li GD, et al. Quantitative analysis of early changes in the myelin structure of rats with ischemic stroke using MRI signal intensity ratio and diffusion tensor imaging[J]. Chin J Magn Reson Imaging, 2022, 13(10): 150-156. DOI:10.12015/issn.1674-8034.2022.10.023.

[1]
Feske SK. Ischemic stroke[J]. Am J Med, 2021, 134(12): 1457-1464. DOI: 10.1016/j.amjmed.2021.07.027.
[2]
Members WG, Mozaffarian D, Benjamin EJ, et al. Heart disease and stroke statistics-2016 update: a report from the American heart association[J/OL]. Circulation, 2016 [2022-07-05]. https://doi.org/10.1161/cir.0000000000000350. DOI: 10.1161/cir.0000000000000350.
[3]
Baron JC. Protecting the ischaemic penumbra as an adjunct to thrombectomy for acute stroke[J]. Nat Rev Neurol, 2018, 14(6): 325-337. DOI: 10.1038/s41582-018-0002-2.
[4]
Wan T, Zhu WS, Zhao Y, et al. Astrocytic phagocytosis contributes to demyelination after focal cortical ischemia in mice[J/OL]. Nat Commun, 2022 [2022-07-05]. https://doi.org/10.1038/s41467-022-28777-9. DOI: 10.1038/s41467-022-28777-9.
[5]
Egorova N, Dhollander T, Khlif MS, et al. Pervasive white matter fiber degeneration in ischemic stroke[J]. Stroke, 2020, 51(5): 1507-1513. DOI: 10.1161/STROKEAHA.119.028143.
[6]
Fields RD. A new mechanism of nervous system plasticity: activity-dependent myelination[J]. Nat Rev Neurosci, 2015, 16(12): 756-767. DOI: 10.1038/nrn4023.
[7]
Gladstone DJ, Black SE, Hakim AM, et al. Toward wisdom from failure: lessons from neuroprotective stroke trials and new therapeutic directions[J]. Stroke, 2002, 33(8): 2123-2136. DOI: 10.1161/01.str.0000025518.34157.51.
[8]
Li YF, Liu Z, Song YY, et al. M2 microglia-derived extracellular vesicles promote white matter repair and functional recovery via miR-23a-5p after cerebral ischemia in mice[J]. Theranostics, 2022, 12(7): 3553-3573. DOI: 10.7150/thno.68895.
[9]
Glasser MF, Van Essen DC. Mapping human cortical areas in vivo based on myelin content as revealed by T1- and T2-weighted MRI[J]. J Neurosci, 2011, 31(32): 11597-11616. DOI: 10.1523/jneurosci.2180-11.2011.
[10]
Glasser MF, Goyal MS, Preuss TM, et al. Trends and properties of human cerebral cortex: correlations with cortical myelin content[J/OL]. NeuroImage, 2014 [2022-07-05]. https://doi.org/10.1016/j.neuroimage.2013.03.060. DOI: 10.1016/j.neuroimage.2013.03.060.
[11]
Nakamura K, Chen JT, Ontaneda D, et al. T1-/ T2-weighted ratio differs in demyelinated cortex in multiple sclerosis[J]. Ann Neurol, 2017, 82(4): 635-639. DOI: 10.1002/ana.25019.
[12]
Petracca M, El Mendili MM, Moro M, et al. Laminar analysis of the cortical T1/T2-weighted ratio at 7T[J/OL]. Neurol Neuroimmunol Neuroinflamm, 2020 [2022-07-05]. https://doi.org/10.1212/nxi.0000000000000900 . DOI: 10.1212/NXI.0000000000000900.
[13]
Filip P, Canna A, Moheet A, et al. Structural alterations in deep brain structures in type 1 diabetes[J]. Diabetes, 2020, 69(11): 2458-2466. DOI: 10.2337/db19-1100.
[14]
Yang L, Li MZ, Zhan Y, et al. The impact of ischemic stroke on gray and white matter injury correlated with motor and cognitive impairments in permanent MCAO rats: a multimodal MRI-based study[J/OL]. Front Neurol, 2022 [2022-07-05]. https://doi.org/10.3389/fneur.2022.834329. DOI: 10.3389/fneur.2022.834329.
[15]
Jung WB, Han YH, Chung JJ, et al. Spatiotemporal microstructural white matter changes in diffusion tensor imaging after transient focal ischemic stroke in rats[J/OL]. NMR Biomed, 2017 [2022-07-05]. https://doi.org/10.1002/nbm.3704. DOI: 10.1002/nbm.3704.
[16]
Moura LM, Luccas R, de Paiva JPQ, et al. Diffusion tensor imaging biomarkers to predict motor outcomes in stroke: a narrative review[J/OL]. Front Neurol, 2019 [2022-07-05]. https://doi.org/10.3389/fneur.2019.00445. DOI: 10.3389/fneur.2019.00445.
[17]
Beaulieu C. The basis of anisotropic water diffusion in the nervous system - a technical review[J]. NMR Biomed, 2002, 15(7/8): 435-455. DOI: 10.1002/nbm.782.
[18]
McBride DW, Zhang JH. Precision stroke animal models: the permanent MCAO model should be the primary model, not transient MCAO[J/OL]. Transl Stroke Res, 2017 [2022-07-05]. https://doi.org/10.1007/s12975-017-0554-2. DOI: 10.1007/s12975-017-0554-2.
[19]
Ding S, Du SL, Zhu QY, et al. Quantitative analysis of demyelination mouse model using signal intensity ratio and diffusion tensor imaging at 7.0 Tesla MRI[J]. J Third Mil Med Univ, 2021, 43(13): 1227-1234. DOI: 10.16016/j.1000-5404.202101113.
[20]
Khodanovich MY, Kisel AA, Akulov AE, et al. Quantitative assessment of demyelination in ischemic stroke in vivo using macromolecular proton fraction mapping[J]. J Cereb Blood Flow Metab, 2018, 38(5): 919-931. DOI: 10.1177/0271678x18755203.
[21]
Khodanovich MY, Gubskiy IL, Kudabaeva MS, et al. Long-term monitoring of chronic demyelination and remyelination in a rat ischemic stroke model using macromolecular proton fraction mapping[J]. J Cereb Blood Flow Metab, 2021, 41(11): 2856-2869. DOI: 10.1177/0271678X211020860.
[22]
Kisel AA, Naumova AV, Yarnykh VL. Macromolecular proton fraction as a myelin biomarker: principles, validation, and applications[J/OL]. Front Neurosci, 2022 [2022-07-05]. https://doi.org/10.3389/fnins.2022.819912. DOI: 10.3389/fnins.2022.819912.
[23]
Park M, Cho Y, Kim DH, et al. Myelin water imaging of nerve recovery in rehabilitating stroke patients[J/OL]. J Magn Reson Imaging, 2022 [2022-07-05]. https://doi.org/10.1002/jmri.28185. DOI: 10.1002/jmri.28185.
[24]
Uddin MN, Figley TD, Marrie RA, et al. Can T 1 w/T 2 w ratio be used as a myelin-specific measure in subcortical structures? Comparisons between FSE-basedT1w/T2ratiosw, GRASE-basedT1w/T2w ratios and multi-echo GRASE-based myelin water fractions[J/OL]. NMR Biomed, 2018 [2022-07-05]. https://doi.org/10.1002/nbm.3868. DOI: 10.1002/nbm.3868.
[25]
Sibon I, Tourdias T, Felix S, et al. Magnetisation transfer parameters and stroke outcome[J]. J Clin Neurosci, 2015, 22(6): 1012-1017. DOI: 10.1016/j.jocn.2014.11.035.
[26]
Sozmen EG, Kolekar A, Havton LA, et al. A white matter stroke model in the mouse: axonal damage, progenitor responses and MRI correlates[J]. J Neurosci Methods, 2009, 180(2): 261-272. DOI: 10.1016/j.jneumeth.2009.03.017.
[27]
Ma Z, Zhao X, Wang X, et al. Evaluation of crossed cerebellar diaschisis after cerebral infarction in MCAO rats based on DKI[J/OL]. Eur J Clin Invest, 2022, 52(4) [2022-07-05]. https://onlinelibrary.wiley.com/doi/10.1111/eci.13716. DOI: 10.1111/eci.13716.
[28]
Ding S, Guo Y, Chen XY, et al. Demyelination and remyelination detected in an alternative cuprizone mouse model of multiple sclerosis with 7.0 T multiparameter magnetic resonance imaging[J/OL]. Sci Rep, 2021 [2022-07-05]. https://doi.org/10.1038/s41598-021-90597-6. DOI: 10.1038/s41598-021-90597-6.

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