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• Original Article •
Multimodal MRI manifestations and pathological basis of peritumoral infiltration of glioma in rat
TU Yingshan  WENG Aiting  REN Anli  DONG Peng 

Cite this article as: TU Y S, WENG A T, REN A L, et al. Multimodal MRI manifestations and pathological basis of peritumoral infiltration of glioma in rat[J]. Chin J Magn Reson Imaging, 2023, 14(1): 105-110. DOI:10.12015/issn.1674-8034.2023.01.019.


[Abstract] Objective The characteristics of multimodal MRI in peritumoral infiltrating region of cerebral glioma and its correlation with corresponding pathological indicators were analyzed, so as to explore the molecularly biological basis of peritumoral infiltration in glioma.Materials and Methods A total of 32 female Wistar rats were selected as research subjects, which were injected C6 glioma cells to established rat glioma models in situ by micro sampling syringe. The control group was injected with the same amount of complete culture medium without glioma cells. The rats were examined with conventional MRI sequences, arterial spin labeling (ASL), diffusion weighted imaging (DWI), magnetic resonance spectrum (MRS) after 14 days. The relative apparent diffusion coefficient (rADC), Cho/NAA were measured and calculated, and the cerebral blood flow (CBF) was measured. At the same time, the expression of Ki-67, vascular endothelial growth factor (VEGF) and microvessel density (MVD) of CD105 were recorded. The correlation between the rADC value, CBF and Ki-67, VEGF, CD105-MVD were analyzed.Results (1) The differences in rADC, CBF, Ki-67, VEGF and CD105-MVD which between each two groups in the central region, the infiltrating region, the control group were statistically significant (P＜0.05); (2) The Cho/NAA in the central area of glioma was higher than that in control group (P＜0.05); (3) The rADC value were negatively correlated with Ki-67 in the central area of glioma and the infiltrating region (r=-0.92, -0.74); (4) The Cho/NAA was positively correlated with Ki-67 in the central area of glioma (r=0.76). There were positive correlations between the CBF and the expression of VEGF, CD105-MVD in the corresponding region (r=0.90, 0.72). The CBF in the infiltrating region had positive correlations with the expression of VEGF and CD105-MVD (r=0.90, 0.71).Conclusions This study revealed multimodal MRI was correlated with relative pathological indicators, and multimodal MRI was helpful to preliminary evaluate the molecularly biological characteristics in glioma and peritumoral infiltrating area, which may provide a certain basis for evaluation of tumoral scope and surgical excision.
[Keywords] cerebral glioma；peritumoral infiltration；magnetic resonance imaging；multimodal magnetic resonance imaging；arterial spin labeling；diffusion weighted imaging；magnetic resonance spectrum；rat；pathology

TU Yingshan1, 2   WENG Aiting2   REN Anli2   DONG Peng2*  

1 Department of Radiology, Fuzhou Second Hospital, Fuzhou, 350007, China

2 School of Medical Imaging, Weifang Medical University, Weifang 261053, China

Corresponding author: Dong P, E-mail: dongpeng01502@163.com

Conflicts of interest   None.

ACKNOWLEDGMENTS Natural Science Foundation of Shandong Province (No. ZR2014HL083).
Received  2022-06-22
Accepted  2022-11-29
DOI: 10.12015/issn.1674-8034.2023.01.019
Cite this article as: TU Y S, WENG A T, REN A L, et al. Multimodal MRI manifestations and pathological basis of peritumoral infiltration of glioma in rat[J]. Chin J Magn Reson Imaging, 2023, 14(1): 105-110. DOI:10.12015/issn.1674-8034.2023.01.019.

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