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• Original Article •
MRI tracer imaging explore the diffusion changes of brain extracellular space in a rat model of intracranial cryptococcal infection
WANG Gang  NUERBIYEMU·Abulikemu   ZHAO Jingjing  LI Yanran  LI Jin  JIANG Chunhui  HE Qingyuan  WANG Wei  WANG Jian 

Cite this article as: WANG G, NUERBIYEMU A B L K M, ZHAO J J, et al. MRI tracer imaging explore the diffusion changes of brain extracellular space in a rat model of intracranial cryptococcal infection[J]. Chin J Magn Reson Imaging, 2023, 14(2): 103-108. DOI:10.12015/issn.1674-8034.2023.02.018.


[Abstract] Objective To quantitatively analyze the signal intensity of the intracranial cryptococcal infection rat model by using MRI tracer imaging, and explore the changes of the tracer diffusion in the extracellular space of the infected rats brain.Materials and Methods A total of 30 adult SD rats were intracranial in situ inoculated with cryptococcus type A suspension to establish infection models, and 20 rats were set up as control group without any treatment. In the model group, MRI T2WI scanning was performed at 2, 3 and 4 weeks after modeling to monitor the establishment of the model, and the rats successfully modeled were included in the case group. The rats in the case and control group were microinjected with 10 mmol/L of gadolinium-diethylene triaminepentaacetic acid (Gd-DTPA). T1 weighted imaging three dimensional magnetization prepared rapid acquisition echo (T1WI 3D MP-RAGE) images of before (T0) and 15, 45, 90, 120, 180, 240 min after the injection were collected respectively. The diffusion characteristics of the tracer in the brain extracellular space (ECS) of the two groups were dynamically observed. x¯±s in maximum level signal strength statistic of the acquired images were extracted by ITK-SNAP software. Differences of standard deviation between case group and control group were compared. The mean, the mean of the standard deviation and their linear relation with time were taken to draw the time signal curve and the curve form and trend of the two groups were observed. The independent sample t test was used to compare the difference of mean between the two groups and the changes of ECS diffusion parameters λ and pathophysiological mechanisms were speculated.Results The success rate of modeling was 73.3%. The mean and standard deviation of intensity distribution of image obtained at 15 min after injection were the largest, and the mean and standard deviation of the case group was significantly higher than that of the control group. The mean and standard deviation of each time period followed the normal distribution, and the P-values of independent sample t-test at T0, T15, T45, T90, T120, T180 and T240 were 0.019, 0.048, 0.150, 0.878, 0.845, 0.603, 0.819 for mean and 0.285, 0.017, 0.327, 0.308, 0.891, 0.298, 0.486 for standard deviation.Conclusions The MRI T1WI 3D MP-RAGE signal intensity parameters can reveal the changes of ECS drainage in intracranial cryptococcus neoformans infection, and the mean and standard deviation of signal intensity at T15 can distinguish the changes of brain ECS in in intracranial cryptococcus neoformans infection.
[Keywords] rat；cryptococcus；central nervous system；extracellular space；tracer；signal intensity；magnetic resonance imaging

WANG Gang1, 2   NUERBIYEMU·Abulikemu 1   ZHAO Jingjing1   LI Yanran1   LI Jin1   JIANG Chunhui1   HE Qingyuan3   WANG Wei4   WANG Jian1*  

1 the First Affiliated Hospital of Xinjiang Medical University, Urumqi 830011, China

2 Medical Imaging Center, Xi'an GEM Flower Changqing Hospital, Xi'an 710000, China

3 Department of Radiology, the Third Hospital of Peking University, Beijing Key Laboratory of Magnetic Resonance Equipment and Technology, Beijing 100191, China

4 MR Room, Foshan First People's Hospital (Foshan Hospital Affiliated to Sun Yat-sen University), Foshan 528000, China

*Correspondence to: Wang J, E-mail: jeanw1265@163.com

Conflicts of interest   None.

ACKNOWLEDGMENTS National Natural Science Foundation of China (No. U1903121); Foshan Mountain Climbing Plan (No. 2019C016).
Received  2022-05-25
Accepted  2022-11-28
DOI: 10.12015/issn.1674-8034.2023.02.018
Cite this article as: WANG G, NUERBIYEMU A B L K M, ZHAO J J, et al. MRI tracer imaging explore the diffusion changes of brain extracellular space in a rat model of intracranial cryptococcal infection[J]. Chin J Magn Reson Imaging, 2023, 14(2): 103-108. DOI:10.12015/issn.1674-8034.2023.02.018.

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