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• Original Article •
Study of conventional MRI combined with SWI on brain damage after asphyxia in neonatal rats and compared with pathological
XIE Beichen  YAN Ruifang  REN Jipeng  NIU Jin  LI Haiming  DU Chaoyang 

Cite this article as: XIE B C, YAN R F, REN J P, et al. Study of conventional MRI combined with SWI on brain damage after asphyxia in neonatal rats and compared with pathological[J]. Chin J Magn Reson Imaging, 2023, 14(7): 108-114. DOI:10.12015/issn.1674-8034.2023.07.019.


[Abstract] Objective To investigate the pathological types of hypoxic brain injury in neonatal rats after asphyxia by conventional magnetic resonance imaging (MRI) and susceptibility-weighted imaging (SWI), and to explore the effects of hypoxia on the cortex and hippocampus.Materials and Methods Neonatal 7-day-old rats were randomly divided into asphyxiation and control groups. Pups in the asphyxiation group were placed in a closed chamber for a hypoxia with the oxygen concentration with 1% (5 min)-reoxygenation with the oxygen concentration of 21% (3 min) cycle for a cumulative hypoxia time of 30 min; control rats were placed in the same chamber with air for the same time. Behavioral changes and changes in general conditions of baby rats during and after hypoxia were observed during asphyxia. Abnormal behavioral observations were made and recorded at 1 d, 3 d and 7 d after hypoxia. T1WI, T2WI and SWI sequences were performed at 1 d, 3 d and 7 d after modelling. Hematoxylin-Eosin staining was performed according to the site of abnormal MRI signal to observe the type of pathological injury in the abnormal signal area. Neuronal survival in the cortical, dentate grrus (DG), CA1 (corn ammonis 1) and CA3 (corn ammonis 3) regions of the hippocampus of young rats was observed by Nissl staining; the expression of apoptosis-related protein Caspase-3 was detected by immunohistochemical staining.Results Seizures were observed in pups at 1 d, 3 d and 7 d after hypoxia. MRI and SWI showed types of brain injury including enlarged ventricles, foci of cerebral softening, intraventricular and parenchymal hemorrhage and microvascular dilatation. Nissl staining showed a decrease in neurons in the cortical and hippocampal DG regions at 1 d, 3 d and 7 d after hypoxia compared to the control group (P＜0.05), and a decrease in neurons in the CA3 region at 7 d after hypoxia (P＜0.05). Immunohistochemical staining showed that the expression of Caspase-3 in the DG region of the hippocampus was higher than that of the control group at 1 d, 3 d and 7 d after hypoxia (P＜0.05), and the expression in the cortex was higher than that of the control group at 3 d and 7 d after hypoxia (P＜0.05); the expression in the CA1 and CA3 regions of the hippocampus was higher than that of the control group at 7 d after hypoxia (P＜0.05).Conclusions MRI and SWI show that brain damage can be present early in acute intermittent hypoxia and that hypoxic brain damage can lead to seizures. This suggests that MRI and SWI should be used as routine clinical investigations in children with a history of asphyxia and suspected brain injury, and that early clinical intervention should be undertaken.
[Keywords] hypoxia；brain injury；seizure；neonatal rats；magnetic sensitivity-wighted imaging；magnetic resonance imaging

XIE Beichen1   YAN Ruifang1*   REN Jipeng1   NIU Jin1, 2   LI Haiming2   DU Chaoyang2  

1 Department of Magnetic Resonance, the First Affiliated Hospital of Xinxiang Medical University, Xinxiang 453100, China

2 Henan Provincial Key Laboratory of Neurorepair, the First Affiliated Hospital of Xinxiang Medical University, Xinxiang 453100, China

Corresponding author: Yan RF, E-mail: yrf718@163.com

Conflicts of interest   None.

Received  2023-02-10
Accepted  2023-06-28
DOI: 10.12015/issn.1674-8034.2023.07.019
Cite this article as: XIE B C, YAN R F, REN J P, et al. Study of conventional MRI combined with SWI on brain damage after asphyxia in neonatal rats and compared with pathological[J]. Chin J Magn Reson Imaging, 2023, 14(7): 108-114. DOI:10.12015/issn.1674-8034.2023.07.019.

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