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• Original Article •
Value of intravoxel incoherent motion magnetic resonance imaging in evaluating renal fibrosis in rabbits with renal artery stenosis
ZHA Tingting  CHEN Jie  YU Shengnan  LIU Guoqiang  CHEN Jing  XING Wei 

Cite this article as: ZHA T T, CHEN J, YU S N, et al. Value of intravoxel incoherent motion magnetic resonance imaging in evaluating renal fibrosis in rabbits with renal artery stenosis[J]. Chin J Magn Reson Imaging, 2023, 14(11): 128-135. DOI:10.12015/issn.1674-8034.2023.11.021.


[Abstract] Objective To investigate the value of MR intravoxel incoherent motion imaging (IVIM) in evaluating renal fibrosis (RF) in rabbits with renal artery stenosis.Materials and Methods Seventy-eight healthy New Zealand white rabbits were randomly divided into control group (n=8) and RF group (n=70). The RF group was further randomly divided into six subgroups: pre-RF、RF-1W、RF-2W、RF-3W、RF-4W subgroup (n=12 in each subgroup), and RF-longitude (RF-L) subgroup (n=10) for dynamic observation. The rabbits in the RF group underwent left renal artery stenosis to establish the RF model. MR axial T2WI and IVIM scans were performed before operation and at 1, 2, 3 and 4 weeks after operation, respectively. After the last MR scan, the left kidney was resected and histopathological examination was performed. The rabbits in the RF-L subgroup and the control group underwent MR scanning at each time point and histopathological examinations at the end of the scanning at 4 weeks after operation. The true diffusion coefficient (D)、pseudo-diffusion coefficient (D*) and perfusion fraction (f) values of bilateral renal cortex and medulla were measured and calculated on IVIM images. Repeated measures analysis of variance was used to compare the differences of D, D* and f values in the left and right renal cortex and medulla over time in each subgroup. Independent sample t test was used to compare the differences of D, D* and f values of bilateral renal cortex and medulla at each time point. Spearman correlation analysis was used to compare the correlation between the D, D*, f values of the left renal cortex and medulla and the degree of RF. Receiver operating characteristic (ROC) curve was used to determine the efficacy of D, D*, f values of left renal cortex and medulla in the diagnosis and staging of RF.Results In the RF group, the D and f values of left renal cortex and medulla decreased gradually with RF. The D* values of left renal cortex were significantly different between RF-4W and pre-RF, RF-1W, RF-2W subgroups (P all＜0.05). However, the D* values of the left renal medulla did not differ significantly over time. The D* and f values of the right renal cortex and medulla gradually increased with RF. However, the D values of the right kidney cortex and medulla did not differ significantly over time. In the control group, there was no significant difference in IVIM parameters between left and right renal cortex and medulla over time. The D and D* values of the left renal cortex and medulla in the RF-4W subgroup were statistically different (P all <0.05). The f values of the left renal cortex and medulla in the pre-RF, RF-3W and RF-4W subgroups were statistically different (P all <0.05). The f values of the right renal cortex and medulla in the pre-RF and RF-4W subgroups were statistically different (P all <0.05). Spearman correlation test showed that the D and f values of left renal cortex and the f value of left renal medulla were moderately or strongly negatively correlated with the degree of RF (r=-0.595, -0.717, -0.412, P all <0.01). The D* values of left renal cortex and the D、D* values of the left renal medulla were not correlated with the degree of RF (P all >0.05). The f value was the best to identify pre-RF and RF-1W-RF-4W, pre-RF-RF-1W and RF-2W-RF-4W. The D value was the best to identify pre-RF-RF-2W and RF-3W-RF-4W, pre-RF-RF-3W and RF-4W.Conclusions MR-IVIM can reflect the occurrence and development of RF from two aspects of perfusion and diffusion respectively. It has a great value in evaluating and staging RF.
[Keywords] renal fibrosis；renal artery stenosis；intravoxel incoherent motion；magnetic resonance imaging

ZHA Tingting   CHEN Jie   YU Shengnan   LIU Guoqiang   CHEN Jing   XING Wei*  

Department of Radiology, Third Affiliated Hospital of Soochow University, Changzhou 213003, China

Corresponding author: XING W, E-mail: suzhxingwei@suda.edu.cn

Conflicts of interest   None.

ACKNOWLEDGMENTS Major Sci & Tech Program of Changzhou Health Bureau (No. ZD202211); Young Talent Development Plan of Changzhou Health Commission (No. CZQM2020037); Project of State Key Laboratory of Radiation Medicine and Protection, Soochow University (No. GZK12023046 ).
Received  2023-08-01
Accepted  2023-10-27
DOI: 10.12015/issn.1674-8034.2023.11.021
Cite this article as: ZHA T T, CHEN J, YU S N, et al. Value of intravoxel incoherent motion magnetic resonance imaging in evaluating renal fibrosis in rabbits with renal artery stenosis[J]. Chin J Magn Reson Imaging, 2023, 14(11): 128-135. DOI:10.12015/issn.1674-8034.2023.11.021.

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