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• Original Article •
Application value of glutamate chemical exchange saturation transfer imaging in radiation-induced brain injury rat
LI Guodong  LIU Yan  LI Hao  LIU Caiyun  LI Xianglin  LI Qinglong  WENG Na  BIN Li  HUANG Danqi  WANG Xu 

Cite this article as: LI G D, LIU Y, LI H, et al. Application value of glutamate chemical exchange saturation transfer imaging in radiation-induced brain injury rat[J]. Chin J Magn Reson Imaging, 2023, 14(12): 85-90, 97. DOI:10.12015/issn.1674-8034.2023.12.014.


[Abstract] Objective To investigate the value of 7.0 T magnetic resonance glutamate chemical exchange saturation transfer (GluCEST) imaging for assessing hippocampal glutamate changes in a rat model of radiation brain injury.Materials and Methods The experimental rats were randomly divided into control group (n=9) and radiation-induced brain injury (RBI) group (n=9). Rats in the RBI group were irradiated using an X-ray irradiator and subjected to a water maze experiment 4 weeks later. Chemical exchange saturation transfer (CEST) sequences were collected using 7.0 T small animal magnetic resonance and hippocampal glutamate concentrations were measured by high-performance liquid chromatography (HPLC). The t-tests were performed for differences in GluCEST and glutamate concentrations in the hippocampal brain regions between the two groups. Pearson correlation analyses were performed for the relationship between GluCEST and HPLC and between GluCEST and water maze.Results Compared to control group, rats in the RBI group had impaired cognitive function and reduced hippocampal GluCEST (P=0.008) after 4 weeks, and reduced glutamate levels were confirmed by HPLC measurements (P=0.003). Correlation analysis showed a positive correlation between glutamate concentration by HPLC and GluCEST (r=0.71, P＜0.001), and spatial memory also showed a consistent positive correlation with hippocampal GluCEST (r=0.50, P=0.034).Conclusions GluCEST imaging visualizes and evaluates hippocampal glutamate changes of RBI rats and might be used as an imaging biomarker for metabolic changes in vivo, which might be useful for the monitoring and intervention of concomitant radiation damage after radiotherapy.
[Keywords] radiation brain injury；cognitive impairment；radiation therapy；chemical exchange saturation transfer；magnetic resonance imaging

LI Guodong1   LIU Yan2   LI Hao2   LIU Caiyun1   LI Xianglin2   LI Qinglong3   WENG Na1   BIN Li1   HUANG Danqi1   WANG Xu1*  

1 Department of Nuclear Medicine, Binzhou Medical University Hospital, Binzhou 256600, China

2 School of Medical Imaging, Binzhou Medical University, Yantai 264003, China

3 Department of Magenetic Resonance Imaging, Henan Province Hospital of Traditional Chinese Medicine, Zhengzhou 450000, China

Corresponding author: WANG X, E-mail: wangxu1978@163.com#Co first author: LIU Y

Conflicts of interest   None.

ACKNOWLEDGMENTS National Natural Science Foundation of China (No. 81771828, 11805247).
Received  2023-06-05
Accepted  2023-11-24
DOI: 10.12015/issn.1674-8034.2023.12.014
Cite this article as: LI G D, LIU Y, LI H, et al. Application value of glutamate chemical exchange saturation transfer imaging in radiation-induced brain injury rat[J]. Chin J Magn Reson Imaging, 2023, 14(12): 85-90, 97. DOI:10.12015/issn.1674-8034.2023.12.014.

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