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Progress in the application of magnetic resonance spectroscopy in Chinese medicine research
WU Shuang  LÜ Zhizhen  ZHOU Xingchen  LI Tao  HONG Ze  SUN Jiayu  LÜ Lijiang 

Cite this article as: WU S, LÜ Z Z, ZHOU X C, et al. Progress in the application of magnetic resonance spectroscopy in Chinese medicine research[J]. Chin J Magn Reson Imaging, 2024, 15(4): 229-234. DOI:10.12015/issn.1674-8034.2024.04.038.


[Abstract] Magnetic resonance spectroscopy (MRS), a spectroscopic technique that applies magnetic resonance data to the determination of molecular structure, provides an innovative research tool for the study and development of mechanisms in the field of traditional Chinese medicine (TCM), which helps to deepen the modern understanding of TCM theories and promotes the continued development of the TCM discipline. The MRS technique allows the detection of metabolites in the brain and the in vitro identification of unknown compounds present in body fluids or tissues, revealing the central brain mechanisms under the intervention of TCM tools. This study focused on the overview of MRS technology, the advantages of combining MRS technology with the field of Chinese medicine, the current status of research on the application of MRS technology in the field of Chinese medicine to summarise, and to explore the gaps and shortcomings in the previous research on the application of MRS technology in the field of Chinese medicine, with a view to providing useful references for researchers.
[Keywords] magnetic resonance spectroscopy;traditional Chinese medicine;applied research;action mechanism;magnetic resonance imaging;cerebral metabolism

WU Shuang1, 2   LÜ Zhizhen1, 2   ZHOU Xingchen1, 2   LI Tao1, 2   HONG Ze1   SUN Jiayu1   LÜ Lijiang1, 2*  

1 Department of Tuina, the Third Affiliated Hospital of Zhejiang Chinese Medical University (Zhongshan Hospital of Zhejiang Province), Hangzhou 310005, China

2 The Third School of Clinical Medicine, Zhejiang Chinese Medical University, Hangzhou 310053, China

Corresponding author: LÜ L J, E-mail: lvlijiang0288@163.com

Conflicts of interest   None.

Received  2023-12-27
Accepted  2024-04-07
DOI: 10.12015/issn.1674-8034.2024.04.038
Cite this article as: WU S, LÜ Z Z, ZHOU X C, et al. Progress in the application of magnetic resonance spectroscopy in Chinese medicine research[J]. Chin J Magn Reson Imaging, 2024, 15(4): 229-234. DOI:10.12015/issn.1674-8034.2024.04.038.

[1]
DENG J, HE J B, QIU L H. Research progress of magnetic resonance functional brain imaging in adolescent depression[J]. Chin J Magn Reson Imag, 2022, 13(8): 101-103, 108. DOI: 10.12015/issn.1674-8034.2022.08.022.
[2]
HUANG M S, PENG Y W, HE Q D, et al. NMR-based metabonomics reveals the dynamic effect of electro-acupuncture on central nervous system in gastric mucosal lesions (GML) rats[J/OL]. Chin Med, 2022, 17(1): 37 [2023-11-26]. https://pubmed.ncbi.nlm.nih.gov/35313919/. DOI: 10.1186/s13020-022-00593-9.
[3]
ZHOU X C, LV Z Z, HUANG Y B, et al. Application of multimodal MRI and massage intervention in lumbar disc herniation[J/OL]. Chin J Integr Tradit West Med, 2023 [2023-12-16]. https://kns.cnki.net/kcms2/article/abstract?v=wcPNn8Zia7NtKj9Sh2v_K2mWrhZIbO4znolIzOTb1tBz-WcHEresNSOyIDZLsSHCu4GUlXNcd9YsZNFnpcSHhT1XbMTvwT2OIYPU4WKsvVqJU8y8bwN5SFoeo9AUz1YP0rbtamRAU_M=&uniplatform=NZKPT&language=CHS. DOI: 10.7661/j.cjim.20231025.333.
[4]
YI M, DAI X P, LI Q X, et al. 1H-NMR-based metabolomics study on coronary heart disease with blood-stasis syndrome and phlegm syndrome[J]. J Cent South Univ Med Sci, 2021, 46(6): 591-600. DOI: 10.11817/j.issn.1672-7347.2021.190172.
[5]
WEI Y F, CHEN L, LIU M, et al. Effects of Zishen Yisui Formula on the expression of major metabolites of substantia nigra in Parkinson's disease patients with deficiency of marrow-reservoir by magnetic resonance spectroscopy[J]. China J Tradit Chin Med Pharm, 2018, 33(5): 2193-2197.
[6]
LIN R H, LI L, ZHANG Y Z, et al. Electroacupuncture ameliorate learning and memory by improving N-acetylaspartate and glutamate metabolism in APP/PS1 mice[J/OL]. Biol Res, 2018, 51(1): 21 [2023-12-24]. https://pubmed.ncbi.nlm.nih.gov/29980225/. DOI: 10.1186/s40659-018-0166-7.
[7]
DIDEHDAR D, KAMALI F, YOOSEFINEJAD A K, et al. The effect of spinal manipulation on brain neurometabolites in chronic nonspecific low back pain patients: a randomized clinical trial[J]. Ir J Med Sci, 2020, 189(2): 543-550. DOI: 10.1007/s11845-019-02140-2.
[8]
HUO M, WANG W, YANG C Y, et al. Dynamic changes of central metabolites in chronic low back pain after intervening with Tuina monitored by proton magnetic resonance spectroscopy[J]. Chin J Med Imag, 2022, 30(4): 305-311. DOI: 10.3969/j.issn.1005-5185.2022.04.001.
[9]
GAO N. A study on the analgesic mechanism of acupuncture stimulation in knee osteoarthritis based on MRS and fMRI[D].Hefei: University of Science and Technology of China, 2022. DOI: 10.27517/d.cnki.gzkju.2022.001397.
[10]
LI R, CHANG X, ZHANG J, et al. Progress of magnetic resonance spectroscopy in the study of the effects of smoking on the brain[J]. Chin J Magn Reson, 2023, 40(4): 471-480. DOI: 10.11938/cjmr20233052.
[11]
WU S, LÜ Z Z, ZHOU X C, et al. Research progress of MRS in evaluating brain metabolites and neurotransmitters in patients with lumbar disc herniation[J]. Zhejiang J Integr Tradit Chin West Med, 2024, 34(2): 186-189. DOI: 10.3969/j.issn.1005-4561.2024.02.024.
[12]
LIANG R H, ZHANG S P, XIE Y H. Study about influence of brain metabolism in patients with chronic migraine after acupuncture at Shaoyang specific acupoints[J]. Chin Arch Tradit Chin Med, 2016, 34(4): 918-920. DOI: 10.13193/j.issn.1673-7717.2016.04.045.
[13]
LONG T. The study on the metabolites in Insula of rats with functional dyspepsia upon the impact of acupuncture[D].Chengdu: Chengdu University of TCM, 2019.
[14]
ZOU Z J, LIU Z H, GONG M J, et al. Intervention effects of puerarin on blood stasis in rats revealed by a (1)H NMR-based metabonomic approach[J]. Phytomedicine, 2015, 22(3): 333-343. DOI: 10.1016/j.phymed.2015.01.006.
[15]
DAI Y T, LI Q, TONG J Y, et al. Quality marker identification based on standard decoction of differently processed materials of Ephedrae Herba[J/OL]. J Ethnopharmacol, 2019, 237: 47-54 [2023-12-26]. https://pubmed.ncbi.nlm.nih.gov/30898554/. DOI: 10.1016/j.jep.2019.03.025.
[16]
LI W Z, ZHAO F, YANG J Y, et al. Development of a comprehensive method based on quantitative 1H NMR for quality evaluation of Traditional Chinese Medicine injection: a case study of Danshen Injection[J]. J Pharm Pharmacol, 2022, 74(7): 1006-1016. DOI: 10.1093/jpp/rgac034.
[17]
LI C Y, XU H X, HAN Q B, et al. Quality assessment of Radix Codonopsis by quantitative nuclear magnetic resonance[J]. J Chromatogr A, 2009, 1216(11): 2124-2129. DOI: 10.1016/j.chroma.2008.10.080.
[18]
ZHANG H S. Study on effects of fMRI and BGP in DSH PI with abdominal massage based on "brain-bowel interaction"[D].Changchun: Changchun University of Chinese Medicine, 2020. DOI: 10.26980/d.cnki.gcczc.2020.000004.
[19]
TENSAOUTI F, DESMOULIN F, GILHODES J, et al. Quality control of 3D MRSI data in glioblastoma: can we do without the experts?[J]. Magn Reson Med, 2022, 87(4): 1688-1699. DOI: 10.1002/mrm.29098.
[20]
CAKMAKCI D, KAYNAR G, BUND C, et al. Targeted metabolomics analyses for brain tumor margin assessment during surgery[J]. Bioinformatics, 2022, 38(12): 3238-3244. DOI: 10.1093/bioinformatics/btac309.
[21]
SUO H N, LI Y C, PENG R C. Application of 1H-MRS and DTI in the differential diagnosis of high-grade gliomas and metastatic tumors[J]. Chin J Magn Reson Imag, 2023, 14(12): 103-108, 120. DOI: 10.12015/issn.1674-8034.2023.12.017.
[22]
SUN Y, ZOU Y F, HAO Y F, et al. Research on application of magnetic resonance Histo sequence in fat quantification of shoulder joint supraspinatus muscle[J]. J Med Res Combat Trauma Care, 2023, 36(5): 488-492. DOI: 10.16571/j.cnki.2097-2768.2023.05.008.
[23]
MA F H, ZHANG G F, QIANG J W, et al. Study of MR spectroscopy and diffusion weighted imaging in differentiating benign from malignant uterine tumors[J]. Chin Comput Med Imag, 2018, 24(4): 316-320. DOI: 10.19627/j.cnki.cn31-1700/th.2018.04.009.
[24]
PIMENTEL-SILVA L R, CASSEB R F, CORDEIRO M M, et al. Interactions between in vivo neuronal-glial markers, side of hippocampal sclerosis, and pharmacoresponse in temporal lobe epilepsy[J]. Epilepsia, 2020, 61(5): 1008-1018. DOI: 10.1111/epi.16509.
[25]
PATKEE P A, BABURAMANI A A, LONG K R, et al. Neurometabolite mapping highlights elevated myo-inositol profiles within the developing brain in down syndrome[J/OL]. Neurobiol Dis, 2021, 153: 105316 [2023-12-26]. https://pubmed.ncbi.nlm.nih.gov/34865940/. DOI: 10.1016/j.nbd.2021.105316.
[26]
MORENO-BRAUER D, HÄUSLER M, KLUGER G, et al. Spectrum, evolution, and clinical relationship of magnetic resonance imaging in 31 children with febrile infection-related epilepsy syndrome[J]. Neuropediatrics, 2024, 55(1): 9-15. DOI: 10.1055/s-0043-1774318.
[27]
MU S K, WANG J X, GONG S Y. Application of medical imaging based on deep learning in the treatment of lumbar degenerative diseases and osteoporosis with bone cement screws[J/OL]. Comput Math Methods Med, 2021, 2021: 2638495 [2023-12-24]. https://pubmed.ncbi.nlm.nih.gov/34671416/. DOI: 10.1155/2021/2638495.
[28]
SHU Y Q, HE L C. Progresses of magnetic resonance imaging in cervical spondylotic myelopathy[J]. Int J Med Radiol, 2021, 44(2): 202-206. DOI: 10.19300/j.2021.Z18336.
[29]
NELSON E A, KRAGULJAC N V, MAXIMO J O, et al. Hippocampal dysconnectivity and altered glutamatergic modulation of the default mode network: a combined resting-state connectivity and magnetic resonance spectroscopy study in schizophrenia[J]. Biol Psychiatry Cogn Neurosci Neuroimaging, 2022, 7(1): 108-118. DOI: 10.1016/j.bpsc.2020.04.014.
[30]
DEMAYO M M, HARRIS A D, SONG Y J C, et al. Age-related parietal GABA alterations in children with autism spectrum disorder[J]. Autism Res, 2021, 14(5): 859-872. DOI: 10.1002/aur.2487.
[31]
KORENIC S A, KLINGAMAN E A, WICKWIRE E M, et al. Sleep quality is related to brain glutamate and symptom severity in schizophrenia[J/OL]. J Psychiatr Res, 2020, 120: 14-20 [2023-12-26]. https://pubmed.ncbi.nlm.nih.gov/31610406/. DOI: 10.1016/j.jpsychires.2019.10.006.
[32]
TAN Z H, LIU H H, PAN H S, et al. Characteristics of magnetic resonance spectroscopy pops in different TCM syndromes of vascular dementia[J]. Clin J Tradit Chin Med, 2017, 29(8): 1140-1143. DOI: 10.16448/j.cjtcm.2017.0381.
[33]
QI W, WU C H, XU Y Y, et al. Ischemic stroke magnetic resonance spectrum analysis and the preliminary research on the relationship between TCM syndrome type[J]. J Fujian Univ Tradit Chin Med, 2011, 21(6): 7-9. DOI: 10.13261/j.cnki.jfutcm.002566.
[34]
ZHANG L P, ZHANG H, SHI J, et al. Relationship between TCM syndromes and changes of 1H-MRS of mild cognitive impairment[J]. J Beijing Univ Tradit Chin Med, 2011, 34(5): 353-357, 360.
[35]
LI J, GUO Z H, LIU J H, et al. Study on metabonomics of myocardial tissue of rat model with coronary heart disease of heart blood stasis syndrome[J]. Chin J Inf Tradit Chin Med, 2024, 31(3): 119-126. DOI: 10.19879/j.cnki.1005-5304.202304482.
[36]
CUI Y, GUO H, KUANG H X, et al. Overview on application of metabonomics in study on traditional Chinese medicine syndromes[J]. China J Tradit Chin Med Pharm, 2017, 32(2): 672-675.
[37]
MA S N, GUAN Y Q, ZHANG M, et al. The advantage of metabolomics in the study of TCM syndrome[J]. Lishizhen Med Mater Med Res, 2019, 30(7): 1714-1716. DOI: 10.3969/j.issn.1008-0805.2019.07.064.
[38]
LIU Z Y, ZHAN X H, LIU Y, et al. Effects of Xiaoyao pill on magnetic resonance spectroscopy of mild cognitive impairment patients with liver qi stagnation syndrome[J]. J Tradit Chin Med, 2018, 59(17): 1489-1493. DOI: 10.13288/j.11-2166/r.2018.17.012.
[39]
PAN L N, DENG Y H, FAN P, et al. Study on efficacy evaluation of proton magnetic resonance spectroscopy for cerebral infarction with Qi deficiency and blood stasis[J]. Lishizhen Med Mater Med Res, 2019, 30(9): 2277-2279. DOI: 10.3969/j.issn.1008-0805.2019.09.079.
[40]
HUANG Y, ZHANG Z X, CHEN R H, et al. Clinical effect of Wenzhen Yunqi Recipe on the negative emotion and hippocampus functional metabolism in patients with chronic fatigue syndrome[J]. Shanghai J Tradit Chin Med, 2016, 50(10): 60-63. DOI: 10.16305/j.1007-1334.2016.10.017.
[41]
ZHAO Z J, ZHANG W, ZHANG Y, et al. Multimodal magnetic resonance imaging and therapeutic intervention with yi-Nao-Jie-yu decoction in a rat model of post-stroke depression[J/OL]. Front Psychiatry, 2020, 11: 557423 [2023-12-26]. https://pubmed.ncbi.nlm.nih.gov/33329096/. DOI: 10.3389/fpsyt.2020.557423.
[42]
GUAN Q X, LIANG S W, WANG Z H, et al. ¹H NMR-based metabonomic analysis of the effect of optimized rhubarb aglycone on the plasma and urine metabolic fingerprints of focal cerebral ischemia-reperfusion rats[J]. J Ethnopharmacol, 2014, 154(1): 65-75. DOI: 10.1016/j.jep.2014.03.002.
[43]
ZHENG H T, LI K N, CHEN C, et al. Effects of Renshenjian Decoction on pancreatic metabonomic profiles in insulin resistance rats based on ¹H-NMR metabonomics[J]. Zhongguo Zhong Yao Za Zhi, 2018, 43(14): 3012-3017. DOI: 10.19540/j.cnki.cjcmm.20180327.003.
[44]
SONG C L. Study on the intervention effect of liver-sparing, heat-clearing and spleen-strengthening method on brain metabolism in rats with environment-related fear model[D]. Beijing: Beijing University of Chinese Medicine, 2014.
[45]
HUNG H Y, LIN S M, LI C Y, et al. A rapid and feasible 1H-NMR quantification method of ephedrine alkaloids in Ephedra herbal preparations[J/OL]. Molecules, 2021, 26(6): 1599 [2023-12-26]. https://pubmed.ncbi.nlm.nih.gov/33805790/. DOI: 10.3390/molecules26061599.
[46]
SUN L M, YAN H, ZHANG Y. Magnetic resonance spectroscopy (MRS) of multifidus muscle metabolites in chronic low back pain (CLBP)[J]. Eur Spine J, 2023, 32(12): 4397-4404. DOI: 10.1007/s00586-023-07933-9.
[47]
CHENG X, WANG D M, CHEN X, et al. Health Qigong Wuqinxi improves hydrogen proton magnetic resonance spectra in prefrontal cortex and hippocampus in college students with mild depression[J]. J South Med Univ, 2016, 36(11): 1468-1476. DOI: 10.3969/j.issn.1673-4254.2016.11.04.
[48]
PIGONI A, DELVECCHIO G, SQUARCINA L, et al. Sex differences in brain metabolites in anxiety and mood disorders[J]. Psychiatry Res Neuroimaging, 2020, 305: 111196. DOI: 10.1016/j.pscychresns.2020.111196.
[49]
COLLET S, BHADURI S, KIYAR M, et al. Characterization of the 1H-MRS metabolite spectra in transgender men with gender Dysphoria and cisgender people[J/OL]. J Clin Med, 2021, 10(12): 2623 [2023-12-26]. https://pubmed.ncbi.nlm.nih.gov/34198690/. DOI: 10.3390/jcm10122623.
[50]
ZHAO Y P, FANG J L, CHEN Y Y, et al. Magnetic resonance spectroscopy study on gender differences in the effects of acupuncture on prefrontal neurotransmitters in healthy volunteers[J]. Shanghai J Acupunct Moxibustion, 2023, 42(2): 211-217. DOI: 10.13460/j.issn.1005-0957.2023.02.0211.
[51]
WANG J J, ZHENG H T, HUA J, et al. Application of multimodal fMRI in studies of clinical efficacy of Chinese medicine: systematic review[J]. Chin Imag J Integr Tradit West Med, 2020, 18(1): 14-18. DOI: 10.3969/j.issn.1672-0512.2020.01.004.
[52]
WASSING R, LAKBILA-KAMAL O, RAMAUTAR J R, et al. Restless REM sleep impedes overnight amygdala adaptation[J]. Curr Biol, 2019, 29(14): 2351-2358.e4. DOI: 10.1016/j.cub.2019.06.034.
[53]
YU S Y, SHEN Z F, LAI R, et al. The orbitofrontal cortex gray matter is associated with the interaction between insomnia and depression[J]. Front Psychiatry, 2018, 9: 651. DOI: 10.3389/fpsyt.2018.00651.
[54]
CHEN L M, SHAO Z Q, LEI W R, et al. Abnormal hippocampal substructure volume in insomnia disorder[J]. Brain Imag Behav, 2022, 16(2): 672-679. DOI: 10.1007/s11682-021-00540-6.
[55]
SUN J J, LIU X M, SHEN C Y, et al. Reduced prefrontal activation during verbal fluency task in chronic insomnia disorder: a multichannel near-infrared spectroscopy study[J/OL]. Neuropsychiatr Dis Treat, 2017, 13: 1723-1731 [2023-12-26]. https://pubmed.ncbi.nlm.nih.gov/28721053/. DOI: 10.2147/ndt.s136774.
[56]
GUADAGNI V, BURLES F, FERRARA M, et al. Sleep quality and its association with the insular cortex in emotional empathy[J]. Eur J Neurosci, 2018, 48(6): 2288-2300. DOI: 10.1111/ejn.14124.
[57]
WOODWORTH D C, HOLLY L T, MAYER E A, et al. Alterations in cortical thickness and subcortical volume are associated with neurological symptoms and neck pain in patients with cervical spondylosis[J]. Neurosurgery, 2019, 84(3): 588-598. DOI: 10.1093/neuros/nyy066.
[58]
LÓPEZ-SOLÀ M, WOO C W, PUJOL J, et al. Towards a neurophysiological signature for fibromyalgia[J]. Pain, 2016, 158(1): 34-47. DOI: 10.1097/j.pain.0000000000000707.
[59]
XU H, ZHOU H, ZHOU Y F, et al. Research progress on brain center remodeling mechanism of Tuina based on fMRI technology in chronic pain[J]. China J Tradit Chin Med Pharm, 2023, 38(6): 2762-2765.

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