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• Original Article •
An experimental study of 3.0 T magnetic resonance imaging to evaluate autoimmune prostatitis in rats
CHEN Ziqi  LI Yize  SHAN Chunhui  YU Xiuqiao  ZHANG Luyao  WANG Zhenxian  CHEN Yingmin 

Cite this article as: CHEN Z Q, LI Y Z, SHAN C H, et al. An experimental study of 3.0 T magnetic resonance imaging to evaluate autoimmune prostatitis in rats[J]. Chin J Magn Reson Imaging, 2025, 16(2): 100-106. DOI:10.12015/issn.1674-8034.2025.02.016.


[Abstract] Objective To explore the application value of 3.0 T MRI in evaluating the severity of experimental autoimmune prostatitis (EAP) and the effect of zinc supplementation in rats.Materials and Methods This study was a prospective randomized controlled experiment, and five groups of rat models were constructed based on the traditional modeling method of autoimmune prostatitis rats, which were normal control (NC) group (6 rats), model group A (EAP-A group) (6 rats), treatment group A (EAPZ-A group) (6 rats), model group B (EAP-B group) (6 rats), and treatment group B (EAPZ-B group) (6 rats). Among them, the rats in the EAP-A group and the EAPZ-A group were modeled by intradermal injection of 15 mg/mL allogeneic prostate antigen mixture, the rats in the EAP-B group and the EAPZ-B group were modeled by intradermal injection of 30 mg/mL allogeneic prostate antigen mixture, and the rats in the NC group were modeled by intradermal injection of the same amount of normal saline. MRI scans were performed before the model was constructed (12 weeks old), after the model was constructed (18 weeks old) and after the treatment group was supplemented with zinc (22 weeks old), including axial T1WI, T2WI, diffusion weighted image (DWI) and sagittal proton density-weighted imaging (PDWI). At the end of the 22-week-old scan, the samples were collected and observed by pathological staining of hematoxylin-eosin (HE). The standardized T2WI signal intensity and average diffusion coefficient (ADC) values of the prostate gland at different weeks in each group were measured, and the prostate was compared between and within groups. Among them, one-way ANOVA was used to compare the standardized T2WI signal intensity of the prostate gland at different weeks in each group, and the least significant difference (LSD) was further compared when P < 0.05. The paired t-test was used for intra-group comparison of standardized T2WI signal intensity of rat prostate at different weeks of age in each group. One-way ANOVA was used to compare the ADC values of the prostate gland at different weeks in each group. The paired t-test was used for intra-group comparison of ADC values of prostate at different weeks in each group.Results At 12 weeks of age, there was no statistically significant difference in the intensity of standardized T2WI signal between the groups (P = 0.918). After successful modeling at 18 weeks of age, except for the NC group, the standardized T2WI signal intensity in the other four groups decreased to varying degrees, and the standardized T2WI signal intensity in the EAP-A group/EAPZ-A group was higher than that in the EAP-B group/EAPZ-B group (all P < 0.001), but there was no significant difference between EAP-A and EAPZ-A and EAPZ-B and EAPZ-B (P values were 0.340 and 0.113, respectively). After the end of treatment at 22 weeks, the standardized T2WI signal intensity in EAPZ-A and EAPZ-B groups rebounded, and the difference was not statistically significant (all P > 0.05), and they were all higher than those in EAP-A and EAP-B (all P < 0.001). There was no significant difference in the ADC values between groups and within groups at different weeks (all P > 0.05).Conclusions 3.0 T MRI can effectively assess the severity of EAP in rats, and can be used as a non-invasive means to monitor the effect of zinc supplementation, which has important clinical application potential.
[Keywords] experimental autoimmune prostatitis；Wistar rat model；magnetic resonance imaging；zinc；evaluation of efficacy

CHEN Ziqi1, 2   LI Yize2, 3   SHAN Chunhui3   YU Xiuqiao2, 4   ZHANG Luyao2, 3   WANG Zhenxian1*   CHEN Yingmin3*  

1 Department of Urology, Hebei General Hospital, Shijiazhuang 050051, China

2 Graduate School of Hebei Medical University, Shijiazhuang 050017, China

3 Department of Medical Imaging, Hebei General Hospital, Shijiazhuang 050051, China

4 Department of Ultrasound Medicine, Hebei General Hospital, Shijiazhuang 050051, China

Corresponding author: WANG Z X, E-mail: zhenxianw2022@163.com CHEN Y M, E-mail: hbghyingxiang@126.com

Conflicts of interest   None.

Received  2024-09-30
Accepted  2025-02-10
DOI: 10.12015/issn.1674-8034.2025.02.016
Cite this article as: CHEN Z Q, LI Y Z, SHAN C H, et al. An experimental study of 3.0 T magnetic resonance imaging to evaluate autoimmune prostatitis in rats[J]. Chin J Magn Reson Imaging, 2025, 16(2): 100-106. DOI:10.12015/issn.1674-8034.2025.02.016.

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