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• Original Article •
Evaluation of type-H vessels in bone marrow of type 1 diabetic rabbits based on ultra-small superparamagnetic nano-iron oxide enhanced MRI
LEI Haoran  WANG Kejun  LI Liang  LIU Changsheng  ZHA Yunfei 

Cite this article as: LEI H R, WANG K J, LI L, et al. Evaluation of type-H vessels in bone marrow of type 1 diabetic rabbits based on ultra-small superparamagnetic nano-iron oxide enhanced MRI[J]. Chin J Magn Reson Imaging, 2025, 16(2): 107-113. DOI:10.12015/issn.1674-8034.2025.02.017.


[Abstract] Objective To explore the feasibility of quantitative and visual targeting evaluation of type-H vessel structure in bone marrow of rabbits with type 1 diabetes mellitus (T1DM) based on ultra-small superparamagnetic nano-iron oxide (USPIO) enhanced MRI combined with in vitro micro-computed tomography (Micro-CT) microangiography.Materials and Methods T1DM rabbits were constructed by injecting alloxan into the auricular vein in 20 out of 40 Japanese white rabbits (2-3 month-old). The remaining rabbits received equivalent volumes of normal saline via auricular marginal vein injection as controls. After 4 months of successful modeling, samples from metaphyseal and diaphyseal parts of tibia were taken respectively for sorting and identification of bone marrow Type-H vascular endothelial cells, Type-H vascular immunofluorescence detection, USPIO labeling of bone marrow endothelial cells in vitro and in vivo, MRI and Micro-CT microangiography of bone marrow in vitro. The proportion of Type-H vascular endothelial cells, average fluorescence intensity, T2 value of MRI, vessel volume/tissue volume (VV/TV) and vessel number (VN) were measured respectively.Results Compared with the control group, the proportion of type-H vascular endothelial cells, average fluorescence intensity, VV/TV and VN of bone marrow were significantly decreased in T1DM rabbits (P < 0.05), and the T2 value of MRI in bone marrow showed significant differences before and after USPIO injection (P < 0.05). The metaphyseal and diaphysis of T1DM rabbits were respectively compared with the control group. The proportion of type-H vascular endothelial cells, average fluorescence intensity, maximum change of T2 value and fastest time rate, VV/TV and VN in metaphysis were significantly different from those in the diaphysis (P < 0.05).Conclusions USPIO enhanced MRI combined with in vitro Micro-CT microvascular imaging is feasible for quantitative and visual targeting evaluation of bone marrow type-H vascular structure in T1DM rabbits. This study provides imaging evidence for exploring diabetic bone marrow microangiopathy.
[Keywords] type 1 diabetic rabbits；bone marrow microvessels；endothelial cells；ultra-small superparamagnetic nano-iron oxide；magnetic resonance imaging；micro-CT

LEI Haoran   WANG Kejun   LI Liang   LIU Changsheng   ZHA Yunfei*  

Department of Radiology, Renmin Hospital of Wuhan University, Wuhan 430060, China

Corresponding author: ZHA Y F, E-mail: zhayunfei999@126.com

Conflicts of interest   None.

Received  2024-11-25
Accepted  2025-02-10
DOI: 10.12015/issn.1674-8034.2025.02.017
Cite this article as: LEI H R, WANG K J, LI L, et al. Evaluation of type-H vessels in bone marrow of type 1 diabetic rabbits based on ultra-small superparamagnetic nano-iron oxide enhanced MRI[J]. Chin J Magn Reson Imaging, 2025, 16(2): 107-113. DOI:10.12015/issn.1674-8034.2025.02.017.

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