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• Original Article •
A longitudinal study of changes of brain metabolites in vascular cognitive impairment rats using 3.0 T 1H-MRS
HUANG Ming-ming  CAO Xiao-wan  SHEN Gui-quan  LI Xiao-bao  YU Hui 

DOI:10.12015/issn.1674-8034.2017.09.009.


[Abstract] Objective: To explore the application of 3.0 T 1H-MRS in assessment of longitudinal changes of brain metabolites in vascular cognitive impairment (VCI) rats and its potential value in early diagnosis of VCI disease.Materials and Methods: Philips 3.0 T MR scanner combined with animal coil were applied to obtain 1H-MRS data of brain (mainly including the hippocampus) in VCI rats at different time points (2 weeks, 1 month, 3 months and 5 months), Morris Water maze test and pathologic examination (HE and Nissle staining) were also performed to evaluate the cognitive impairment and pathological changes.Results: Compared with controls, at 2 weeks, significantly cognitive impairment and deduction of Glu/tCr ratio (P<0.05)were found. And reduction of NAA/tCr (P<0.05) and increasing of mI/tCr (P<0.05) were detected at 1 month, at 3 months, the increase of mI/tCr were kept, and also GPC/tCr (P<0.05) and tCho/tCr (P<0.05) were raised, at 5 months, all metabolites recovered to normal level besides mI/tCr. But, HE and Nissle stainings showed that cell structural and morphological changes can also exist in the hippocampus.Conclusions: The abnormal metabolites provided by 3.0 T 1H-MRS maybe can reflect the pathological changes in progression of VCI, objectively. Results from this study suggested that reduction of Glu/tCr at the onset and sustained rise of mI/tCr in the progress of VCI, can be helpful for diagnosis of VCI at its early stage.
[Keywords] Cognition disorders；Blood vessels；Hippocampus；Proton magnetic resonance spectroscopy

HUANG Ming-ming Department of Radiology, Affiliated Hospital of Guizhou Medical University, Guiyang 550004, China

CAO Xiao-wan Department of Radiology, Affiliated Hospital of Guizhou Medical University, Guiyang 550004, China

SHEN Gui-quan Department of Radiology, Affiliated Hospital of Guizhou Medical University, Guiyang 550004, China

LI Xiao-bao Department of Radiology, Affiliated Hospital of Guizhou Medical University, Guiyang 550004, China

YU Hui* Department of Radiology, Affiliated Hospital of Guizhou Medical University, Guiyang 550004, China

*Correspondence to: Yu H, E-mail: 331693861@qq.com

Conflicts of interest   None.

ACKNOWLEDGMENTS  This work is supported by grants from the Natural Science Foundation of China No. 8156070059 Science and Technology Department of Guizhou Province No. Qian-ke-he LG 2012-024, TN2014-51 Higher Education Research Center of Guizhou Province No. Qian-jiao-he KY 2016-012
Received  2017-04-14
Accepted  2017-07-20
DOI: 10.12015/issn.1674-8034.2017.09.009
DOI:10.12015/issn.1674-8034.2017.09.009.

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