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• Original Article •
In vivo MR tracking imaging of SPIO-labeled adipose-derived stem cells transplantation in rat models of brain infarction
CHEN Chang-qing  WANG Xiao-yi  CHEN Chen  FANG Jia-sheng  LIAO Wei-hua  LONG Xue-ying 

DOI:10.3969/j.issn.1674-8034.2010.01.013.


[Abstract] Objective: To label adipose-derived stem cells (ADSCs) by complex of superparamagnetic iron oxide (SPIO) with poly-L-Lysine (PLL) and to investigate the distribution and migration of ADSCs in rat model of ischemia brain injury.Materials and Methods: The ligation of middle cerebral artery under microscope was used to cause focal brain infarction in rats. ADSCs labeled with SPIO were grafted stereotactically into the contralateral cerebral hemisphere. All animals were randomly divided into control group (n=12), SPIO-labeled ADSCs group (n=12) and SPIO-unlabeled ADSCs group (n=12). The neurological severity score (NSS) was used to evaluate neurological function recovery of rats at definite time following the transplantation. The transplanted ADSCs were analyzed in recipient rat brains by pathological method. The biodistribution of grafted ADSCs was monitored noninvasively by MR imaging.Results: Significant improvement in NSS was observed in rats receiving ADSCs transplantation compared with control group after 3 weeks (P<0.05). The transplanted ADSCs preferentially engrafted migrated toward the injured brain through callosum. MR imaging showed remarkable hypointense signal in the region of transplantation site, callosum and the rim of infarction especially in T2WI, T2 gradient-recalled echo sequence.Conclusion: Neurological and functional improvement was observed in rats with ADSCs transplantation following ischemia brain injury. In vivo MR tracking of SPIO-PPL complex labeled ADSCs could evaluate the therapeutic effect and visualize the distribution and migration of SPIO-labeled ADSCs following transplantation.
[Keywords] Adipose tissue；Brain infarction；Stem cell transplantation；Superparamagnetic iron oxide；Magnetic resonance imaging

CHEN Chang-qing Department of Radiology, Xiangya Hospital, Central South University, Changsha 410008, China

WANG Xiao-yi* Department of Radiology, Xiangya Hospital, Central South University, Changsha 410008, China

CHEN Chen Department of Pathology, Xiangya Hospital, Central South University, Changsha 410008, China

FANG Jia-sheng Department of Neurosurgery, Xiangya Hospital, Central South University, Changsha 410008, China

LIAO Wei-hua Department of Radiology, Xiangya Hospital, Central South University, Changsha 410008, China

LONG Xue-ying Department of Radiology, Xiangya Hospital, Central South University, Changsha 410008, China

*Correspondence to: Wang XY, E-mail: cjr.wangxiaoyi@vip.163.com

Conflicts of interest   None.

Received  2009-12-18
Accepted  2010-01-11
DOI: 10.3969/j.issn.1674-8034.2010.01.013
DOI:10.3969/j.issn.1674-8034.2010.01.013.

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