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• Technical Article •
Diffusion weighted imaging features after hepatic right portal vein ligation in rats
HUANG Bing-cang  ZHAN Song-hua  GENG Dao-ying  TAN Wen-li  YANG Shuo-hui 

DOI:10.3969/j.issn.1674-8034.2010.01.014.


[Abstract] Objective: To study the diffusion weighted imaging (DWI) characters of hepatic cell focal apoptosis.Materials and Methods: Thirty SD rats about 300g are operated by hepatic right portal vein ligation and divided into 5 groups. T1WI, T2WI, DWI and apparent diffusion coefficient (ADC) image were obtained at 3 hours, 1 day, 3 days, 7 days, 14 days after the operation. After MRI examination, rats were sacrificed and the right hepatic lobes were sampled for pathology, electronic microscopy and TUNEL staining examination.Results: All right hepatic lobe focal apoptosis in 30 rats was certificated by pathology, electronic microscopy and TUNEL staining examination. Occurring from 3 hours after operation, apoptosis cell gradually increased with time. It was high signal at T1-weighted image in each group after operation, low signal at T2-weighted image after 3 hours and high signal in other groups, and slightly high signal at diffusion weighted image after 3 hours, and high signal in other periods. The ADC value of the right lobe was less than the left lobe in each group (P<0.05), the relative ADC value increased in all groups with time. The ADC value, signal-noise ratio (SNR) and sensitivity of DWI decreased with b value increasing, but the specificity increased.Conclusion: There are focal apoptosis in right hepatic lobes of rats after the portal vein ligation. What’s more, its signal in DWI image and the ADC value is unique, so DWI is useful to judge the success of hepatic vessel ligation and detect focal apoptosis area in vivo.
[Keywords] Apoptosis；Magnetic resonance imaging, diffusion；Rat；Hepatic veins

HUANG Bing-cang Department of Radiology, Huashan Hospital, Fudan University, Shanghai 200040, China

ZHAN Song-hua Department of Radiology, Shanghai Traditional Chinese Medical University Affiliated Shuguang Hospital, Shanghai 200021, China

GENG Dao-ying* Department of Radiology, Huashan Hospital, Fudan University, Shanghai 200040, China

TAN Wen-li Department of Radiology, Shanghai Traditional Chinese Medical University Affiliated Shuguang Hospital, Shanghai 200021, China

YANG Shuo-hui Department of Radiology, Shanghai Traditional Chinese Medical University Affiliated Shuguang Hospital, Shanghai 200021, China

*Correspondence to: Geng DY, E-mail: gengdy@163.com

Conflicts of interest   None.

Received  2009-12-11
Accepted  2010-01-15
DOI: 10.3969/j.issn.1674-8034.2010.01.014
DOI:10.3969/j.issn.1674-8034.2010.01.014.

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