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• Original Article •
MRI of prostate stem cell antigen specific MR molecular probe in human prostate cancer at 3.0 T: in vitro experimental study
REN Jing  YANG Yong  WANG Fang  WEI Guang-quan  GE Ya-li  CHANG Ying-juan  YANG An-gang  HUAN Yi 

DOI:10.3969/j.issn.1674-8034.2010.02.012.


[Abstract] Objective: To investigate the feasibility of in vitro MRI of 7F5@GoldMag probe in prostate cancer on a clinical 3.0 T MR system.Materials and Methods: GoldMagTM-CS nanoparticles solution in different concentration was scanned to ascertain the lowest concentration that can be detected by MR system. Prostate cancer cell line PC-3 and hepatoma cell line SMMC-7721 were cultured (hepatoma cell line SMMC-7721 was control group). The two cell lines were cross-matched with 7F5@GoldMag and non-related IgG@GoldMag. Then, the mixture was incubated at 37℃ for 1 hour. T2WI was obtained and the signal intensity was measured in each group. Statistical analysis were performed to assess the statistical differences of signal intensity by using one-way ANOVA with SPSS 11.5 software package as parameters were normally distributed, P<0.05 was considered as statistically significant difference.Results: The T2WI signal intensity of GoldMagTM-CS was significantly lower than that of agarose gel even if the former was diluted by 640 times. 7F5@GoldMag could specifically decrease T2WI signal intensity of PC-3 cells in vitro.Conclusion: 7F5@GoldMag has target-directed enhancement effect in prostate cancer cell lines in vitro by using a clinical 3.0 T MR scanner.
[Keywords] Prostatic neoplasms；Stem cell；Antigen；Antibodies；Magnetic resonance imaging；Molecular probes

REN Jing Department of Radiology, Xijing Hospital, Fourth Military Medical University, Xi’an 710032, China

YANG Yong Department of Radiology, Xijing Hospital, Fourth Military Medical University, Xi’an 710032, China

WANG Fang Department of Immunology, Fourth Military Medical University, Xi’an 710032, China

WEI Guang-quan Department of Radiology, Xijing Hospital, Fourth Military Medical University, Xi’an 710032, China

GE Ya-li Department of Radiology, Xijing Hospital, Fourth Military Medical University, Xi’an 710032, China

CHANG Ying-juan Department of Radiology, Xijing Hospital, Fourth Military Medical University, Xi’an 710032, China

YANG An-gang Department of Immunology, Fourth Military Medical University, Xi’an 710032, China

HUAN Yi* Department of Radiology, Xijing Hospital, Fourth Military Medical University, Xi’an 710032, China

*Correspondence to: Huan Y, E-mail: huanyi3000@163.com

Conflicts of interest   None.

Received  2010-02-03
Accepted  2010-02-20
DOI: 10.3969/j.issn.1674-8034.2010.02.012
DOI:10.3969/j.issn.1674-8034.2010.02.012.

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