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• Original Article •
In vivo semiquantitative evaluation of liver fat content in alcoholic and nonalcoholic fatty liver rat models: comparison between dual-echo T1-weighted imaging and 1H-MR spectroscopy with correlation of histopathology
YANG Ning-jing  SONG Bin  TANG He-han  CHEN Guang-wen 

DOI:10.3969/j.issn.1674-8034.2010.03.010.


[Abstract] Objective: To investigate the values of 1H-MR spectroscopy (1H-MRS) and gradient-echo T1-weighted MR imaging (in-phase and opposed-phase) in quantifying liver fat content (LFC) in rat models of both nonalcoholic fatty liver disease (NAFLD) and alcoholic liver disease (ALD).Materials and Methods: Total 120 healthy Sprague-Dawle male rats were randomly divided into control group, fatty liver group, alcoholic fatty liver group, and mixed models group. At the baseline, 4th, 8th, and 12th week, at least 6 or more rats were selected to receive dual-echo T1W imaging and 1H-MRS of the liver. Fat indexes (FI) for dual-echo T1W imaging and relative lipid content (RLC) of 1H-MRS were measured and compared to pathological grades of fatty liver.Results: Both FI of dual-echo T1W and RLC of 1H-MRS were correlated linearly with the histopathological grading (P<0.01, r=0.54-0.96). RLC (r=0.89-0.96) showed much closer correlation with histopathology than FI (r=0.54-0.85) in all the experimental rat models. For FI, the differences between the normal and mild, mild and moderate steatosis were not significant (P>0.05), while RLC demonstrated significant differences between the normal and each grade of steatosis (P<0.01). For ALD, RLC and FI have the similar values as to fatty liver content (rRLC=0.886, rFI=0.854), whereas RLC was superior to FI for NAFLD (rRLC=0.95-0.96, rFI=0.54-0.65).Conclusion: In vivo 1H-MRS and dual-echo T1W can quantitatively evaluate both NAFLD and ALD in rat models. 1H-MRS is more accurate and reliable for discrimination mild fatty liver and in NAFLD.
[Keywords] Fatty liver；Magnetic resonance spectroscopy；Gradient echo duo-echo technique

YANG Ning-jing Department of Radiology, West China Hospital of Sichuan University, Chengdu 610041, China; Sichuan Cancer Hospital, Chengdu 610041, China

SONG Bin* Department of Radiology, West China Hospital of Sichuan University, Chengdu 610041, China

TANG He-han Department of Radiology, West China Hospital of Sichuan University, Chengdu 610041, China

CHEN Guang-wen Department of Radiology, West China Hospital of Sichuan University, Chengdu 610041, China

*Correspondence to: Song B, E-mail:cjr.songbin@vip.163.com

Conflicts of interest   None.

Received  2010-01-15
Accepted  2010-03-31
DOI: 10.3969/j.issn.1674-8034.2010.03.010
DOI:10.3969/j.issn.1674-8034.2010.03.010.

[1]
Schwenzer NF, Springer F, Schraml C, et al. Non-invasive assessment and quantification of liver steatosis by ultrasound, computed tomography and magnetic resonance. J Hepatol, 2009, 51(3): 433-445.
[2]
van Werven JR, Schreuder TC, Nederveen AJ, et al. Hepatic unsaturated fatty acids in patients with non-alcoholic fatty liver disease assessed by 3.0T MR spectroscopy. Eur J Radiol, 2010 DOI: . [ DOI: ]
[3]
Zhong L, Chen JJ, Chen J, et al. Nonalcoholic fatty liver disease: quantitative assessment of liver fat content by computed tomography, magnetic resonance imaging and proton magnetic resonance spectroscopy. J Dig Dis, 2009, 10(4): 315-320.
[4]
Hines CD, Yu H, Shimakawa A, et al. Quantification of hepatic steatosis with 3-T MR imaging: validation in ob/ob mice. Radiology, 2010, 254(1):119-128.
[5]
Garbow JR, Lin X, Sakata N, et al. In vivo MRS measurement of liver lipid levels in mice. J Lipid Res, 2004, 45(7):1364-1371.
[6]
Saadeh S, Younossi ZM, Remer EM, et al. The utility of radiological imaging in nonalcoholic fatty liver disease. Gastroenterology, 2002, 123(3):745-750.
[7]
Kawamitsu H, Kaji Y, Ohara T, et al. Feasibility of quantitative intrahepatic lipid imaging applied to the magnetic resonance dual gradient echo sequence. Magn Reson Med Sci. 2003, 2(1):47-50.
[8]
Ronald J, Salo S, Dean K, et al．Nonalcoholic Fatty Liver Disease: Rapid Evaluation of Liver Fat Content with In-Phase and Out-phase MR Imaging. Radiology, 2009, 250(1):130-136.
[9]
Qayyum A, Goh JS, Kakar S, et al. Accuracy of liver fat quantification at MR imaging: comparison of out-phase gradient echo and fat-saturated fast spin-echo techniques-initial experience. Radiology, 2005, 237:(2):507-511.
[10]
Xu ZJ, Fan JG, Wang GL, et al. Rat model of nonalcoholic steatohepatitis with fibrosis by a fat-rich diet.World Chinese Journal of Digestology, 2002,10(4):392-396.
[11]
Hatsugai K, Ohkohchi N, Fukumori T, et al. Mechanism of primary graft non-function in a rat model for fatty liver transplantation. Transpl Int, 2000, 13(Suppl 1):S583-589.
[12]
Nan YM, Wang L, Li LX, et al. The progress of nonalcoholic fatty liver models. Journal of HEBEI Medical University. 2007, 28(1):67-69.
[13]
Chinese Society of Hepatology. Diagnostic criteria of alcoholic liver disease.Chinese Journal of Hepatology, 2003, 11(2):72.
[14]
Chinese Society of Hepatology. Diagnostic criteria of nonalcoholic fatty liver disease. Chinese Journal of Hepatology, 2003, 11(2):71.
[15]
Liu ZY, Liang CH, Wang QS, et al. Evaluation of nonalcoholic fatty liver model in rats using 1H magnetic resonance spectroscopy. World Chinese Journal of Digestology, 2008, 16(15):1612-1616.
[16]
Day CP, James OFW. Steatohepatitis: A tale of two "hits"? Gastroenterology, 1998, 114(4):842-845.
[17]
Thomas EL, Hamilton G, Patel N, et al. Hepatic triglyceride content and its relation to body adiposity: a magnetic resonance imaging and proton magnetic resonance spectroscopy study. Gut2005, 54(1):122-127.
[18]
Zhang HJ, Zhuang H, Liu XE. The epidemiological progress of fatty liver. Chinese Journal of Epidemiology, 2004, 25(7):630-631.
[19]
Machann J, Thamer C, Schnoedt B, et al. Hepatic lipid accumulation in healthy subjects: a comparative study using spectral fat-selective MRI and volume-localized 1H-MR spectroscopy. Magn Reson Med, 2006, 55(4):913-917.
[20]
Middleton MS, Sirlin CB, Pinto N, et al. Simultaneous T2 relaxation time and fat fraction measurement in children with nonalcoholic fatty liver disease or nonalcoholic steatohepatitis using breath-hold MR spectroscopic techniques. In: Radiological Society of North America scientific assembly and annual meeting program. Oak Brook, Ill: Radiological Society of North America, 2006: 87.
[21]
Chan DC, Watts GF, Ng TW, et al. Measurement of liver fat by magnetic resonance imaging: Relationships with body fat distribution, insulin sensitivity and plasma lipids in healthy men. Diabetes Obes Metab, 2006,8(6):698-702.
[22]
Fan MX, Tian JM, Lu JP, et al. Preliminary evaluation of fatty liver in rats by in vivo 1H MR spectroscopy.Chin J Med Imaging Technol,2004, 20(10):1503-1505.

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