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• Original Article •
Ferritin coordinates with SPIO in tracking C6 rat glioma cells by MRI
WANG Jian-dong  HU Qiu-ju  ZHU Fei-peng  CHANG Shuang-hui  ZHANG Fan  ZHOU Xiao-jun  LU Guang-ming 

DOI:10.3969/j.issn.1674-8034.2010.04.013.


[Abstract] Objective: To investigate the effect of ferritin protein overexpression on superparamagnetic iron oxide (SPIO) particle labeling of C6 rat glioma cells, and to track the labeled cells in vivo using magnetic resonance imaging (MRI).Materials and Methods: Plasmid of H-chain of murine ferritin gene was constructed and transfected into C6 cells. The parental and transfected C6 cells labeled with SPIO were bilaterally inoculated subcutaneously into nude mice. The mice were subjected to multiple T2-weighted MR scans after C6 cell inoculation. The mice were sacrificed in two weeks and the concentration of iron in the tumor tissue was measured.Results: The concentration of iron in xenografts derived from ferritin plasmid transfected and SPIO labeled C6 cells was significant higher than that in xenografts from parental C6 cells labeled with SPIO (P=0.034, n=5). Ferritin transfected C6 cells with SPIO labeling showed an improved T2 contrast in vivo after inoculating of 2 days (P=0.012, n=5), 6 days (P=0.003, n=5), and 13 days (P=0.021, n=5).Conclusion: Coordinating ferritin with SPIO can lead to a longer MRI cellular tracking period.
[Keywords] Ferritin reporter；Superparamagnetic iron oxide；Cell tracking；Magnetic resonance imaging

WANG Jian-dong Department of Medical Radiology, Nanjing General Hospital, PLA, Nanjing 210002, China; Department of Pathology, Nanjing General Hospital, PLA, Nanjing 210002, China

HU Qiu-ju Department of Medical Radiology, Nanjing General Hospital, PLA, Nanjing 210002, China

ZHU Fei-peng Department of Medical Radiology, Nanjing General Hospital, PLA, Nanjing 210002, China

CHANG Shuang-hui Department of Medical Radiology, Nanjing General Hospital, PLA, Nanjing 210002, China

ZHANG Fan Department of Medical Radiology, Nanjing General Hospital, PLA, Nanjing 210002, China

ZHOU Xiao-jun Department of Pathology, Nanjing General Hospital, PLA, Nanjing 210002, China

LU Guang-ming* Department of Medical Radiology, Nanjing General Hospital, PLA, Nanjing 210002, China

*Correspondence to: Lu GM, E-mail: cjr.luguangming@vip.163.com

Conflicts of interest   None.

Received  2010-02-08
Accepted  2010-04-12
DOI: 10.3969/j.issn.1674-8034.2010.04.013
DOI:10.3969/j.issn.1674-8034.2010.04.013.

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