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Review
Applications of cellular MRI and cell labeling contrast agents in cell transplantation
XU Bo  LIANG Bi-ling  DENG Yu-bin 

DOI:10.3969/j.issn.1674-8034.2011.03.010.


[Abstract] The clinical applications of cell transplantation increase gradually along with the developments of preclinical researches. However, it is necessary to track the fate of transplanted cells in both clinical applications and preclinical researches. Magnetic resonance imaging is non-invasive and time continuous, and is an ideal method to continuous track transplanted cells in vivo. By labeling the cells with MRI contrast agents, we can monitor the migration, distribution and functional status of transplanted cells with MRI. Here we reviewed the progresses of magnetic resonance imaging and cell-labeling contrast agents in cell transplantation.
[Keywords] Magnetic resonance imaging;Contrast agent;Cell labeling;Cell transplantation

XU Bo Institute of Pathophysiology, SUN Yat-sen University, Guangzhou 510080, China

LIANG Bi-ling Department of Radiology; Sun Yat-sen Memorial Hospital, Guangzhou 510120, China

DENG Yu-bin Institute of Pathophysiology, SUN Yat-sen University, Guangzhou 510080, China

*Correspondence to: Deng YB, E-mail: dengyub@mail.sysu.edu.cn

Conflicts of interest   None.

Received  2010-11-01
Accepted  2011-01-06
DOI: 10.3969/j.issn.1674-8034.2011.03.010
DOI:10.3969/j.issn.1674-8034.2011.03.010.

[1]
Romano G. Stem cell transplantation therapy: controversy over ethical issues and clinical relevance. Drug News Perspect, 2004, 17(10):637-645.
[2]
Modo M. Noninvasive imaging of transplanted cells. Curr Opin Organ Transplant, 2008, 13(6):654-658.
[3]
Baligand C,Vauchez K, Fiszman M, et al. Discrepancies between thefate ofmyoblast xenograft in mouse leg muscle and NMR label per2sistency after loadingwith Gd2DTPA or SPIOs. Gene Ther, 2009, 16(6):734-745.
[4]
Crich SG, Biancone L, Cantaluppi V, et al. Improved Route for the Visualization of Stem Cells Labeled With a Gd-/Eu-Chelate as Dual (MRI and Fluorescence) Agent. Magnetic Resonance in Medicine, 2004, 51(5):938-944.
[5]
Terreno E, Crich SG, Belfiore S, et al. Effect of the Intracellular Localization of a Gd-Based Imaging Probe on the Relaxation Enhancement of Water Protons. Magnetic Resonance in Medicine, 2006, 55(3):491-497
[6]
邓宇斌,毕小彬,沈君,等. Gd-DTPA标记骨髓间质干细胞在脊髓损伤模型中的磁共振示踪研究.中国医学影像技术, 2008, 24(6):843-847.
[7]
Gonzalez-Lara Laura E., Xiaoyun Xu, Klara Hofstetrova, et al. The Use of Cellular Magnetic Resonance Imaging to Track the Fate of Iron-Labeled Multipotent Stromal Cells after Direct Transplantation in a Mouse Model of Spinal Cord Injury. Mol Imaging Biol, 2010 DOI:
[8]
Bonnemain B. Superparamagnetic agents in magnetic resonance imaging: physicochemical characteristics and clinical applications. J Drug Targeting, 1998, 6(3):167-174.
[9]
陈长青,王小宜,陈晨,等. SPIO标记脂肪干细胞移植治疗大鼠脑梗死的磁共振示踪成像研究.磁共振成像, 2010,1(1): 50-54.
[10]
Frank JA, Anderson SA, Kalsih H, et al. Methods for magnetically labeling stem and other cells for detection by in vivo magnetic resonance imaging. Cytotherapy, 2004, 6(6): 621-625.
[11]
Bulte JW, Kraitchman DL. Iron oxide MR contrast agents for molecular and cellular imaging. NMR Biomed, 2004, 17(7):484-499.
[12]
王建东,胡秋菊,朱飞鹏,等.铁蛋白与SPIO在磁共振细胞活体示踪中的增效作用.磁共振成像, 2010, 1(4): 299-304.
[13]
RogersWJ, Meyer CH, Kramer CM, et al. Technology insight: in vivo cell tracking by use of MRI. Nat Clin Pract Cardiovasc Med, 2006, 3(10):554-562.
[14]
Ahrens ET, Flores R, Xu HY, et al. In vivo imaging platform for tracking immunotherapeutic cells. Nat Biotechnol, 2005, 23(8):983-987.
[15]
Janjic JM, Ahrens ET. Fluorine-containing nanoemulsions for MRI cell tracking. Wiley Interdiscip Rev Nanomed Nanobiotechnol, 2009, 1(5):492-501.
[16]
Rausch M, Baumann D, Neubacher U, et al. In-vivo visualization of phagocytotic cells in rat brains after transient ischemia by USPIO. NMR Biomed, 2002, 15(4):278-283.
[17]
Saleh A, Wiedermann D, Schroeter M, et al. Central nervous system inflammatory response after cerebral infarction as detected by magnetic resonance imaging. NMR Biomed, 2004, 17(4):163-169.
[18]
de Vries IJM, Lesterhuis WJ, Barentsz JO, et al. Magnetic resonance tracking of dendritic cells in melanoma patients for monitoring of cellular therapy. Nat Biotechnol, 2005, 23(11):1407-1413.
[19]
Himmelreich U, Aime S, Hieronymus T, et al. A responsive MRI contrast agent to monitor functional cell status. Neuroimage, 2006, 32(3):1142-1149.
[20]
Jendelová P, Herynek V, DeCroos J, et al. Imaging the fate of implanted bone marrow stromal cells labeled with superparamagnetic nanoparticles. Magn Reson Med, 2003, 50(4):767-776.
[21]
Pawelczyk E, Jordan EK, Balakumaran A, et al. In vivo transfer of intracellular labels from locally implanted bone marrow stromal cells to resident tissue macrophages. PLoS One, 2009, 4(8):e6712.
[22]
Arbab AS, Yocum GT, Kalsih H, et al. Efficient magnetic cell labeling with protamine sulfate complexed to ferumoxides for cellular MRI. Blood, 2004, 104(4):1217-1223.
[23]
van den Bos EJ, Wagner A, Mahrholdt H, et al. Improved efficacy of stem cell labeling for magnetic resonance imaging studies by the use of cationic liposomes. Cell Transplant, 2003, 12(7):743-756.
[24]
Moore A, Basilion JP, Chiocca EA, et al. Measuring transferrin receptor gene expression by NMR imaging. Biochim Biophys Acta, 1998, 1402(3):239-249.
[25]
任静,杨勇,王芳,等.前列腺干细胞抗原特异性MR分子探针体外成像实验研究.磁共振成像, 2010, 1(2): 138-141.
[26]
Geninatti CS, Lanzardo S, Barge A, et al. Visualization throughmagnetic resonance imaging of DNA internalized following in vivoelectroporation. Mol Imaging, 2005, 4(1):7-17.
[27]
Walczak P, Kedziorek DA, Gilad AA, et al. Instant MR labeling of stem cells using magnetoelectroporation. Magn Reson Med, 2005, 54(4):769-774.

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