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• Original Article •
Metabolic changes of liver cell apoptosis: an in vivo 1H magnetic resonance spectroscopy study
YOU Ke-zeng  SHEN Zhi-wei  CAO Zhen  YANG Zhong-xian  CHENG Xiao-fang  YAO Jian-li  WU Ren-hua 

DOI:10.3969/j.issn.1674-8034.2011.04.010.


[Abstract] Objective: To explore the feasibility of dectecting liver cell apoptosis in liver injury model by using magnetic resonance spectroscopy (MRS), and endeavor to find the characteristic changes of MRS.Materials and Methods: Twenty-seven New Zealand white rabbits were randomly divided into the control group (n=10) and the treated group (n=17). The rabbits in the treated group were intraperitoneally (i.p.) injected with sodium selenite while the ones in the control group with saline. At 48 hours after the injection, in vivo liver 1H-MRS were collected using point resolved spectroscopy sequence under the same conditions. After MR scanning, the rabbits were sacrificed immediately for histopathological examination. The 1H-MRS data were processed by SAGE 7.0 software to analyze the ratio of lipid/water (Lip/Water) and total choline/water (tCho/Water) in unsuppressed water spectral and the ratio of total choline/lipid (tCho/Lip) in suppressed water spectra. Statistical analysis was done by a two-tailed independent t test.Results: All of the listed tests were fully finished in 25 rabbits (15 treated and 10 controls). The MRS curves, mainly with peaks appearing at correct positions, could be analyzed. From MR spectra, there are some differences between the treated group and the control one. In unsuppressed water spectra, the ratio of Lip/Water (0.029±0.024, n=12) in treated group is higher than that in control group (0.014±0.007, n=10) (P<0.05); the ratio of tCho/Water (0.001±0.0005, n=12) in the treated group is significantly lower than that (0.0030±0.0003) in control group (P<0.05). In suppressed water spectra, the ratio of tCho/Lip (0.093±0.007, n=12) in the treated group is also significantly lower than that in control group (0.210±0.061, n=10) (P<0.05). The percentage of apoptotic cells was 9.2±3.1% in the model rats, and 0.27±0.07% in controls (P<0.001).Conclusion: After the injections, there are some characteristic differences between the 1H-MRS in the treatment group and that in the control group. In vivo 1.5 T 1H-MRS can be used to detect metabolic activity of this selenite-induced rabbit liver injury model, and liver cell apoptosis as well.
[Keywords] Cell apoptosis；Magnetic resonance spectroscopy；Liver

YOU Ke-zeng Department of Radiology, the Second Affiliated Hospital of Shantou University Medical College, Shantou 515041, China

SHEN Zhi-wei Department of Radiology, the Second Affiliated Hospital of Shantou University Medical College, Shantou 515041, China

CAO Zhen Department of Radiology, the Second Affiliated Hospital of Shantou University Medical College, Shantou 515041, China

YANG Zhong-xian Department of Radiology, the Second Affiliated Hospital of Shantou University Medical College, Shantou 515041, China

CHENG Xiao-fang Department of Radiology, the Second Affiliated Hospital of Shantou University Medical College, Shantou 515041, China

YAO Jian-li Department of Radiology, the Second Affiliated Hospital of Shantou University Medical College, Shantou 515041, China

WU Ren-hua* Department of Radiology, the Second Affiliated Hospital of Shantou University Medical College, Shantou 515041, China

*Correspondence to: Wu RH, E-mail: cjr.wurenhua@vip.163.com

Conflicts of interest   None.

Received  2011-03-01
Accepted  2011-05-11
DOI: 10.3969/j.issn.1674-8034.2011.04.010
DOI:10.3969/j.issn.1674-8034.2011.04.010.

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