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• Overseas Papers •
Evaluation of CLT1-(Gd-DTPA) for MR molecular imaging in a mouse breast cancer model
Ye Furong  Jeong Eun-Kee  Parker Denis  Lu Zheng-Rong 

DOI:10.3969/j.issn.1674-8034.2011.05.002.


[Abstract] A peptide targeted contrast agent, CLT1- (Gd-DTPA), was investigated for molecular imaging of fibrin-fribronectin complexes in tumor stroma with magnetic resonance imaging (MRI). The contrast agent was evaluated in female nude mice bearing MDA-MB-231 human breast carcinoma xenografts on a Siemens 3T clinical scanner with a clinical agent Gd (DTPA-BMA) as a non-targeted control. CLT1- (Gd-DTPA) specifically bound to tumor tissue and resulted in significant tumor contrast enhancement at a dose of 0.05 mmol/kg for at least 60 minutes after injection. In contrast, a non-targeted contrast agent, Gd(DTPA-BMA), cleared rapidly from the body with little tumor enhancement after 30 minutes post-injection at a dose of 0.1 mmol/kg. CLT1- (Gd-DTPA) had little non-specific binding in blood and normal tissues, including the liver and muscle, resulting in comparable non-specific enhancement in normal tissues to the control agent. The study has shown that CLT1-(Gd-DTPA) can bind to the tumor tissue, resulting in significant tumor enhancement in a mouse breast cancer model. The targeted contrast agent has a potential for MR molecular imaging of breast cancer.
[Keywords] Magnetic resonance imaging；Molecular imaging；Targeted contrast agent；Mouse；Breast cancer

Ye Furong Department of Pharmaceutics and Pharmaceutical Chemistry, University of Utah, Salt Lake City, Utah, USA

Jeong Eun-Kee Department of Radiology, University of Utah, Salt Lake City, Utah, USA

Parker Denis Department of Radiology, University of Utah, Salt Lake City, Utah, USA

Lu Zheng-Rong* Department of Biomedical Engineering, Case Western Reserve University, Cleveland, OH 44106, USA

*Correspondence to: Dr. Zheng-Rong Lu, E-mail: zxl125@case.edu

Conflicts of interest   None.

Received  2011-07-11
Accepted  2011-08-19
DOI: 10.3969/j.issn.1674-8034.2011.05.002
DOI:10.3969/j.issn.1674-8034.2011.05.002.

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