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• Original Article •
OxLDL-targeted iron oxide particles for magnetic resonance detection of carotid atherosclerotic lesions in vivo
FU Ming-cui  WEN Song  LU Tong  LIU Dong-fang  ZHOU Guan-hui  AN Yan-li  LIAO Lei  JU Sheng-hong  TENG Gao-jun 

DOI:10.3969/j.issn.1674-8034.2011.05.003.


[Abstract] Objective: The aim of this study was to determine the role of oxLDL targeted iron oxide particles in atherosclerotic lesions imaging in carotid atherosclerosis lesions of apoE-/- mice.Materials and Methods: PEG coated USPIO was conjugated with anti-mouse-oxLDL antibodies to construct an anti-oxLDL-USPIO probe while IgG-USPIO and unconjugated USPIO was used as controls. All formulations were characterized by using TEM, DLS and ELISA. The model of perivascular collar in the left carotid in apoE-/- mice was used. The MRI was processed in pre-, 8 h and 24 h after tain vein injection of a 30 mg Fe/Kg dose of USPIO nanoparticles.Results: MRI of atherosclerotic lesion, as manifested by signal loss, was observed after administration of targeted USPIO at 8 h and 24 h, the percent of signal reduced was-30.4±16% and-34.7±19% while the IgG-USPIO, pure USPIO and inhibition group have limited signal reduction, the difference was significant, P<0.05. Immunohistochemistry confirmed the presence of oxLDL/CD68 and iron particles.Conclusion: This study demonstrates that anti-oxLDL-USPIO can be used in imaging atherosclerotic lesions within 24 hours. It suggests a clinically translatable platform for the detection of oxLDL in atherosclerotic plaque in vivo and will play a role in therapeutic evaluation of atherosclerotic carotid plaque.
[Keywords] Atherosclerosis；Antibody；oxLDL；Magnetic resonance imaging

FU Ming-cui Department of Radiology, Zhong-Da Hospital, Southeast University, Nanjing 210009, China

WEN Song Department of Radiology, Zhong-Da Hospital, Southeast University, Nanjing 210009, China

LU Tong Department of Radiology, Zhong-Da Hospital, Southeast University, Nanjing 210009, China

LIU Dong-fang Department of Radiology, Zhong-Da Hospital, Southeast University, Nanjing 210009, China

ZHOU Guan-hui Department of Radiology, Zhong-Da Hospital, Southeast University, Nanjing 210009, China

AN Yan-li Department of Radiology, Zhong-Da Hospital, Southeast University, Nanjing 210009, China

LIAO Lei Department of Radiology, Zhong-Da Hospital, Southeast University, Nanjing 210009, China

JU Sheng-hong Department of Radiology, Zhong-Da Hospital, Southeast University, Nanjing 210009, China

TENG Gao-jun* Department of Radiology, Zhong-Da Hospital, Southeast University, Nanjing 210009, China

*Correspondence to: Teng GJ, E-mail: gjteng@vip.sina.com

Conflicts of interest   None.

Received  2011-07-05
Accepted  2011-08-17
DOI: 10.3969/j.issn.1674-8034.2011.05.003
DOI:10.3969/j.issn.1674-8034.2011.05.003.

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