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• Original Article •
The study of both MR diffusion-weighted imaging and expression of VEGF compared with pathology in dog model of liver fibrosis
ZHANG Ya-qin  LI Cong-rui  HU Yue-qun  LUO Lei  LIAO Yun-jie  WANG Wei 

DOI:10.3969/j.issn.1674-8034.2011.05.005.


[Abstract] Objective: To evaluate the change of diffusion-weighted MR imaging markers and expression of VEGF in dog model of liver fibrosis, and compared with histopathological features to investigate the diagnostic methods for liver fibrosis.Materials and Methods: 50% of the carbon tetrachloride (CCL4) oil solution were injected in peritoneal cavity in dogs, and were received the high fat diet. The dogs were underwent MR examination and liver biopsy every 4 weeks. The liver tissue were underwent pathology routine HE staining, Masson staining and immunohistochemistry VEGF staining, divided into groups by the results of pathological stage, the level of VEGF expression, the corresponding ADC values and eADC values were compared among different stages of liver fibrosis, analyzing the correlations among them.Results: Grouping according to different stage of fibrosis, S0 (n=12), S1 (n=9), S2 (n=9), S3 (n=10), S4 (n=10). ADC values decreasing and eADC values increasing as the stage of fibrosis progressed (P<0.05). There was a good relationship between ADC (eADC) and stage of fibrosis. The expression of VEGF increased with increasing degrees of liver fibrosis.Conclusions: An intraperitoneal injection of carbon tetrachloride in oil solution with the high fat diet in dogs can be established different stages of liver fibrosis. The value of ADC (eADC) can quantitatly analysis deferent degree hepatic fibrosis, they are more meaningful evaluation indicators to evaluate the hepatic fibrosis. VEGF may play an important role in the process of liver fibrosis in chronic liver disease.
[Keywords] Liver fibrosis；Diffusion-weighted MR imaging；Vascular endothelial growth factor；Animal model

ZHANG Ya-qin Department of Radiology, the Third Xiangya Hospital of Central South University, Changsha 410013, China

LI Cong-rui Department of Radiology, the Third Xiangya Hospital of Central South University, Changsha 410013, China

HU Yue-qun Department of Radiology, the Third Xiangya Hospital of Central South University, Changsha 410013, China

LUO Lei Department of Radiology, the Third Xiangya Hospital of Central South University, Changsha 410013, China

LIAO Yun-jie Department of Radiology, the Third Xiangya Hospital of Central South University, Changsha 410013, China

WANG Wei* Department of Radiology, the Third Xiangya Hospital of Central South University, Changsha 410013, China

*Correspondence to: Wang W, E-mail: wawe01cn@yahoo.com.cn

Conflicts of interest   None.

Received  2011-06-22
Accepted  2011-08-20
DOI: 10.3969/j.issn.1674-8034.2011.05.005
DOI:10.3969/j.issn.1674-8034.2011.05.005.

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