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• Original Article •
Liver iron quantification by 3.0 T MRI: calibration on a rabbit model
LONG Li-ling  PENG Peng  HUANG Zhong-kui  LI Chun-yan  ZHAO Fan-yu  LONG Mei  LI Wen-mei 

DOI:10.3969/j.issn.1674-8034.2012.06.010.


[Abstract] Objective: To explore the feasibility of liver iron quantification at 3.0 T MRI.Materials and Methods: Forty-two rabbits underwent iron dextran loading (15 mg/kg/week) from 1—15 weeks. 2 controls were studied as well. MRI signal intensity ratio (SIR) was measured using a gradient-echo sequence, and T2 (R2=1/T2) measured using an 8-echo spin-echo sequence at 3.0 T. Exvivo hepatic pathology was obtained for all rabbits. Postmortem assessments of liver iron concentration (LIC) were conducted in an atomic absorption spectrophotometer. MRI measures were fitted against LIC using linear regression for the first 30 rabbits. The remaining 12 were used to test the accuracy of the derived model.Results: Hepatic pathology confirmed that liver iron overload rose with administered amount over time. Experimental group’s liver T2 values, SIR, and LIC ranged from 0.3—1.5 ms, 10.2—48.3, 1.3—9.1 mg/g dry tissure, respectively. Median was 1.0 ms, 18.9, 4.6 mg/g dry tissure, respectively. LIC was linearly correlated to both R2 (r = 0.948, P=0.000) and SIR (r=-0.845, P=0.000). Through the linear regression procedure, a slope of 96.426,-5.924 and an intercept of -0.920, 10.581 were found respectively for R2-LIC and SIR-LIC. In the 12 test rabbits, the predicted LICs using the equations agreed well with the results obtained using spectrophotometer.Conclusion: MRI quantification of liver iron overload is feasible at 3.0 T within a certain LIC limits.
[Keywords] Liver；Iron overload；Magnetic resonance imaging；Animals, laboratory

LONG Li-ling * Department of Radiology, the First Affiliated Hospital of Guangxi Medical University, Nanning 530021, China

PENG Peng Department of Radiology, the First Affiliated Hospital of Guangxi Medical University, Nanning 530021, China

HUANG Zhong-kui Department of Radiology, the First Affiliated Hospital of Guangxi Medical University, Nanning 530021, China

LI Chun-yan Department of Radiology, the First Affiliated Hospital of Guangxi Medical University, Nanning 530021, China

ZHAO Fan-yu Department of Radiology, the First Affiliated Hospital of Guangxi Medical University, Nanning 530021, China

LONG Mei Department of Radiology, the First Affiliated Hospital of Guangxi Medical University, Nanning 530021, China

LI Wen-mei Department of Radiology, the First Affiliated Hospital of Guangxi Medical University, Nanning 530021, China

*Correspondence to: Long LL, E-mail: Cjr.longliling@vip.163.com

Conflicts of interest   None.

Received  2012-09-20
Accepted  2012-10-27
DOI: 10.3969/j.issn.1674-8034.2012.06.010
DOI:10.3969/j.issn.1674-8034.2012.06.010.

[1]
陆洪波，沈寅琛，王靖. 地中海贫血筛查诊断咨询的探讨. 中国优生与遗传杂志, 2009, 17(12): 115-116.
[2]
Vermylen C. What is new in iron overload? Eur J Pediatr, 2008, 167(4): 377-381.
[3]
Angelucci E, Brittenham GM, McLaren CE, et al. Hepatic iron concentration and total body iron stores in thalassemia major. N Engl J Med, 2000, 343(5): 327-331.
[4]
Stark DD, Bass NM, Moss AA, et al. Nuclear magnetic resonance imaging of experimentally induced liver disease. Radiology, 1983, 148(3): 743-751.
[5]
Alexopoulou E, Stripeli F, Baras P, et al. R2 relaxometry with MRI for the quantification of tissue iron overload in β-thalassemic patients. J Magn Reson Imaging, 2006, 23(2): 163-170.
[6]
Alústiza JM, Artetxe J, Castiella A, et al. MR quantification of hepatic iron concentration. Radiology, 2004, 230(2): 479-484.
[7]
St Pierre TG, Clark PR, Chua-anusorn W, et al. Noninvasive measurement and imaging of liver iron concentrations using proton magnetic resonance. Blood, 2005, 105(2): 855-861.
[8]
Gandon Y, Olivié D, Guyader D, et al. Non-invasive assessment of hepatic iron stores by MRI. Lancet, 2004, 363(9406): 357-362.
[9]
Storey P, Thompson AA, Carqueville CL, et al. R2* imaging of transfusional iron burden at 3 T and comparison with 1.5 T. J Magn Reson Imaging, 2007, 25(3): 540-547.
[10]
Guo H, Au WY, Cheung JS, et al. Myocardial T2 quantitation in patients with iron overload at 3 Tesla. J Magn Reson Imaging, 2009, 30(2): 394-400.
[11]
Meloni A, Positano V, Keilberg P, et al. Feasibility, reproducibility, and reliability for the T2* iron evaluation at 3 T in comparison with 1.5 T. Magn Reson Med, 2012, 68(2): 543-551.
[12]
Wood JC, Otto-Duessel M, Aguilar M, et al. Cardiac iron determines cardiac T2*, T2, and T1 in the gerbil model of iron cardiomyopathy. Circulation, 2005, 112(4): 535-543.
[13]
Wood JC, Aguilar M, Otto-Duessel M, et al. Influence of iron chelation on R1 and R2 calibration curves in gerbil liver and heart. Magn Reson Med, 2008, 60(1): 82-89.
[14]
Halliday JW, Searle J. Hepatic iron deposition in human disease and animal models. Biometals, 1996, 9(2): 205-209.
[15]
Obejero-Paz CA, Yang T, Dong WQ, et al. Deferoxamine promotes survival and prevents electrocardiographic abnormalities in the gerbil model of iron-overload cardiomyopathy. J Lab Clin Med, 2003, 141(2): 121-130.
[16]
Papakonstandinou OG, Maris TG, Kostadirou V, et al. Assessment of iron overload by T2-quantitative magnetic resonance imaging: correlation of T2-QMRI measurement with serum ferritin concentration and histologic grading of siderosis. Magn Reson Imaging, 1995, 13(7): 967-977.
[17]
Wood JC, Ghugre N. Magnetic resonance imaging assessment of excess iron in thalassmia, sickle cell disease and others iron overload diseases. Hemoglobin, 2008, 32(1-2): 85-96.
[18]
Olivieri NF, Brittenham GM. Iron-chelating therapy and the treatment of thalassemia. Blood, 1997, 89(3): 739-761.

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