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• Original Article •
Liver iron quantification by 3.0 T MRI: calibration on a rabbit model
LONG Li-ling  PENG Peng  HUANG Zhong-kui  LI Chun-yan  ZHAO Fan-yu  LONG Mei  LI Wen-mei 

DOI:10.3969/j.issn.1674-8034.2012.06.010.


[Abstract] Objective: To explore the feasibility of liver iron quantification at 3.0 T MRI.Materials and Methods: Forty-two rabbits underwent iron dextran loading (15 mg/kg/week) from 1—15 weeks. 2 controls were studied as well. MRI signal intensity ratio (SIR) was measured using a gradient-echo sequence, and T2 (R2=1/T2) measured using an 8-echo spin-echo sequence at 3.0 T. Exvivo hepatic pathology was obtained for all rabbits. Postmortem assessments of liver iron concentration (LIC) were conducted in an atomic absorption spectrophotometer. MRI measures were fitted against LIC using linear regression for the first 30 rabbits. The remaining 12 were used to test the accuracy of the derived model.Results: Hepatic pathology confirmed that liver iron overload rose with administered amount over time. Experimental group’s liver T2 values, SIR, and LIC ranged from 0.3—1.5 ms, 10.2—48.3, 1.3—9.1 mg/g dry tissure, respectively. Median was 1.0 ms, 18.9, 4.6 mg/g dry tissure, respectively. LIC was linearly correlated to both R2 (r = 0.948, P=0.000) and SIR (r=-0.845, P=0.000). Through the linear regression procedure, a slope of 96.426,-5.924 and an intercept of -0.920, 10.581 were found respectively for R2-LIC and SIR-LIC. In the 12 test rabbits, the predicted LICs using the equations agreed well with the results obtained using spectrophotometer.Conclusion: MRI quantification of liver iron overload is feasible at 3.0 T within a certain LIC limits.
[Keywords] Liver；Iron overload；Magnetic resonance imaging；Animals, laboratory

LONG Li-ling * Department of Radiology, the First Affiliated Hospital of Guangxi Medical University, Nanning 530021, China

PENG Peng Department of Radiology, the First Affiliated Hospital of Guangxi Medical University, Nanning 530021, China

HUANG Zhong-kui Department of Radiology, the First Affiliated Hospital of Guangxi Medical University, Nanning 530021, China

LI Chun-yan Department of Radiology, the First Affiliated Hospital of Guangxi Medical University, Nanning 530021, China

ZHAO Fan-yu Department of Radiology, the First Affiliated Hospital of Guangxi Medical University, Nanning 530021, China

LONG Mei Department of Radiology, the First Affiliated Hospital of Guangxi Medical University, Nanning 530021, China

LI Wen-mei Department of Radiology, the First Affiliated Hospital of Guangxi Medical University, Nanning 530021, China

*Correspondence to: Long LL, E-mail: Cjr.longliling@vip.163.com

Conflicts of interest   None.

Received  2012-09-20
Accepted  2012-10-27
DOI: 10.3969/j.issn.1674-8034.2012.06.010
DOI:10.3969/j.issn.1674-8034.2012.06.010.

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