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• Original Article •
Study of Gd-DTPA labeled bone marrow mesenchymal stem cells in vitro with 7.0 T MRI
GENG Kuan  JIA Yan-long  HUANG De-xiao  YI Mei-zhi  YANG Zhong-xian  LIN Yan  WU Ren-hua 

DOI:10.3969/j.issn.1674-8034.2013.06.007.


[Abstract] Objective: The aim of this study was to explore the possibility of the rat bone mesenchymal stem cells (BMSCs) labeled with standard contrast agents (Gd-DTPA) for stem cell tracking.Materials and Methods: BMSCs from bilateral femur of rats were cultured and propagated. The BMSCs uptaked Gd-DTPA. Electron microscopy was performed to detect the distribution of Gd-DTPA particles in BMSCs. Viability and proliferation of labeled BMSCs were evaluated using trypan blue exclusion assays and MTT assay. Labeled BMSCs were detected with T1-weighed MR imaging in vitro.Results: The presence of Gd-DTPA particles inside the BMSCs was definitely detected by transmission electron microscopy. There was no difference in viability and proliferation between the labeled and unlabeled cells. The labeled BMSCs demonstrated the high signal intensity on T1-weighted MRI in vitro.Conclusion: Gd-DTPA shows no evident adverse effect on the function of labeled BMSCs. Gd-DTPA can be used for the MR imaging tracking of labeled BMSCs.
[Keywords] Mesenchymal stem cells；Magnetic resonance imaging；Staining and labeling；Gadolinium DTPA；Animals, laboratory

GENG Kuan Medical Imaging Center, the 2nd Affiliated Hospital, Medical College of Shantou University, Shantou 515041, China

JIA Yan-long Medical Imaging Center, the 2nd Affiliated Hospital, Medical College of Shantou University, Shantou 515041, China

HUANG De-xiao Medical Imaging Center, the 2nd Affiliated Hospital, Medical College of Shantou University, Shantou 515041, China

YI Mei-zhi Medical Imaging Center, the 2nd Affiliated Hospital, Medical College of Shantou University, Shantou 515041, China

YANG Zhong-xian Medical Imaging Center, the 2nd Affiliated Hospital, Medical College of Shantou University, Shantou 515041, China

LIN Yan Medical Imaging Center, the 2nd Affiliated Hospital, Medical College of Shantou University, Shantou 515041, China

WU Ren-hua * Medical Imaging Center, the 2nd Affiliated Hospital, Medical College of Shantou University, Shantou 515041, China

*Correspondence to: Wu RH, Email: rhwu@stu.edu.cn

Conflicts of interest   None.

Received  2013-08-22
Accepted  2013-10-10
DOI: 10.3969/j.issn.1674-8034.2013.06.007
DOI:10.3969/j.issn.1674-8034.2013.06.007.

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