• Original Article •
Study of Gd-DTPA labeled bone marrow mesenchymal stem cells in vitro with 7.0 T MRI
DOI:10.3969/j.issn.1674-8034.2013.06.007.
| [Abstract] Objective: The aim of this study was to explore the possibility of the rat bone mesenchymal stem cells (BMSCs) labeled with standard contrast agents (Gd-DTPA) for stem cell tracking.Materials and Methods: BMSCs from bilateral femur of rats were cultured and propagated. The BMSCs uptaked Gd-DTPA. Electron microscopy was performed to detect the distribution of Gd-DTPA particles in BMSCs. Viability and proliferation of labeled BMSCs were evaluated using trypan blue exclusion assays and MTT assay. Labeled BMSCs were detected with T1-weighed MR imaging in vitro.Results: The presence of Gd-DTPA particles inside the BMSCs was definitely detected by transmission electron microscopy. There was no difference in viability and proliferation between the labeled and unlabeled cells. The labeled BMSCs demonstrated the high signal intensity on T1-weighted MRI in vitro.Conclusion: Gd-DTPA shows no evident adverse effect on the function of labeled BMSCs. Gd-DTPA can be used for the MR imaging tracking of labeled BMSCs. |
| [Keywords] Mesenchymal stem cells；Magnetic resonance imaging；Staining and labeling；Gadolinium DTPA；Animals, laboratory |
GENG Kuan Medical Imaging Center, the 2nd Affiliated Hospital, Medical College of Shantou University, Shantou 515041, China
JIA Yan-long Medical Imaging Center, the 2nd Affiliated Hospital, Medical College of Shantou University, Shantou 515041, China
HUANG De-xiao Medical Imaging Center, the 2nd Affiliated Hospital, Medical College of Shantou University, Shantou 515041, China
YI Mei-zhi Medical Imaging Center, the 2nd Affiliated Hospital, Medical College of Shantou University, Shantou 515041, China
YANG Zhong-xian Medical Imaging Center, the 2nd Affiliated Hospital, Medical College of Shantou University, Shantou 515041, China
LIN Yan Medical Imaging Center, the 2nd Affiliated Hospital, Medical College of Shantou University, Shantou 515041, China
WU Ren-hua * Medical Imaging Center, the 2nd Affiliated Hospital, Medical College of Shantou University, Shantou 515041, China
*Correspondence to: Wu RH, Email: rhwu@stu.edu.cn
Conflicts of interest None.
| Received 2013-08-22 |
| Accepted 2013-10-10 |
| DOI: 10.3969/j.issn.1674-8034.2013.06.007 |
| DOI:10.3969/j.issn.1674-8034.2013.06.007. |
[1]
Vaz, AP, Ponnusamy MP, Batra SK. Cancer stem cells and therapeutic targets: an emerging field for cancer treatment. Drug Deliv Transl Res, 2013, 3(2): 113-120.
[2]
Barry F, Murphy M. Mesenchymal stem cells in joint disease and repair. Nat Rev Rheumatol, 2013, 9(10): 584-594.
[3]
Detante O, Valable S, De Fraipont F, et al. Magnetic resonance imaging and fluorescence labeling of clinical-grade mesenchymal stem cells without impacting their phenotype: study in a rat model of stroke. Stem cells translational medicine, 2012, 1(4): 333-340.
[4]
Chen XW, Zhu DJ, Ju YL, Zhou SF. Therapeutic effect of transplanting magnetically labeled bone marrow stromal stem cells in a liver injury rat model with 70%-hepatectomy. Med Sci Monit, 2012, 18(10): BR375-382.
[5]
Ittrich H, Lange C, Tögel F, et al. In vivo magnetic resonance imaging of iron oxide-labeled, arterially-injected mesenchymal stem cells in kidneys of rats with acute ischemic kidney injury: detection and monitoring at 3 T. J Magne Reson Imaging, 2007, 25(6): 1179-1191.
[6]
Cao J, Wang YN, Shi XL, et al. In vivo and in vitro imaging tracing of dual-labeled bone mesenchymal stem cells transplanted into myocardium of F344 rats. Zhongguo yi xue ke xue yuan xue bao, 2012, 34(5): 474-479.
[7]
[8]
王建东，胡秋菊，朱飞鹏，等．铁蛋白与SPIO在磁共振细胞活体示踪中的增效作用．磁共振成像, 2010, 1(4): 299-304.
[9]
Shen J, Cheng LN, Zhong XM, et al. Efficient in vitro labeling rabbit neural stem cell with paramagnetic Gd-DTPA and fluorescent substance. Eur J Radiol, 2010, 75(3): 397-405.
[10]
Shyu WC, Chen CP, Lin SZ, et al. Efficient tracking of non-iron-labeled mesenchymal stem cells with serial MRI in chronic stroke rats. Stroke, 2007, 38(2): 367-374.
[11]
Corti R, Badimon J, Mizsei G, et al. Real time magnetic resonance guided endomyocardial local delivery. Heart, 2005, 91(3): 348-353.
[12]
Walczak P, Kedziorek DA, Gilad AA, et al. Applicability and limitations of MR tracking of neural stem cells with asymmetric cell division and rapid turnover: the case of the shiverer dysmyelinated mouse brain. Magn Reson Med, 2007, 58(2): 261-269.
[13]
Modo M, Cash D, Mellodew K, et al. Tracking transplanted stem cell migration using bifunctional, contrast agent-enhanced, magnetic resonance imaging. Neuroimage, 2002, 17(2): 803-811.
[14]
陈长青，王小宜，陈晨，等. SPIO标记脂肪干细胞移植治疗大鼠脑梗死的磁共振示踪成像研究．磁共振成像, 2010, 1(1): 50-54.
[15]
Chen XG, Wang N, Yao JH, et al. Gene delivery to mice spermatogenic stem cells by Effectene. Chin Med J (Engl), 2004,117(4): 630-632.
[16]
Shyu WC, Chen CP, Lin SZ, et al. Efficient tracking of non-iron-labeled mesenchymal stem cells with serial MRI in chronic stroke rats. Stroke, 2007, 38(2): 367-374.
[17]
Syková E, Jendelová P, Urdzíková L, et al. Bone marrow stem cells and polymer hydrogels: two strategies for spinal cord injury repair. Cell Mol Neurobiol, 2006, 26(7-8): 1113-1129.
[18]
Hoehn M, Küstermann E, Blunk J, et al. Monitoring of implanted stem cell migration in vivo: a highly resolved in vivo magnetic resonance imaging investigation of experimental stroke in rat. Proc Natl Acad Sci USA, 2002, 99(25): 16267-16272.
[19]
Guenoun J, Koning GA, Doeswijk G, et al. Cationic Gd-DTPA liposomes for highly efficient labeling of mesenchymal stem cells and cell tracking with MRI. Cell Transplant, 2012, 21(1): 191-205.
[20]
Tran LA, Krishnamurthy R, Muthupillai R, et al. Gadonanotubes as magnetic nanolabels for stem cell detection. Biomaterials, 2010, 31(36): 9482-9491.
[21]
Modo M, Mellodew K, Cash D, et al. Mapping transplanted stem cell migration after a stroke: a serial, in vivo magnetic resonance imaging study. Neuroimage, 2004, 21(1): 311-317.
[22]
Daldrup-Link HE, Rudelius M, Oostendorp RA, et al. Targeting of hematopoietic progenitor cells with MR contrast agents1. Radiology, 2003, 228(3): 760-767.
