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• Original Article •
9.4 T MR spectroscopy detection of mesenchymal stem cells into osteoblasts metabolism characteristics
HONG Bi-kai  DAI Hai-yang  XIAO Yu-ye  WU Ren-hua 

DOI:10.3969/j.issn.1674-8034.2013.06.008.


[Abstract] Objective: To explore the metabolite profile of mesenchymal stem cells (MSCs) underwent osteogenic differentiation using 9.4 T high resolution MR spectroscopy.Materials and Methods: Human umbilical cord mesenchymal stem cells were cultured and collected. MSCs were induced osteogenic differentiation for 2 weeks and cell metabolite extractions were prepared by methanol-chloroform (M/C) method. Cell extractions were then analyzed on a 9.4 T MRS device.Results: After osteogenic inducement, the fatty acid metabolite peak at 2.02 ppm and 2.25 ppm obviously decreased, and there appeared a new metabolite peak at about 1.77 ppm, which is most probably representative of leucine.Conclusions: There are some specific characteristics on MRS of MSCs underwent osteogenic differentiation. The new arised peak at 1.77 ppm may serve as the biomarker for mesenchymal stem cells osteogenic differentiation.
[Keywords] Mesenchymal stem cells；Magnetic resonance imaging；Staining and labeling；Gadolinium DTPA；Animals, laboratory

HONG Bi-kai Medical Imaging Center, the 2nd Affiliated Hospital, Medical College of Shantou University, Shantou 515041, China

DAI Hai-yang Department of Medical Imaging, Huizhou Municipal Central Hospital, Huizhou 516001, China

XIAO Yu-ye Medical Imaging Center, the 2nd Affiliated Hospital, Medical College of Shantou University, Shantou 515041, China

WU Ren-hua * Medical Imaging Center, the 2nd Affiliated Hospital, Medical College of Shantou University, Shantou 515041, China

*Correspondence to: Wu RH, Email: rhwu@stu.edu.cn

Conflicts of interest   None.

Received  2013-08-22
Accepted  2013-10-08
DOI: 10.3969/j.issn.1674-8034.2013.06.008
DOI:10.3969/j.issn.1674-8034.2013.06.008.

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