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• Original Article •
Biological characteristics and MR imaging features of SPIO labeled BMSCs in vitro
ZHAO Jiang-min  LIU Jia  LIN Yan-bing  ZONG Gen-lin  WU Li-zhong  QIAN Hai-shan  YOU Jian-xiong 

DOI:10.3969/j.issn.1674-8034.2014.02.011.


[Abstract] Objective: To observe the biological characteristics of rat bone marrow mesenchymal stem cells (BMSCs) labeled by superparamagnetic iron oxide (SPIO) in vitro, and to observe the magnetic resonance imaging (MRI) features of labeled BMSCs in vitro.Materials and Methods: BMSCs were isolated and cultured by plastic adherence method. The BMSCs of the fourth passage were identified by specific surface antigens by flow cytometr. BMSCs were labeled with SPIO of different concentrations. Trypan blue staining and MTT test to evaluate the effects of SPIO on cell labeling efficiency, cell viability and cell proliferation. The MRI imaging features of labeled BMSCs were observed by MRI scanning.Results: The labeling efficiency of all SPIO groups was about 99%. BMSCs labeled with SPIO-PLL at the concentrations (25, 50 μg/ml SPIO, and 0.75 μg/ml PLL), Cell viability rates were about 95%. While at the concentration of SPIO (75, 100 μg/ml), cell viability rate was 92.7% and cell proliferation was significantly inhibited (P<0.05). On MRI imaging, with the concentration of SPIO increasing, signal intensity decreased gradually. When SPIO concentration ≥50 μg/ml, T2 signal intensity of labeled cells compared with normal BMSCs were significantly different (P<0.05).Conclusions: The appropriate concentration of SPIO could not influence on cells biological characteristics. MRI scanning can show the SPIO nanoparticles labeled rat BMSCs significantly and effectively.
[Keywords] Superparamagnetic iron oxides；Mesenchymal stem cells；Biological markers；Magnetic resonance image

ZHAO Jiang-min Department of Medical Imaging, the Third Peopl’s Hospital Affiliated to Shanghai Jiaotong University School of Medicine. Shanghai 201999, China

LIU Jia Department of Medical Imaging, Oriental Hospital of Shanghai City. Shanghai 200120, China

LIN Yan-bing Department of Medical Imaging, the Third Peopl’s Hospital Affiliated to Shanghai Jiaotong University School of Medicine. Shanghai 201999, China

ZONG Gen-lin Department of Medical Imaging, Oriental Hospital of Shanghai City. Shanghai 200120, China

WU Li-zhong Department of Medical Imaging, the Third Peopl’s Hospital Affiliated to Shanghai Jiaotong University School of Medicine. Shanghai 201999, China

QIAN Hai-shan Department of Medical Imaging, the Third Peopl’s Hospital Affiliated to Shanghai Jiaotong University School of Medicine. Shanghai 201999, China

YOU Jian-xiong* Department of Medical Imaging, the Third Peopl’s Hospital Affiliated to Shanghai Jiaotong University School of Medicine. Shanghai 201999, China

*Correspondence to: You JX, E-mail: 15921013455@163.com

Conflicts of interest   None.

Received  2013-11-05
Accepted  2014-01-04
DOI: 10.3969/j.issn.1674-8034.2014.02.011
DOI:10.3969/j.issn.1674-8034.2014.02.011.

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