Share：
Share this content in WeChat
X
[Chinese][PDF]90696
• Original Article •
Biological characteristics and MR imaging features of SPIO labeled BMSCs in vitro
ZHAO Jiang-min  LIU Jia  LIN Yan-bing  ZONG Gen-lin  WU Li-zhong  QIAN Hai-shan  YOU Jian-xiong 

DOI:10.3969/j.issn.1674-8034.2014.02.011.


[Abstract] Objective: To observe the biological characteristics of rat bone marrow mesenchymal stem cells (BMSCs) labeled by superparamagnetic iron oxide (SPIO) in vitro, and to observe the magnetic resonance imaging (MRI) features of labeled BMSCs in vitro.Materials and Methods: BMSCs were isolated and cultured by plastic adherence method. The BMSCs of the fourth passage were identified by specific surface antigens by flow cytometr. BMSCs were labeled with SPIO of different concentrations. Trypan blue staining and MTT test to evaluate the effects of SPIO on cell labeling efficiency, cell viability and cell proliferation. The MRI imaging features of labeled BMSCs were observed by MRI scanning.Results: The labeling efficiency of all SPIO groups was about 99%. BMSCs labeled with SPIO-PLL at the concentrations (25, 50 μg/ml SPIO, and 0.75 μg/ml PLL), Cell viability rates were about 95%. While at the concentration of SPIO (75, 100 μg/ml), cell viability rate was 92.7% and cell proliferation was significantly inhibited (P<0.05). On MRI imaging, with the concentration of SPIO increasing, signal intensity decreased gradually. When SPIO concentration ≥50 μg/ml, T2 signal intensity of labeled cells compared with normal BMSCs were significantly different (P<0.05).Conclusions: The appropriate concentration of SPIO could not influence on cells biological characteristics. MRI scanning can show the SPIO nanoparticles labeled rat BMSCs significantly and effectively.
[Keywords] Superparamagnetic iron oxides；Mesenchymal stem cells；Biological markers；Magnetic resonance image

ZHAO Jiang-min Department of Medical Imaging, the Third Peopl’s Hospital Affiliated to Shanghai Jiaotong University School of Medicine. Shanghai 201999, China

LIU Jia Department of Medical Imaging, Oriental Hospital of Shanghai City. Shanghai 200120, China

LIN Yan-bing Department of Medical Imaging, the Third Peopl’s Hospital Affiliated to Shanghai Jiaotong University School of Medicine. Shanghai 201999, China

ZONG Gen-lin Department of Medical Imaging, Oriental Hospital of Shanghai City. Shanghai 200120, China

WU Li-zhong Department of Medical Imaging, the Third Peopl’s Hospital Affiliated to Shanghai Jiaotong University School of Medicine. Shanghai 201999, China

QIAN Hai-shan Department of Medical Imaging, the Third Peopl’s Hospital Affiliated to Shanghai Jiaotong University School of Medicine. Shanghai 201999, China

YOU Jian-xiong* Department of Medical Imaging, the Third Peopl’s Hospital Affiliated to Shanghai Jiaotong University School of Medicine. Shanghai 201999, China

*Correspondence to: You JX, E-mail: 15921013455@163.com

Conflicts of interest   None.

Received  2013-11-05
Accepted  2014-01-04
DOI: 10.3969/j.issn.1674-8034.2014.02.011
DOI:10.3969/j.issn.1674-8034.2014.02.011.

[1]
Pittenger MF, Mackay AM, Beck SC, et al. Multilineage potential of adult human mesenchymal stem cells. Science, 1999, 284(5411):143-147.
[2]
Petersen BE, Bowen WC, Patrene KD, et al. Bone marrow as a potential source of hepatic oval cells. Science, 1999, 284(5417):1168-1170.
[3]
Sanchez-Ramos J, Song S, Cardozo-Pelaez F, et al. Adult bone marrow stromal cells differentiate intoneural cells in vitro. ExpNeurol, 2000,164(2): 247-56.
[4]
Salem HK, Thiemermann C. Mesenchymal stromal cells: current understanding and clinical status. Stem Cells, 2010, 28(3): 585-596.
[5]
Chen A, Siow B, Blamire AM, et al. Transplantation of magnetically labeled mesenchymal stem cells in a model of perinatal brain injury. Stem Cell Res, 2010, 5(3): 255-266.
[6]
Hokai M, Kuroda S, Shichinohe H, et al. Bone marrow stromal cells protect and repair damaged neurons through multiple mechanisms. Neurosci Res, 2008, 86(5): 1024-1035.
[7]
Bulte JW, Kraitchman DL. Monitoring cell therapy using iron oxide MR contrast agents. Curr Pharm Biotechnol, 2004, 5(6): 567-584
[8]
曹剑，王怡宁，孔令燕,等.活体MR成像监测移植干细胞治疗SD大鼠心肌梗死并评价左心功能的可行性研究.磁共振成像, 2011,2(5): 337-342.
[9]
Frank JA, Miller BR, Arbab AS, et al. Clinically app licablelabeling of mammalian and stem cells by combining superparamagnetic iron oxides and transfection agents. Radiology, 2003, 228(4): 480-487.
[10]
Ittrich H, Lange C, Togel F, et al. In vivo magnetic resonance imaging of iron oxide-labeled, arterially-injected mesenchymal stem cells in kidneys of rats with acute ischemic kidney injury: detection and monitoring at 3 T. J Magn Reson Imaging, 2007, 25(6): 1179-1191.
[11]
Smirnov P, Lavergne E, Gazeau F, et al. In vivo cellular imaging of lymphocyte trafficking by MRI: a tumor model approach to cell-based anticancer therapy. Magn Reson Med, 2006, 56(3): 498-508.
[12]
Neri M, Madema C, Cavazzin C, et al. Eficient in vitro labeling of human neural precursor cells with superparamagnetic iron oxide particles: relevance for in vivo cell tracking. Stem Cells, 2008, 26(2):505-516.
[13]
Daldrup-Link HE, Rudelius M, Oostendorp RA, et al. Targeting of hematopoietic progenitor cells with MR contrast agents. Radiology, 2003, 228(3): 760-767.
[14]
Arbab AS, Bashaw LA, Miller BR et al. Characterization of biophysical and metabolic properties of cells labeled with superparamagnetic iron oxide nanoparticles and transfection agent for cellular MR imaging. Radiology, 2003, 229(3): 838-846.
[15]
Guo RM, Cao N, Zhang F，et al. Controllable labelling of stem cells with a novel superparamagnetic iron oxide-loaded cationic nanovesicle for MR imaging. Eur Radiol, 2012, 22(11): 2328-3237.
[16]
Schafer R, Kehlbach R, Wiskirchen J, et al. Transferrin receptor upregulation: in vitro labeling of rat mesenchymal stem cells with superparamagnetic iron oxide. Radiology, 2007, 244(2): 514-523.
[17]
Arbab AS, Wilson LB, Ashari P, et al. A model of lysosomal metabolism of dextran coated superparamagnetic iron oxide (SPIO) nanoparticles: implications for cellular magnetic resonance imaging. NMR Biomed, 2005, 18(6): 383-389.
[18]
Ben-Hur T, van Heeswijk RB, Einstein O, et al. Serial in vivo MR tracking of magnetically labeled neural spheres transplanted in chronic EAE mice. Magn Reson Med, 2007, 57(1): 164-171.
[19]
Emerit J, Beaumont C, Trivin F. Iron metabolism, free radicals, and oxidative injury. Biomed Pharmacotherapy, 200l, 55(6): 333-339.
[20]
高振华，胡晓书，蔡华松,等. SPIO标记兔BMSCs的生物学特性及体外MRI显像研究.中国CT和MRI杂志, 2012, 10(5): 1-4.
[21]
Lee JH, Jung MJ, Hwang YH, et al. Heparin-coated superparamagnetic iron oxide for in vivo MR imaging of human MSCs. Biomaterials, 2012, 3(35): 4861-4871.
[22]
Rosenberg JT, Sellgren KL, Sachi-Kocher A, et al. Magnetic resonance contrast and biological effects of intracellular superparamagnetic iron oxides on human mesenchymal stem cells with long-term culture and hypoxic exposure. Cytotherapy, 2013, 15(3): 307-322.
[23]
王建东，胡秋菊，朱飞鹏,等.铁蛋白与SPIO在磁共振细胞活体示踪中的增效作用.磁共振成像, 2010, 1(4): 299-304.

PREV Relationships between 3.0 T MRI features and prognostic factors of patients in breast invasive ductal carcinoma
NEXT Clinical application of three-dimensional MR angiography using time-resolved imaging of contrast kinetics
  


LINK


Tel & Fax: +8610-67113815    E-mail: editor@cjmri.cn