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• Original Article •
Dynamic MRI research of EPCs tracing rat glioma
LI Ran  FANG Jing-qin  CHEN Xiao  GUO Yu  AI Hua  ZHANG Wei-guo 

DOI:10.3969/j.issn.1674-8034.2014.04.012.


[Abstract] Objective: The aim of this study was to investigate the dynamic homing characteristic of exogenous endothelial progenitor cells (EPCs) in rat glioma in vivo to provide an experimental basis for the feasibility of using magnetically labeled EPCs as MRI target tracing vectors.Materials and Methods: Three models of Sprague-Dawley at glioma (48 rats in total) were established as control group, experimental group and fake experimental group, In the control group and experimental group, orthotopic transplantation of C6 glioma cells was performed, compared to above groups, only the brain was punctured and no C6 glioma cells was performed. 8 days after the models were established, in the control group and experimental group, 2×106 USPIO-labeled EPCs were transplanted via the tail vein. Magnetic resonance imaging and perfusion-weighted imaging were performed on several days. The conventional MR imaging, including spin echo and gradin-echo sequence, were performed at before and 1, 3, 5, 7 days after transplantation on each group to observe the signal change of the diseased region on T2WI, SWI and T2 value. Tumors were excised from experimental rat of every group at each examined point to make pathological assay. Prussian blue staining represent the distribution of USPIO-EPCs in the tumor. CD34+, SDF-1, MMP-9 and VEGF staining were used to observe the distribution relationship between the Prussian blue staining positive cells and these antibody.Results: In fake experimental group, the MRI signal was similar all the time. The T2 value-time curve was straight. In control group, tumor size developed gradually but the no signal changed, in the experimental group, hypointense areas were detected at the periphery of the tumor on the first day after transplantation of EPCs, and much more hypointense areas were observed inside the tumor over time. The T2 value-time curve was downtrending. There were a little blue-stained cells in fake experimental group, and several blue-stained cells were observed at the the periphery of the tumor on the first day after transplantation of EPCs, and migrated in the center of the tumor gradually. At the sites of blued stained cells, both SDF-1 and MMP-9 were strongly positive, they showed generalized expression in the periphery of the tumor in the early stage, the number of positive antibodies gradually increased in the center of the tumor in the 7 day after transplantation of EPCs, conversely. There was no significant association of blue stained cell localization and VEGF expression in tumor cells.Conclusions: The homing change procedure of USPIO-EPCs in rat glioma can observed by MRI in vivo, there was significant correlation between USPIO-EPCs localization and the change of SDF-1 and MMP-9 in different areas, the antibody SDF-1 and MMP-9 may be one of the molecular biology of EPCs migration.
[Keywords] Glioma；Stem cell；Endothelial cells；Magnetic resonance imaging；Animal, laboratory

LI Ran Department of Radiology, Institute of Surgery Research, Daping Hospital, Third Military Medical University, Chongqing 400042, China

FANG Jing-qin Department of Radiology, Institute of Surgery Research, Daping Hospital, Third Military Medical University, Chongqing 400042, China

CHEN Xiao Department of Radiology, Institute of Surgery Research, Daping Hospital, Third Military Medical University, Chongqing 400042, China

GUO Yu Department of Radiology, Institute of Surgery Research, Daping Hospital, Third Military Medical University, Chongqing 400042, China

AI Hua National Engineering Research Center for Biomaterials Sichuan University, Chengdu 610064, China

ZHANG Wei-guo* Department of Radiology, Institute of Surgery Research, Daping Hospital, Third Military Medical University, Chongqing 400042, China

*Correspondence to: Zhang WG, E-mail: wguo.zhang@gmail.com

Conflicts of interest   None.

Received  2014-04-10
Accepted  2014-05-16
DOI: 10.3969/j.issn.1674-8034.2014.04.012
DOI:10.3969/j.issn.1674-8034.2014.04.012.

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