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• Original Article •
T2-weighted image and diffusion tensor imaging of cuprizone-induced demyelination in C57BL/6 mouse model
NIE Ting-ting  SHEN Zhi-wei  GENG Kuan  JIA Yan-long  ZHANG Tao  YAN Gen  WU Ren-hua 

DOI:10.3969/j.issn.1674-8034.2014.05.013.


[Abstract] Objective: Cuprizone (CPZ) mouse model of demyelination was recognized and used to explore multiple sclerosis (MS)-like brain lesions. In this study, we assessed CPZ-treated mice using T2-weighted imaging and diffusion tensor imaging (DTI).Materials and Methods: C57BL/6 mice were scanned with a 7.0 T MRI (Agilent, USA) respectively after four weeks 0.2% CPZ-containing diet (n=10) and regular chow diet (n=10) using fast spin-echo and fast spin-echo DTI sequences. Then we calculated the normalized T2 signal intensity (normalized to the cerebrospinal fluid) and measured fractional anisotropy (FA value), mean diffusivity, axial diffusivity and radial diffusivity of each region. Regions of interest (ROIs) included cerebral cortex (CTX), caudate putamen (CP), hippocampus (HP) and thalamus (TH).Results: Compared with controls, obvious increased normalized T2 signal intensities were observed in CTX, HP and CP (P<0.01), which was mild in TH (P=0.119). Reduced FA values were evident in CTX, HP and CP (P<0.05), which was also mild in TH (P=0.178). In the regions of reduced FA, an increase in mean diffusivity (P<0.05) and radial diffusivity (P<0.05) were found. Obvious decreased axial diffusivity were only observed in CTX (P<0.05).Conclusions: DTI is sensitive to detecting cuprizone mouse model of demyelination, reflecting tissue structure. This study suggests that CTX, HP and CP are more susceptible to cuprizon-induced demyelination compared with TH. Our results also indicate that the decreases of FA may be more likely due to increased radial diffusivity.
[Keywords] Demyelinating diseases；Magnetic resonance imaging；Animal experimentation

NIE Ting-ting Department of Medical Imaging, 2nd Affilicated Hospital, Shantou University Medical College, Shantou 515041, China

SHEN Zhi-wei Department of Medical Imaging, 2nd Affilicated Hospital, Shantou University Medical College, Shantou 515041, China

GENG Kuan Department of Medical Imaging, 2nd Affilicated Hospital, Shantou University Medical College, Shantou 515041, China

JIA Yan-long Department of Medical Imaging, 2nd Affilicated Hospital, Shantou University Medical College, Shantou 515041, China

ZHANG Tao Department of Medical Imaging, 2nd Affilicated Hospital, Shantou University Medical College, Shantou 515041, China

YAN Gen Department of Medical Imaging, 2nd Affilicated Hospital, Shantou University Medical College, Shantou 515041, China

WU Ren-hua * Department of Medical Imaging, 2nd Affilicated Hospital, Shantou University Medical College, Shantou 515041, China; Provincial Key Laboratory of Medical Molecular Imaging, Guangdong, Medical College of Shantou University, Shantou 515041, China

*Correspondence to: Wu RH, E-mail: cjr.wurenhua@vip.163.com

Conflicts of interest   None.

Received  2014-04-10
Accepted  2014-07-20
DOI: 10.3969/j.issn.1674-8034.2014.05.013
DOI:10.3969/j.issn.1674-8034.2014.05.013.

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