Share：
Share this content in WeChat
X
[Chinese][PDF]70370
• Original Article •
Spatiotemporal evolution of blood brain barrier damage associated with changes of brain metabolites following ischemia onset
ZHANG Tao  NIE Ting-ting  JIA Yan-long  YI Mei-zhi  DAI Zhuo-zhi  YAN Gen  WU Ren-hua 

DOI:10.3969/j.issn.1674-8034.2014.05.014.


[Abstract] Objectives: Blood brain barrier (BBB) damage that occurs within the thrombolytic time window is increasingly appreciated to negatively impact the safety and efficacy profiles of thrombolytic therapy for ischemic stroke. Here, we investigated the topographical distribution of BBB damage and its association with tissue injury within the first 3 h after ischemia onset and the related roles of neurochemical metabolites profiles in this process.Materials and Methods: Here, using contrast-enhanced magnetic resonance imaging (CE-MRI) and single-voxel localization technique, we assessed BBB disruption onset time and related neurochemical alteration in rats following middle cerebral artery occlusion (MCAO) for 1, 2 and 3 h. Proton metabolites were quantified using LCModel software.Results: In the 1.5 h MCAO group, after 0.5 h reperfusion, abnormal hyperintense signals were seen in both lateral ventricles and the central canal in the CE-T1WI image, and expanded with time to other MCA regions, including the ventromedial striatum in the 2 h MCAO group, after 0.5 h reperfusion. The concentrations of glutamate+glutamine (Glu+Gln), taurine (Tau) and creatine+phosphocreatine (Cr+PCr) increased gradually within the first 2 h (P<0.05, respectively), and then decreased (P<0.05, respectively) in the following 1 h after ischemia onset, as evaluated by 1H-MRS. Other metabolites observed in this study did not show significant changes over the time.Conclusions: Our findings suggest Tau, Glu+Gln and Cr+PCr levels may be useful biomarkers to detect BBB damage and relevant events in the ischemic cascade, and predict onset time of BBB disruption to improve the accuracy of acute stroke diagnosis and determine the safety and efficacy of early thrombolytic therapy.
[Keywords] Blood brain barrier；Magnetic resonance spectroscopy；Contrast enhanced T1WI；Ischemia stroke

ZHANG Tao Department of Medical Imaging, 2nd Affilicated Hospital, Shantou University Medical College, Shantou 515041, China

NIE Ting-ting Department of Medical Imaging, 2nd Affilicated Hospital, Shantou University Medical College, Shantou 515041, China

JIA Yan-long Department of Medical Imaging, 2nd Affilicated Hospital, Shantou University Medical College, Shantou 515041, China

YI Mei-zhi Department of Medical Imaging, 2nd Affilicated Hospital, Shantou University Medical College, Shantou 515041, China

DAI Zhuo-zhi Department of Medical Imaging, 2nd Affilicated Hospital, Shantou University Medical College, Shantou 515041, China

YAN Gen Department of Medical Imaging, 2nd Affilicated Hospital, Shantou University Medical College, Shantou 515041, China

WU Ren-hua * Department of Medical Imaging, 2nd Affilicated Hospital, Shantou University Medical College, Shantou 515041, China; Provincial Key Laboratory of Medical Molecular Imaging, Guangdong, Medical College of Shantou University, Shantou 515041, China

*Correspondence to: Wu RH, E-mail: cjr.wurenhua@vip.163.com

Conflicts of interest   None.

Received  2014-05-26
Accepted  2014-07-20
DOI: 10.3969/j.issn.1674-8034.2014.05.014
DOI:10.3969/j.issn.1674-8034.2014.05.014.

[1]
Jauch EC, Saver JL, Adams HP, et al. Guidelines for the early management of patients with acute ischemic stroke a guideline for healthcare professionals from the american heart association american stroke association. Stroke, 2013, 44(3): 870-947.
[2]
Pardridge WM. Blood-brain barrier delivery. Drug Discov Today, 2007, 12(1): 54-61.
[3]
Ballabh P, Braun A, Nedergaard M, et al. The blood-brain barrier: an overview: structure, regulation, and clinical implications. Neurobiol Dis, 2004, 16(1): 1-13.
[4]
Bahn MM, Oser AB, Cross DT, et al. CT and MRI of stroke. J Magnet Reson Imaging, 1996, 6(5): 833-845.
[5]
刘怀军，张岩，杨冀萍．脑缺血的CT、MR表现及其理解．磁共振成像, 2013, 4(1): 47-58.
[6]
Ding G, Jiang Q, Li L, et al. Detection of BBB disruption and hemorrhage by Gd-DTPA enhanced MRI after embolic stroke in rat. Brain Res, 2006, 1114(1):195-203.
[7]
彭娟，罗天友，吕金，等．质子MRS脂质峰升高在颅内疾病鉴别诊断的价值．磁共振成像, 2011, 2(1): 50-54.
[8]
Provencher SW. Automatic quantitation of localizedin vivo1h spectra with lcmodel. NMR Biomed, 2001, 14(2): 260-264.
[9]
Tong D, Yenari M, Albers G, et al. Correlation of perfusion and diffusion weighted MRI with nihss score in acute (< 6.5 hour) ischemic stroke. Neurology, 1998, 50(4): 864-869.
[10]
Neumann-Haefelin T, Wittsack HJ, Wenserski F, et al. Diffusion-and perfusion-weighted MRI the DWI/PWI mismatch region in acute stroke. Stroke, 1999, 30(2): 1591-1597.
[11]
Rordorf G, Koroshetz WJ, Copen WA, et al. Regional ischemia and ischemic injury in patients with acute middle cerebral artery stroke as defined by early diffusion-weighted and perfusion-weighted MRI. Stroke, 1998, 29(5): 939-943.
[12]
Schellinger P, Bryan R, Caplan L, et al. Evidence-based guideline: the role of diffusion and perfusion MRI for the diagnosis of acute ischemic stroke report of the therapeutics and technology assessment subcommittee of the american academy of neurology. Neurology, 2010, 75(1): 177-185.
[13]
Tofts PS, Kermode AG. Measurement of the blood-brain barrier permeability and leakage space using dynamic MR imaging. Magnet Reson Med, 1991, 17(2): 357-367.
[14]
Lo EH, Pan Y, Matsumoto K, et al. Blood-brain barrier disruption in experimental focal ischemia: comparison between in vivo MRI and immunocytochemistry. Magnet Reson Imaging1994, 12(3): 403-411.
[15]
Wu L, Tang Z, Sun X, et al. Metabolic changes in the visual cortex of binocular blindness macaque monkeys: a proton magnetic resonance spectroscopy study. PLoS One, 2013, 8(11): e80073.
[16]
延根，赵大威，戴卓智，等．评估量化超急性期缺血性脑卒中发病时间窗的研究．磁共振成像, 2014, 5(1): 74-79.
[17]
Kang YS, Ohtsuki S, Takanaga H, et al. Regulation of taurine transport at the blood-brain barrier by tumor necrosis factor-α , taurine and hypertonicity. J Neurochem, 2002, 83(5): 1188-1195.

PREV T2-weighted image and diffusion tensor imaging of cuprizone-induced demyelination in C57BL/6 mouse model
NEXT A case of extraventricular neurocytoma of the cerebellopontine angle area
  


LINK


Tel & Fax: +8610-67113815    E-mail: editor@cjmri.cn