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• Technical Article •
Study of a new method for imaging of GABA based on chemical exchange saturation transfer using a 7.0 T MR scanner
ZHANG Tao  JIA Yan-long  NIE Ting-ting  YI Mei-zhi  DAI Zhuo-zhi  YAN Gen  WU Ren-hua 

DOI:10.3969/j.issn.1674-8034.2015.05.013.


[Abstract] Objective: γ-aminobutyric acid (GABA) is an important inhibitory neurotransmitter in the brain. In this study, we developed a novel MRI technique to measure GABA based on chemical exchange saturation transfer (CEST).Materials and Methods: All the MRI studies were performed in a 7.0 T Agilent MRI scanner. Z-spectra of GABA and other metabolites (glutamate, myo-inositol, creatine and choline) were acquired at 37° and pH 7.0, respectively. CEST images of phantoms with different concentrations of GABA solutions and other metabolites were collected to investigate the concentration dependent CEST effect of GABA and the potential contributions from other brain metabolites.Results: The CEST effect of GABA was observed at 2.75 ppm downfield from bulk water. The CEST effect of GABA increased with B1 amplitude and kept steady after the B1 reached 6.0 μT. The CEST effect of GABA was proportional to the GABA concentration in vitro. Except for a small contribution from glutamate, all other metabolites contributed negligible CEST effects to GABACEST at 2.75 ppm.Conclusions: These findings demonstrate the feasibility and potential to map changes in GABA concentration using CEST with a optimal B1 amplitude, which has excellent spatial and temporal resolution.
[Keywords] Gamma-aminobutyric acid；Magnetic resonance imaging；Metabolism

ZHANG Tao Department of Medical Imaging, 2nd Affilicated Hospital, Shantou University Medical College, Shantou 515041, China

JIA Yan-long Department of Medical Imaging, 2nd Affilicated Hospital, Shantou University Medical College, Shantou 515041, China

NIE Ting-ting Department of Medical Imaging, 2nd Affilicated Hospital, Shantou University Medical College, Shantou 515041, China

YI Mei-zhi Department of Medical Imaging, 2nd Affilicated Hospital, Shantou University Medical College, Shantou 515041, China

DAI Zhuo-zhi Department of Medical Imaging, 2nd Affilicated Hospital, Shantou University Medical College, Shantou 515041, China

YAN Gen Department of Medical Imaging, 2nd Affilicated Hospital, Shantou University Medical College, Shantou 515041, China

WU Ren-hua* Department of Medical Imaging, 2nd Affilicated Hospital, Shantou University Medical College, Shantou 515041, China; Provincial Key Laboratory of Medical Molecular Imaging, Guangdong, Medical College of Shantou University, Shantou 515041, China

*Correspondence to: Wu RH, E-mail: rhwu@stu.edu.cn

Conflicts of interest   None.

Received  2014-05-10
Accepted  2015-03-26
DOI: 10.3969/j.issn.1674-8034.2015.05.013
DOI:10.3969/j.issn.1674-8034.2015.05.013.

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