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• Original Article •
The feasibility of IDEAL-IQ quantitative evaluation of vertebral fat fraction content in rabbit models of diabetes mellitus
HU Lei  ZHA Yun-fei  LIN Yuan  XING Dong  GONG Wei  WANG Ke-jun  YAN Li-yong  WANG Jiao 

DOI:10.3969/j.issn.1674-8034.2015.12.012.


[Abstract] Objective: To use the IDEAL-IQ technique to estimate vertebral fat fraction(FF) in alloxan-induced diabetic rabbits.Materials and Methods: Twelve young New Zealand white rabbits were randomly assigned to alloxan-induced diabetic group(n=6) and control group(n=6). The rabbits in alloxan-induced diabetic group were injected with a total amount of 100 mg/kg of alloxan. Both of the rabbits in alloxan-induced diabetic and control group underwent magnetic resonance imaging (TIWI, T2WI, IDEAL-IQ) at each time point(0, 4, 8, 12, 16 weeks). Each vertebral fat fraction was measured at a standard workstation (AW 4.6; GE Medical Systems). Region of interest (ROI) had a shape of rectangle and they were drawn manually. HE staining was performed.Results: Slight increase of the signal of the lumbar vertebral bodies was found at the T1WI and T2WI in the alloxan-induced diabetic group at the week of 16. Compared to the control group, the alloxan-induced diabetic group showed the reduce of bone cells and increase of the fat cells. The alloxan-induced diabetic group has statistically differences in vertebral fat fraction at each time point(F=50.387, P<0.01). The control group has no statistical differences in vertebral fat fraction at each time point(F=1.477, P>0.05). There is statistical difference in vertebral fat fraction between alloxan-induced diabetic and control group at the week of 16(t=-10.726, P<0.05). A significant positive correlation is found between vertebral fat fraction and fat cell count(r=0.925, P<0.05).Conclusion: IDEAL-IQ technique can quantify the vertebral fat fraction in alloxan-induced diabetic rabbits.
[Keywords] Diabetes mellitus, Experimental；Vertebral marrow；Quantification；Magnetic resonance imaging；Laboratory animal science；Rabbits

HU Lei Department of Radiology, Renmin Hospital of Wuhan University, Wuhan 430060, China

ZHA Yun-fei* Department of Radiology, Renmin Hospital of Wuhan University, Wuhan 430060, China

LIN Yuan Department of Radiology, Renmin Hospital of Wuhan University, Wuhan 430060, China

XING Dong Department of Radiology, Renmin Hospital of Wuhan University, Wuhan 430060, China

GONG Wei Department of Radiology, Renmin Hospital of Wuhan University, Wuhan 430060, China

WANG Ke-jun Department of Radiology, Renmin Hospital of Wuhan University, Wuhan 430060, China

YAN Li-yong Department of Radiology, Renmin Hospital of Wuhan University, Wuhan 430060, China

WANG Jiao Department of Radiology, Renmin Hospital of Wuhan University, Wuhan 430060, China

*Correspondence to: Zha YF, E-mail: zhayunfei999@126.com

Conflicts of interest   None.

ACKNOWLEDGMENTS  This work was part of natural science foundation of Hubei province No. 2013CFB242 scientific research project of health department of Hubei No. JX6B68
Received  2015-09-11
Accepted  2015-10-20
DOI: 10.3969/j.issn.1674-8034.2015.12.012
DOI:10.3969/j.issn.1674-8034.2015.12.012.

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