Share：
Share this content in WeChat
X
[Chinese][PDF]87895
• Original Article •
The feasibility of IDEAL-IQ quantitative evaluation of vertebral fat fraction content in rabbit models of diabetes mellitus
HU Lei  ZHA Yun-fei  LIN Yuan  XING Dong  GONG Wei  WANG Ke-jun  YAN Li-yong  WANG Jiao 

DOI:10.3969/j.issn.1674-8034.2015.12.012.


[Abstract] Objective: To use the IDEAL-IQ technique to estimate vertebral fat fraction(FF) in alloxan-induced diabetic rabbits.Materials and Methods: Twelve young New Zealand white rabbits were randomly assigned to alloxan-induced diabetic group(n=6) and control group(n=6). The rabbits in alloxan-induced diabetic group were injected with a total amount of 100 mg/kg of alloxan. Both of the rabbits in alloxan-induced diabetic and control group underwent magnetic resonance imaging (TIWI, T2WI, IDEAL-IQ) at each time point(0, 4, 8, 12, 16 weeks). Each vertebral fat fraction was measured at a standard workstation (AW 4.6; GE Medical Systems). Region of interest (ROI) had a shape of rectangle and they were drawn manually. HE staining was performed.Results: Slight increase of the signal of the lumbar vertebral bodies was found at the T1WI and T2WI in the alloxan-induced diabetic group at the week of 16. Compared to the control group, the alloxan-induced diabetic group showed the reduce of bone cells and increase of the fat cells. The alloxan-induced diabetic group has statistically differences in vertebral fat fraction at each time point(F=50.387, P<0.01). The control group has no statistical differences in vertebral fat fraction at each time point(F=1.477, P>0.05). There is statistical difference in vertebral fat fraction between alloxan-induced diabetic and control group at the week of 16(t=-10.726, P<0.05). A significant positive correlation is found between vertebral fat fraction and fat cell count(r=0.925, P<0.05).Conclusion: IDEAL-IQ technique can quantify the vertebral fat fraction in alloxan-induced diabetic rabbits.
[Keywords] Diabetes mellitus, Experimental；Vertebral marrow；Quantification；Magnetic resonance imaging；Laboratory animal science；Rabbits

HU Lei Department of Radiology, Renmin Hospital of Wuhan University, Wuhan 430060, China

ZHA Yun-fei* Department of Radiology, Renmin Hospital of Wuhan University, Wuhan 430060, China

LIN Yuan Department of Radiology, Renmin Hospital of Wuhan University, Wuhan 430060, China

XING Dong Department of Radiology, Renmin Hospital of Wuhan University, Wuhan 430060, China

GONG Wei Department of Radiology, Renmin Hospital of Wuhan University, Wuhan 430060, China

WANG Ke-jun Department of Radiology, Renmin Hospital of Wuhan University, Wuhan 430060, China

YAN Li-yong Department of Radiology, Renmin Hospital of Wuhan University, Wuhan 430060, China

WANG Jiao Department of Radiology, Renmin Hospital of Wuhan University, Wuhan 430060, China

*Correspondence to: Zha YF, E-mail: zhayunfei999@126.com

Conflicts of interest   None.

ACKNOWLEDGMENTS  This work was part of natural science foundation of Hubei province No. 2013CFB242 scientific research project of health department of Hubei No. JX6B68
Received  2015-09-11
Accepted  2015-10-20
DOI: 10.3969/j.issn.1674-8034.2015.12.012
DOI:10.3969/j.issn.1674-8034.2015.12.012.

[1]
Li X, Kuo D, Schafer AL, et al. Quantification of vertebral bone marrow fat content using 3 Tesla MR spectroscopy: reproducibility, vertebral variation, and applications in osteoporosis. J Magn Reson Imaging, 2011, 33(4): 974-979.
[2]
Reeder SB, Cruite I, Hamilton G, et al. Quantitative assessment of liver fat with magnetic resonance imaging and spectroscopy. J Magn Reson Imaging, 2011, 34(4): 729-749.
[3]
Alizai H, Nardo L, Karampinos DC, et al. Comparison of clinical semi-quantitative assessment of muscle fat infiltration with quantitative assessment using chemical shift-based water/fat separation in MR studies of the calf of post-menopausal women. Eur Radiol, 2012, 22(7): 1592-1600.
[4]
Oei L, Rivadeneira F, Zillikens MC, et al. Diabetes, diabetic complications, and fracture risk. Curr Osteoporos Rep, 2015, 13(2): 106-115.
[5]
Neglia C, Agnello N, Argentiero A, et al. Increased risk of osteoporosis in postmenopausal women with type 2 diabetes mellitus: a three-year longitudinal study with phalangeal QUS measurements. J Biol Regul Homeost Agents, 2014, 28(4): 733-741.
[6]
Griffith JF, Yeung DK, Ma HT, et al. Bone marrow fat content in the elderly: a reversal of sex difference seen in younger subjects. J Magn Reson Imaging, 2012, 36(1): 225-230.
[7]
Régis-Arnaud A, Guiu B, Walker PM, et al. Bone marrow fat quantification of osteoporotic vertebral compression fractures: comparison of multi-voxel proton MR Bone marrow fat quantification of osteoporotic vertebral compression fractures: comparison of multi-voxel proton MR spectroscopy and chemical-shift gradient-echo MR imaging. Acta Radiol, 2011, 52(9): 1032-1036.
[8]
Kuhn JP, Hernando D, Meffert PJ, et al. Proton-density fat fraction and simultaneous R2* estimation as an MRI tool for assessment of osteoporosis. Eur Radiol, 2013, 23(12): 3432-3439.
[9]
Liu T, Zhao H, Li J, et al. Rosiglitazone attenuates atrial structural remodeling and atrial fibrillation promotion in alloxan-induced diabetic rabbits. Cardiovasc Ther, 2014, 32(4): 178-183.
[10]
Huovinen V, Saunavaara V, Kiviranta R, et al. Vertebral bone marrow glucose uptake is inversely associated with bone marrow fat in diabetic and healthy pigs: [(18)F]FDG-PET and MRI study. Bone, 2014, 61: 33-38.
[11]
张倩，伍建林.多模态功能MRI对糖尿病脑病的研究.磁共振成像, 2015, 6(1): 58-61.
[12]
Baum T, Yap SP, Dieckmeyer M, et al. Assessment of whole spine vertebral bone marrow fat using chemical shift-encoding based water-fat MRI. J Magn Reson Imaging, 2015, 42(4): 1-6.
[13]
Krings A, Rahman S, Huang S, et al. Bone marrow fat has brown adipose tissue characteristics, which are attenuated with aging and diabetes. Bone, 2012, 50(2): 546-552.
[14]
Bredella MA, Torriani M, Ghomi RH, et al. Vertebral bone marrow fat is positively associated with visceral fat and inversely associated with IGF-1 in obese women. Obesity (Silver Spring), 2011, 19(1): 49-53.
[15]
Sakellaridis N. The influence of diabetes mellitus on lumbar intervertebral disk herniation. Surg Neurol, 2006, 66(2): 152-154.
[16]
Morton RP, Tariq F, Levitt MR, et al. Radiographic and clinical outcomes in cavernous carotid fistula with special focus on alternative transvenous access techniques. J Clin Neurosci, 2015, 22(5): 859-864.
[17]
Hines CD, Frydrychowicz A, Hamilton G, et al. T(1) independent, T(2) (*) corrected chemical shift based fat-water separation with multi-peak fat spectral modeling is an accurate and precise measure of hepatic steatosis. J Magn Reson Imaging, 2011, 33(4): 873-881.
[18]
夏吉凯，刘新疆，房清敏,等. IDEAL序列在臂丛神经扫描方案中的对比研究.磁共振成像, 2014, 5(2): 107-110.
[19]
Ergen FB, Gulal G, Yildiz AE, et al. Fat fraction estimation of the vertebrae in females using the T2*-IDEAL technique in detection of reduced bone mineralization level: comparison with bone mineral densitometry. J Comput Assist Tomogr, 2014, 38(2): 320-324.

PREV MRI features of tumor like inflammatory demyelinating disease
NEXT Solitary fibrous tumor of meninges in anterior cranial fossa: case report
  


LINK


Tel & Fax: +8610-67113815    E-mail: editor@cjmri.cn