Share：
Share this content in WeChat
X
[Chinese][PDF]95579
• Original Article •
Evaluation of myocardial fibrosis in miniature pig model of COPD by the 3.0 T magnetic resonance LGE techniques: An experimental study
LIU Zhi  GUO Dandan  LI Chunping  LI Rui 

Cite this article as: Liu Z, Guo DD, Li CP, et al. Evaluation of myocardial fibrosis in miniature pig model of COPD by the 3.0 T magnetic resonance LGE techniques: An experimental study[J]. Chin J Magn Reson Imaging, 2021, 12(8): 49-54. DOI:10.12015/issn.1674-8034.2021.08.010.


[Abstract] Objective To explore the feasibility of 3.0 T cardiovascular magnetic resonance (CMR) late gadolinium enhancement (LGE) techniques in assessing early left ventricular (LV) myocardial fibrosis in miniature pig COPD model, so as to provide imaging basis for detecting early myocardial injury in COPD. Materials andMethods Twenty healthy Bama miniature pigs were divided into COPD model group (16 pigs, COPD model was prepared by protease) and normal control group (4 pigs). Before modeling and at different stages of modeling (4 weeks, 8 weeks and 20 weeks), multimodal CMR scanning was performed. The differences of conventional cardiac parameters among the groups were compared, and the incidence, distribution and types of LV myocardial LGE were analyzed.Results The COPD model of 14 miniature pigs in the model group was successfully constructed. There was no significant difference in baseline data between the model group and the control group in the early and middle stage of modeling (4 weeks and 8 weeks) (P＞ 0.05); however, in the late stage of modeling (20 weeks), respiratory rate and heart rate were significantly increased (P=0.003 and 0.002 respectively); body weight was decreased (P=0.007). There was no significant difference in heart function parameters between groups in the early and middle stages of modeling (P＞0.05). The right ventricular anterior wall (RVAW) (all P＜0.001) was thickened, while the right ventricular end diastolic diameter (RVDd) (P＜0.001) and left ventricular posterior wall (LVPW) (P=0.018) was only thicker than before modeling, left ventricular end diastolic diameter (LVDD) (P=0.05) and left ventricular end diastolic volume (LVEDV) (P=0.005) were decreased than before modeling. The right ventricular ejection fraction (RVEF) was lower than that before modeling and in the control group (P＜0.001 and 0.001); the left ventricular ejection fraction (LVEF) had no statistical difference (P＞0.05). LGE was found in 23 myocardial segments (23/85) of 5 minipigs in the model group after modeling, which was significantly different from that in the control group and before modeling (all P＜0.01). LGE was mainly located in the basal and middle slices of left ventricle, and the distribution was statistically significant (P＜0.05), and the most common type of LGE was intramural type.Conclusions In the early stage of COPD, decreased RVEF, but still in the normal range could be observed. Myocardial fibrosis occurred in a small part of LV myocardium, accompanied with normal LVEF. LGE technology based on CMR could provide imaging basis for early intervention, diagnosis and treatment planning and prognosis evaluation of COPD patients.
[Keywords] chronic obstructive pulmonary disease；cardiovascular magnetic resonance；miniature pigs；late gadolinium enhancement；myocardial injury；fibrosis

LIU Zhi1, 2   GUO Dandan2   LI Chunping2   LI Rui2*  

1 Chongqing Hospital of Traditional Chinese Medicine, Chongqing 400021, China

2 Affiliated Hospital of North Sichuan Medical College, and Sichuan Key Laboratory of Medical Imaging, Nanchong 637000, China

Li R, E-mail: ddtwg_nsmc@163.om

Conflicts of interest   None.

ACKNOWLEDGMENTS This work was part of National Natural Science Foundation of China (No. 81801674); Basic Research on Application of Science and Technology Department of Sichuan Province (No. 2021YJ0242); Scientific Research Project of Sichuan Provincial Health Commission (No. 19PJ201).
Received  2021-03-23
Accepted  2021-05-24
DOI: 10.12015/issn.1674-8034.2021.08.010
Cite this article as: Liu Z, Guo DD, Li CP, et al. Evaluation of myocardial fibrosis in miniature pig model of COPD by the 3.0 T magnetic resonance LGE techniques: An experimental study[J]. Chin J Magn Reson Imaging, 2021, 12(8): 49-54. DOI:10.12015/issn.1674-8034.2021.08.010.

1
Fang L, Gao P, Bao H, et al. Chronic obstructive pulmonary disease in China: a nationwide prevalence study[J]. Lancet Respir Med, 2018, 6(6): 421-430. DOI: 10.1016/S2213-2600(18)30103-6.
2
Wang C, Xu J, Yang L, et al. Prevalence and risk factors of chronic obstructive pulmonary disease in China (the China Pulmonary Health [CPH] study): a national cross-sectional study[J]. Lancet, 2018, 391(10131): 1706-1717. DOI: 10.1016/S0140-6736(18)30841-9.
3
Portillo K, Abad-Capa J, Ruiz-Manzano J. Chronic obstructive pulmonary disease and left ventricle[J]. Arch Bronconeumol, 2015, 51(5): 227-234. DOI: 10.1016/j.arbres.2014.03.012.
4
Alter P, van de Sand K, Nell C, et al. Airflow limitation in COPD is associated with increased left ventricular wall stress in coincident heart failure[J]. Respir Med, 2015, 109(9): 1131-1137. DOI: 10.1016/j.rmed.2015.07.012.
5
SchÄfer M, Humphries S, Stenmark KR, et al. 4D-flow cardiac magnetic resonance-derived vorticity is sensitive marker of left ventricular diastolic dysfunction in patients with mild-to-moderate chronic obstructive pulmonary disease[J]. Eur Heart J Cardiovasc Imaging, 2018, 19(4): 415-424. DOI: 10.1093/ehjci/jex069.
6
Murphy ML, de Soyza N, Thenabadu PN. Quantitation of fibrosis of the heart in chronic obstructive pulmonary disease with and without cor pulmonale[J]. Chest, 1983, 84(5): 535-538. DOI: 10.1378/chest.84.5.535.
7
Özyılmaz S, AlıŞır MF, Serdar OA, et al. The value of coronary artery calcium score in the early diagnosis of coronary artery disease in patients with stable chronic obstructive pulmonary disease[J]. Anatol J Cardiol, 2016, 16(4): 283-289. DOI: 10.5152/AnatolJCardiol.2015.6020.
8
Xia YJ, Sun HY, Jiang L, et al. Evaluation of the effects of right ventricular pressure load on left ventricular myocardial mechanics in patients with chronic obstructive pulmonary disease by ultrasound speckle tracking imaging[J]. Eur Rev Med Pharmacol Sci, 2018, 22(15): 4949-4955. DOI: 10.26355/eurrev_201808_15634.
9
Johns CS, Rajaram S, Capener DA, et al. Non-invasive methods for estimating mPAP in COPD using cardiovascular magnetic resonance imaging[J]. Eur Radiol, 2018, 28(4): 1438-1448.
10
Wang Z, Gu S, Leader JK, et al. Optimal threshold in CT quantification of emphysema[J]. Eur Radiol, 2013, 23(4): 975-984. DOI: 10.1007/s00330-017-5143-y.
11
Crossley D, Renton M, Khan M, et al. CT densitometry in emphysema: a systematic review of its clinical utility[J]. Int J Chron Obstruct Pulmon Dis, 2018, 13: 547-563. DOI: 10.2147/COPD.S143066.
12
Inoue S, Shibata Y, Kishi H, et al. Decreased left ventricular stroke volume is associated with low-grade exercise tolerance in patients with chronic obstructive pulmonary disease[J]. BMJ Open Respir Res, 2017, 4(1): e158. DOI: 10.1136/bmjresp-2016-000158.
13
Agoston-Coldea L, Lupu S, Mocan T. Pulmonary artery stiffness by cardiac magnetic resonance imaging predicts major adverse cardiovascular events in patients with chronic obstructive pulmonary disease[J]. Sci Rep, 2018, 8(1): 14447. DOI: 10.1038/s41598-018-32784-6.
14
Tannus-Silva DG, Rabahi MF. State of the art review of the right ventricle in COPD patients: It is time to look closer[J]. Lung, 2017, 195(1): 9-17. DOI: 10.1007/s00408-016-9961-5.
15
Kanar BG, Ozmen I, Yildirim EO, et al. Right ventricular functional improvement after pulmonary rehabilitation program in patients with COPD determined by speckle tracking echocardiography[J]. Arq Bras Cardiol, 2018, 111(3): 375-381. DOI: 10.5935/abc.20180123.
16
Vonk NA, Marcus JT, Roseboom B, et al. The effect of right ventricular hypertrophy on left ventricular ejection fraction in pulmonary emphysema[J]. Chest, 1997, 112(3): 640-645. DOI: 10.1378/chest.112.3.640.
17
Rothnie KJ, Yan R, Smeeth L, et al. Risk of myocardial infarction (MI) and death following MI in people with chronic obstructive pulmonary disease (COPD): a systematic review and meta-analysis[J]. BMJ Open, 2015, 5(9): e7824. DOI: 10.1136/bmjopen-2015-007824.
18
Rahaghi FN, Vegas-Sanchez-Ferrero G, Minhas JK, et al. Ventricular geometry from non-contrast non-ECG-gated CT scans: An imaging marker of cardiopulmonary disease in smokers[J]. Acad Radiol, 2017, 24(5): 594-602. DOI: 10.1016/j.acra.2016.12.007.
19
Rijnierse MT, van der Lingen A, de Haan S, et al. Value of CMR and PET in predicting ventricular arrhythmias in ischemic cardiomyopathy patients eligible for ICD[J]. JACC Cardiovasc Imaging, 2020, 13(8): 1755-1766. DOI: 10.1016/j.jcmg.2020.01.026.
20
Moccia S, Banali R, Martini C, et al. Development and testing of a deep learning-based strategy for scar segmentation on CMR-LGE images[J]. MAGMA, 2019, 32(2): 187-195. DOI: 10.1007/s10334-018-0718-4.
21
Ferreira VM, Piechnik SK. CMR parametric mapping as a tool for myocardial tissue characterization[J]. Korean Circ J, 2020, 50(8): 658-676. DOI: 10.4070/kcj.2020.0157.
22
Alter P, JÖrres RA, Watz H, et al. Left ventricular volume and wall stress are linked to lung function impairment in COPD[J]. Int J Cardiol, 2018, 261: 172-178. DOI: 10.1016/j.ijcard.2018.02.074.
23
Vonk-Noordegraaf A, Marcus JT, Holverda S, et al. Early changes of cardiac structure and function in COPD patients with mild hypoxemia[J]. Chest, 2005, 127(6): 1898-1903. DOI: 10.1378/chest.127.6.1898.
24
Selvnayagam JB, Jerosch-Herold M, Porto I, et al. Resting myocardial blood flow is impaired in hibernating myocardium: a magnetic resonance study of quantitative perfusion assessment[J]. Circulation, 2005, 112(21): 3289-3296. DOI: 10.1161/CIRCULATIONAHA.105.549170.
25
Murphy CA, Blyth KG, Chaudhuri R, et al. Assessment of the presence of occult myocardial infarction in chronic obstructive pulmonary disease using contrast-enhanced cardiac magnetic resonance imaging[J]. Respiration, 2009, 78(3): 263-269. DOI: 10.1159/000203354.
26
Bessa LG, Junqueira FP, Bandeira ML, et al. Pulmonary arterial hypertension: use of delayed contrast-enhanced cardiovascular magnetic resonance in risk assessment[J]. Arq Bras Cardiol, 2013, 101(4): 336-343. DOI: 10.5935/abc.20130168.
27
Hong Y, Graham MM, Southern D, et al. The association between chronic obstructive pulmonary disease and coronary artery disease in patients undergoing coronary angiography[J]. COPD, 2019, 16(1): 66-71. DOI: 10.1080/15412555.2019.1566894.
28
Vancheri F, Longo G, Vancheri S, et al. Coronary microvascular dysfunction[J]. J Clin Med, 2020, 9(9): 36-39. DOI: 10.3390/jcm9092880.
29
Nakamori S, Onishi K, Ishida M, et al. Myocardial perfusion reserve is impaired in patients with chronic obstructive pulmonary disease: a comparison to current smokers[J]. Eur Heart J Cardiovasc Imaging, 2014, 15(2): 180-188. DOI: 10.1093/ehjci/jet131.
30
Breuckmann F, Nassenstein K, Bucher C, et al. Systematic analysis of functional and structural changes after coronary microembolization: a cardiac magnetic resonance imaging study[J]. JACC Cardiovasc Imaging, 2009, 2(2): 121-130. DOI: 10.1016/j.jcmg.2008.10.011.

PREV Multiparameter MRI radiomics predicts preoperative peritoneal metastasis in patients with epithelial ovarian cancer
NEXT Quantitative MRI analysis of the effect of vitamin D on fat content in bone marrow changes in alloxan-induced diabetic rabbits
  


LINK


Tel & Fax: +8610-67113815    E-mail: editor@cjmri.cn