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• Original Article •
Quantitative MRI analysis of the effect of vitamin D on fat content in bone marrow changes in alloxan-induced diabetic rabbits
YAN Yuchen  ZHA Yunfei 

Cite this article as: Yan YC, Zha YF. Quantitative MRI analysis of the effect of vitamin D on fat content in bone marrow changes in alloxan-induced diabetic rabbits[J]. Chin J Magn Reson Imaging, 2021, 12(8): 55-59. DOI:10.12015/issn.1674-8034.2021.08.011.


[Abstract] Objective Using iterative decomposition of water and fat with echo asymmetry and least-squares estimation quantitation sequence (IDEAL-IQ) to evaluate the effect of vitamin D supplement on the change of bone marrow fat content in vertebral body in alloxan-induced diabetic rabbits. Materials andMethods Thirty New Zealand male rabbits were divided into 20 experienced rabbits (including alloxan-induced diabetic group (n=10), vitamin D intervention group (n=10), and 10 control rabbits, randomly. After the alloxan-induced diabetic model was modeling successfully, the intervention model was given vitamin D (400 μg/week) orally for 4 weeks, and the control group and the simple diabetic group were given 10ml normal saline by gavage every week. After modeling, all rabbits were experienced the exam of magnetic resonance imaging (T1WI, T2WI, IDEAL-IQ) at 0th, 4th, 8th, 12th, 16th week, and all rabbits' vertebral fat content was calculate at GE AW 4.6 standard workstation. After 16 weeks, all rabbits were killed by air embolization, and the lumbar 5—7 vertebral body was taken for the adipocyte count with HE staining. The ratio of lumbar bone marrow to fat in control group, simple diabetes group and intervention group at each time point and the content of lumbar bone marrow adipocytes under HE staining were measured by repeated measurement ANOVA. Pearson correlation analysis was performed on FF value and bone marrow adipocyte content obtained at 16 weeks, and P＜0.05 was considered statistically significant.Results The percentage of bone marrow fat in the vertebral body measured by IDEA-IQ at 0th and 16th week was significantly different between the diabetic group and the vitamin D intervention groups (F=4.971, P＜0.05; F=3.055, P＜0.01), the control group has no statistical differences in vertebral fat fraction at every examination point; However, there was no statistically significant difference in FF value between the diabetic group and the vitamin D intervention group at 16th week (t=2.390, P=0.06). Pearson correlation analysis showed that there was a positive correlation between the fat fraction (FF) of lumbar bone marrow and the number of fat after HE staining at the 16th week in diabetic rabbits (r=0.828, P＜0.05).Conclusions IDEAL-IQ technique is feasible to evaluate the change of bone marrow fat content in alloxan-induced diabetic rabbits with vitamin D supplement. After 16 weeks of vitamin D supplement, the change of bone marrow fat content showed a decreasing trend.
[Keywords] diabetes mellitus；vertebral marrow；vitamin D；fat fraction；magnetic resonance imaging

YAN Yuchen   ZHA Yunfei*  

Renmin Hospital of Wuhan University, Wuhan 430060, China

Zha YF, E-mail: zhayunfei999@126.com

Conflicts of interest   None.

ACKNOWLEDGMENTS This work was part of National Natural Science Foundation of China (No. 81871332).
Received  2021-04-08
Accepted  2021-05-08
DOI: 10.12015/issn.1674-8034.2021.08.011
Cite this article as: Yan YC, Zha YF. Quantitative MRI analysis of the effect of vitamin D on fat content in bone marrow changes in alloxan-induced diabetic rabbits[J]. Chin J Magn Reson Imaging, 2021, 12(8): 55-59. DOI:10.12015/issn.1674-8034.2021.08.011.

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